In vivo biodistribution analysis of transmission competent and defective RNA virus-based episomal vector

RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro . However, little is known about its tissue tropism and persistence of gene expression in vivo . Here, to evaluate the feasibility of REVec for...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Scientific reports Ročník 10; číslo 1; s. 5890 - 12
Hlavní autoři:	Komatsu, Yumiko, Tanaka, Chiaki, Komorizono, Ryo, Tomonaga, Keizo
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 03.04.2020 Nature Publishing Group Nature Portfolio
Témata:	631/1647/2300 631/326/596/2561 Animals Animals, Genetically Modified Antibodies, Neutralizing - blood Antibodies, Viral - blood Astrocytes Astrocytes - metabolism Body weight Brain - metabolism CD8 antigen Feasibility Studies Feces - virology Female Gene Expression Gene transfer Genetic Vectors - administration & dosage Genetic Vectors - genetics Genetic Vectors - isolation & purification Genetic Vectors - pharmacokinetics Humanities and Social Sciences Lymphocytes T Microglia Microglia - metabolism multidisciplinary Neurons - metabolism Pathogenicity Pathogens Plasmids - genetics Rats Rats, Inbred Lew RNA viruses RNA Viruses - genetics RNA Viruses - immunology RNA Viruses - isolation & purification Science Science (multidisciplinary) Tissue Distribution Transduction, Genetic Transgenes - genetics Tropism Virus Replication Virus Shedding
ISSN:	2045-2322, 2045-2322
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro . However, little is known about its tissue tropism and persistence of gene expression in vivo . Here, to evaluate the feasibility of REVec for in vivo gene delivery, we conducted biodistribution analysis of transmission competent REVec and transmission defective ΔG-REVec in Lewis rats. Following intracranial administration of REVec, transgene expression was detected in various tissues. In contrast, transgene expression was only observed in the brain after ΔG-REVec administration. Low levels of vector shedding in the feces and blood and of neutralizing antibody in the serum were detected after REVec injection. In the brain, microglia, astrocytes and neurons were susceptible to REVec-mediated transduction. However, the animals administered with REVec, but not with ΔG-REVec showed a significant decrease in body weight compared to mock treated animals. Additionally, CD8 T cell infiltration was observed in the brain of these animals. In summary, we demonstrated that REVec promotes long-term transgene expression in vivo without causing high vector shedding or neutralizing antibody production; however, suggests the need to attenuate vector associated pathogenicity in the future.
AbstractList	RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro . However, little is known about its tissue tropism and persistence of gene expression in vivo . Here, to evaluate the feasibility of REVec for in vivo gene delivery, we conducted biodistribution analysis of transmission competent REVec and transmission defective ΔG-REVec in Lewis rats. Following intracranial administration of REVec, transgene expression was detected in various tissues. In contrast, transgene expression was only observed in the brain after ΔG-REVec administration. Low levels of vector shedding in the feces and blood and of neutralizing antibody in the serum were detected after REVec injection. In the brain, microglia, astrocytes and neurons were susceptible to REVec-mediated transduction. However, the animals administered with REVec, but not with ΔG-REVec showed a significant decrease in body weight compared to mock treated animals. Additionally, CD8 T cell infiltration was observed in the brain of these animals. In summary, we demonstrated that REVec promotes long-term transgene expression in vivo without causing high vector shedding or neutralizing antibody production; however, suggests the need to attenuate vector associated pathogenicity in the future. RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro. However, little is known about its tissue tropism and persistence of gene expression in vivo. Here, to evaluate the feasibility of REVec for in vivo gene delivery, we conducted biodistribution analysis of transmission competent REVec and transmission defective ΔG-REVec in Lewis rats. Following intracranial administration of REVec, transgene expression was detected in various tissues. In contrast, transgene expression was only observed in the brain after ΔG-REVec administration. Low levels of vector shedding in the feces and blood and of neutralizing antibody in the serum were detected after REVec injection. In the brain, microglia, astrocytes and neurons were susceptible to REVec-mediated transduction. However, the animals administered with REVec, but not with ΔG-REVec showed a significant decrease in body weight compared to mock treated animals. Additionally, CD8 T cell infiltration was observed in the brain of these animals. In summary, we demonstrated that REVec promotes long-term transgene expression in vivo without causing high vector shedding or neutralizing antibody production; however, suggests the need to attenuate vector associated pathogenicity in the future. Abstract RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro. However, little is known about its tissue tropism and persistence of gene expression in vivo. Here, to evaluate the feasibility of REVec for in vivo gene delivery, we conducted biodistribution analysis of transmission competent REVec and transmission defective ΔG-REVec in Lewis rats. Following intracranial administration of REVec, transgene expression was detected in various tissues. In contrast, transgene expression was only observed in the brain after ΔG-REVec administration. Low levels of vector shedding in the feces and blood and of neutralizing antibody in the serum were detected after REVec injection. In the brain, microglia, astrocytes and neurons were susceptible to REVec-mediated transduction. However, the animals administered with REVec, but not with ΔG-REVec showed a significant decrease in body weight compared to mock treated animals. Additionally, CD8 T cell infiltration was observed in the brain of these animals. In summary, we demonstrated that REVec promotes long-term transgene expression in vivo without causing high vector shedding or neutralizing antibody production; however, suggests the need to attenuate vector associated pathogenicity in the future. RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro. However, little is known about its tissue tropism and persistence of gene expression in vivo. Here, to evaluate the feasibility of REVec for in vivo gene delivery, we conducted biodistribution analysis of transmission competent REVec and transmission defective ΔG-REVec in Lewis rats. Following intracranial administration of REVec, transgene expression was detected in various tissues. In contrast, transgene expression was only observed in the brain after ΔG-REVec administration. Low levels of vector shedding in the feces and blood and of neutralizing antibody in the serum were detected after REVec injection. In the brain, microglia, astrocytes and neurons were susceptible to REVec-mediated transduction. However, the animals administered with REVec, but not with ΔG-REVec showed a significant decrease in body weight compared to mock treated animals. Additionally, CD8 T cell infiltration was observed in the brain of these animals. In summary, we demonstrated that REVec promotes long-term transgene expression in vivo without causing high vector shedding or neutralizing antibody production; however, suggests the need to attenuate vector associated pathogenicity in the future.RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro. However, little is known about its tissue tropism and persistence of gene expression in vivo. Here, to evaluate the feasibility of REVec for in vivo gene delivery, we conducted biodistribution analysis of transmission competent REVec and transmission defective ΔG-REVec in Lewis rats. Following intracranial administration of REVec, transgene expression was detected in various tissues. In contrast, transgene expression was only observed in the brain after ΔG-REVec administration. Low levels of vector shedding in the feces and blood and of neutralizing antibody in the serum were detected after REVec injection. In the brain, microglia, astrocytes and neurons were susceptible to REVec-mediated transduction. However, the animals administered with REVec, but not with ΔG-REVec showed a significant decrease in body weight compared to mock treated animals. Additionally, CD8 T cell infiltration was observed in the brain of these animals. In summary, we demonstrated that REVec promotes long-term transgene expression in vivo without causing high vector shedding or neutralizing antibody production; however, suggests the need to attenuate vector associated pathogenicity in the future.
ArticleNumber	5890
Author	Komatsu, Yumiko Tomonaga, Keizo Komorizono, Ryo Tanaka, Chiaki
Author_xml	– sequence: 1 givenname: Yumiko surname: Komatsu fullname: Komatsu, Yumiko email: ykomatsu@infront.kyoto-u.ac.jp organization: Laboratory of RNA Viruses, Department of Virus Research, Institute for Frontier Life and Medical Sciences (inFront), Kyoto University, Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research, Kyoto University – sequence: 2 givenname: Chiaki surname: Tanaka fullname: Tanaka, Chiaki organization: Laboratory of RNA Viruses, Department of Virus Research, Institute for Frontier Life and Medical Sciences (inFront), Kyoto University – sequence: 3 givenname: Ryo surname: Komorizono fullname: Komorizono, Ryo organization: Laboratory of RNA Viruses, Department of Virus Research, Institute for Frontier Life and Medical Sciences (inFront), Kyoto University, Department of Mammalian Regulatory Network, Graduate School of Biostudies, Kyoto University – sequence: 4 givenname: Keizo surname: Tomonaga fullname: Tomonaga, Keizo email: tomonaga@infront.kyoto-u.ac.jp organization: Laboratory of RNA Viruses, Department of Virus Research, Institute for Frontier Life and Medical Sciences (inFront), Kyoto University, Department of Mammalian Regulatory Network, Graduate School of Biostudies, Kyoto University, Department of Molecular Virology, Graduate School of Medicine, Kyoto University
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/32246020$$D View this record in MEDLINE/PubMed
BookMark	eNp9kktr3DAUhUVJadI0f6CLYuimG7d6WA9vCiH0MRBaKO1ayNLVRINtTSXbkH8fTZy0SRbRRuLqO4cj3fsaHY1xBITeEvyRYKY-5YbwVtWY4lpQwXAtX6ATihteU0bp0YPzMTrLeYfL4rRtSPsKHZdqI4r0BF1txmoJS6y6EF3IUwrdPIU4VmY0_XUOuYq-mpIZ8xByPlzYOOxhgnEqiKsceLBTWKD69eO8OKU5153J4CrYhxwH01dLAWJ6g15602c4u9tP0Z-vX35ffK8vf37bXJxf1lY0fKpBGHDUONIqybyznfOKSa4sdMzJlnrpaWcp4aJlUnnLqXSmc4r5FlyDMTtFm9XXRbPT-xQGk651NEHfFmLaapOmYHvQApRUQIEwzxomieKWmEYQZwk3yoni9Xn12s_dAM6WRyfTPzJ9fDOGK72Ni5aEcizbYvDhziDFvzPkSZdftND3ZoQ4Z02ZElS1uJEFff8E3cU5lSasFGtaKkih3j1M9C_KfUMLoFbApphzAq9tmMyhoyVg6DXB-jA-eh0fXST6dnz0IQF9Ir13f1bEVlEu8LiF9D_2M6obAhDY8Q
CitedBy_id	crossref_primary_10_3390_v14102236 crossref_primary_10_1016_j_omtm_2023_01_008 crossref_primary_10_1080_17460441_2023_2195164 crossref_primary_10_1128_JVI_00936_21 crossref_primary_10_1007_s00253_024_13327_8 crossref_primary_10_1128_JVI_02221_20 crossref_primary_10_1002_1873_3468_14290 crossref_primary_10_1111_1348_0421_12946
Cites_doi	10.1038/nm1549 10.1038/mt.2008.76 10.1146/annurev-med-012017-043332 10.1016/j.chom.2012.04.009 10.1128/JVI.00334-07 10.1038/gt.2009.157 10.1111/1348-0421.12505 10.1128/JVI.72.11.8884-8892.1998 10.1126/scitranslmed.3002865 10.1038/srep26154 10.1007/978-1-4939-3271-9_17 10.1159/000024926 10.1093/infdis/148.2.305 10.1056/NEJMoa1708483 10.1038/gt.2015.108 10.1016/S0140-6736(16)30371-3 10.1016/S0140-6736(17)31868-8 10.1128/JVI.66.2.992-998.1992 10.1097/00005072-198911000-00005 10.1016/j.omtm.2019.05.010 10.1099/0022-1317-66-3-503 10.1128/JVI.69.2.741-747.1995 10.1056/NEJMoa1706198 10.1096/fj.03-0764fje 10.1128/JVI.61.11.3431-3440.1987 10.1182/blood-2008-07-167510 10.1111/j.1439-0450.1997.tb00962.x 10.1111/1348-0421.12602 10.1111/j.1365-2990.2008.00940.x 10.1128/JVI.65.11.6154-6164.1991 10.1056/NEJMoa1708538 10.1007/BF02122108 10.1006/mthe.2001.0460 10.1128/JVI.75.2.943-951.2001 10.1128/JVI.05554-11 10.4049/jimmunol.153.2.818
ContentType	Journal Article
Copyright	The Author(s) 2020 The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml	– notice: The Author(s) 2020 – notice: The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID	C6C AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7X7 7XB 88A 88E 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 5PM DOA
DOI	10.1038/s41598-020-62630-7
DatabaseName	Springer Nature OA Free Journals CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials - QC ProQuest SciTech Premium Collection Natural Science Collection Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea ProQuest Health & Medical Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Biological Science Collection ProQuest Health & Medical Collection Medical Database Science Database Biological Science Database ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic Publicly Available Content Database MEDLINE CrossRef
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
EISSN	2045-2322
EndPage	12
ExternalDocumentID	oai_doaj_org_article_6e878e2e13f3437185c1a461dc15a8d6 PMC7125079 32246020 10_1038_s41598_020_62630_7
Genre	Research Support, Non-U.S. Gov't Journal Article
GroupedDBID	0R~ 3V. 4.4 53G 5VS 7X7 88A 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAJSJ AAKDD ABDBF ABUWG ACGFS ACSMW ACUHS ADBBV ADRAZ AENEX AEUYN AFKRA AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI C6C CCPQU DIK DWQXO EBD EBLON EBS ESX FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE KQ8 LK8 M0L M1P M2P M48 M7P M~E NAO OK1 PIMPY PQQKQ PROAC PSQYO RNT RNTTT RPM SNYQT UKHRP AASML AAYXX AFFHD AFPKN CITATION PHGZM PHGZT PJZUB PPXIY PQGLB CGR CUY CVF ECM EIF NPM 7XB 8FK K9. PKEHL PQEST PQUKI PRINS Q9U 7X8 PUEGO 5PM
ID	FETCH-LOGICAL-c645t-e6aed2ad19873fdcbdf83758ceb3d792f7f2bc21569378fc527dabd83f9ed4003
IEDL.DBID	M7P
ISICitedReferencesCount	8
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000563490900011&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	2045-2322
IngestDate	Tue Oct 14 18:59:25 EDT 2025 Tue Nov 04 01:35:37 EST 2025 Fri Sep 05 08:34:09 EDT 2025 Tue Oct 07 07:38:39 EDT 2025 Thu Apr 03 07:10:00 EDT 2025 Sat Nov 29 05:32:29 EST 2025 Tue Nov 18 22:14:22 EST 2025 Fri Feb 21 02:37:02 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	1
Language	English
License	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c645t-e6aed2ad19873fdcbdf83758ceb3d792f7f2bc21569378fc527dabd83f9ed4003
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
OpenAccessLink	https://www.proquest.com/docview/2386349261?pq-origsite=%requestingapplication%
PMID	32246020
PQID	2386349261
PQPubID	2041939
PageCount	12
ParticipantIDs	doaj_primary_oai_doaj_org_article_6e878e2e13f3437185c1a461dc15a8d6 pubmedcentral_primary_oai_pubmedcentral_nih_gov_7125079 proquest_miscellaneous_2386289047 proquest_journals_2386349261 pubmed_primary_32246020 crossref_citationtrail_10_1038_s41598_020_62630_7 crossref_primary_10_1038_s41598_020_62630_7 springer_journals_10_1038_s41598_020_62630_7
PublicationCentury	2000
PublicationDate	2020-04-03
PublicationDateYYYYMMDD	2020-04-03
PublicationDate_xml	– month: 04 year: 2020 text: 2020-04-03 day: 03
PublicationDecade	2020
PublicationPlace	London
PublicationPlace_xml	– name: London – name: England
PublicationTitle	Scientific reports
PublicationTitleAbbrev	Sci Rep
PublicationTitleAlternate	Sci Rep
PublicationYear	2020
Publisher	Nature Publishing Group UK Nature Publishing Group Nature Portfolio
Publisher_xml	– name: Nature Publishing Group UK – name: Nature Publishing Group – name: Nature Portfolio
References	Ackermann, Staeheli, Schneider (CR21) 2007; 81 Anguela, High (CR1) 2019; 70 George (CR6) 2017; 377 Herzog, Wonigeit, Frese, Hedrich, Rott (CR27) 1985; 66 Stoyloff, Bode, Borchers, Ludwig (CR31) 1998; 41 Kim, Grunke, Jankowsky (CR35) 2016; 1382 Durrwald, Ludwig (CR8) 1997; 44 Rangarajan (CR7) 2017; 377 Bilzer, Stitz (CR19) 1994; 153 Mingozzi (CR33) 2009; 114 Narayan, Herzog, Frese, Scheefers, Rott (CR26) 1983; 148 Zincarelli, Soltys, Rengo, Rabinowitz (CR25) 2008; 16 Carbone, Park, Rubin, Waltrip, Vogelsang (CR18) 1991; 65 Herzog, Kompter, Frese, Rott (CR20) 1984; 173 Honda (CR16) 2016; 6 Mingozzi (CR32) 2007; 13 Mendell (CR5) 2017; 377 Carbone, Duchala, Narayan (CR22) 1989; 48 Wu, Sundaresan, Iyer, Gambhir (CR36) 2001; 4 Carbone, Duchala, Griffin, Kincaid, Narayan (CR23) 1987; 61 Fujino (CR12) 2017; 61 Russell (CR2) 2017; 390 Werner-Keišs (CR24) 2008; 34 Bennett (CR4) 2012; 4 Ayuso (CR34) 2010; 17 Bennett (CR3) 2016; 388 Sakai (CR14) 2018; 62 Hans (CR10) 2004; 18 Ikeda (CR13) 2016; 23 Stitz (CR30) 1998; 72 Matsumoto (CR9) 2012; 11 Furrer, Bilzer, Stitz, Planz (CR28) 2001; 75 Komatsu (CR15) 2019; 14 Hatalski, Kliche, Stitz, Lipkin (CR29) 1995; 69 Shankar (CR17) 1992; 66 Daito (CR11) 2011; 85 M Sakai (62630_CR14) 2018; 62 T Daito (62630_CR11) 2011; 85 JY Kim (62630_CR35) 2016; 1382 J Bennett (62630_CR3) 2016; 388 T Bilzer (62630_CR19) 1994; 153 K Fujino (62630_CR12) 2017; 61 R Durrwald (62630_CR8) 1997; 44 T Honda (62630_CR16) 2016; 6 KM Carbone (62630_CR18) 1991; 65 JC Wu (62630_CR36) 2001; 4 Y Komatsu (62630_CR15) 2019; 14 S Herzog (62630_CR20) 1984; 173 LA George (62630_CR6) 2017; 377 A Ackermann (62630_CR21) 2007; 81 A Hans (62630_CR10) 2004; 18 S Rangarajan (62630_CR7) 2017; 377 F Mingozzi (62630_CR33) 2009; 114 J Bennett (62630_CR4) 2012; 4 V Shankar (62630_CR17) 1992; 66 E Ayuso (62630_CR34) 2010; 17 F Mingozzi (62630_CR32) 2007; 13 CG Hatalski (62630_CR29) 1995; 69 Y Matsumoto (62630_CR9) 2012; 11 L Stitz (62630_CR30) 1998; 72 Y Ikeda (62630_CR13) 2016; 23 XM Anguela (62630_CR1) 2019; 70 S Russell (62630_CR2) 2017; 390 R Stoyloff (62630_CR31) 1998; 41 O Narayan (62630_CR26) 1983; 148 JR Mendell (62630_CR5) 2017; 377 KM Carbone (62630_CR22) 1989; 48 E Furrer (62630_CR28) 2001; 75 N Werner-Keišs (62630_CR24) 2008; 34 KM Carbone (62630_CR23) 1987; 61 C Zincarelli (62630_CR25) 2008; 16 S Herzog (62630_CR27) 1985; 66
References_xml	– volume: 13 start-page: 419 year: 2007 end-page: 422 ident: CR32 article-title: CD18+ T-cell responses to adeno-associated virus capsid in humans publication-title: Nat. Med. doi: 10.1038/nm1549 – volume: 16 start-page: 1073 year: 2008 end-page: 1080 ident: CR25 article-title: Analysis of AAV serotypes 1-9 mediated gene expression and tropism in mice after systemic injection publication-title: Mol. Ther. doi: 10.1038/mt.2008.76 – volume: 70 start-page: 273 year: 2019 end-page: 288 ident: CR1 article-title: Entering the Modern Era of Gene Therapy publication-title: Annu. Rev. Med. doi: 10.1146/annurev-med-012017-043332 – volume: 11 start-page: 492 year: 2012 end-page: 503 ident: CR9 article-title: Bornavirus closely associates and segregates with host chromosomes to ensure persistent intranuclear infection publication-title: Cell Host Microbe doi: 10.1016/j.chom.2012.04.009 – volume: 81 start-page: 7933 year: 2007 end-page: 7940 ident: CR21 article-title: Adaptation of Borna Disease Virus to New Host Species Attributed to Altered Regulation of Viral Polymerase Activity publication-title: J. Virol. doi: 10.1128/JVI.00334-07 – volume: 17 start-page: 503 year: 2010 end-page: 510 ident: CR34 article-title: High AAV vector purity results in serotype- and tissue-independent enhancement of transduction efficiency publication-title: Gene Ther. doi: 10.1038/gt.2009.157 – volume: 61 start-page: 380 year: 2017 end-page: 386 ident: CR12 article-title: Generation of a non-transmissive Borna disease virus vector lacking both matrix and glycoprotein genes publication-title: Microbiol. Immunol. doi: 10.1111/1348-0421.12505 – volume: 72 start-page: 8884 year: 1998 end-page: 8892 ident: CR30 article-title: A functional role for neutralizing antibodies in Borna disease: influence on virus tropism outside the central nervous system publication-title: J. Virol. doi: 10.1128/JVI.72.11.8884-8892.1998 – volume: 4 start-page: 120ra15 year: 2012 ident: CR4 article-title: AAV2 Gene Therapy Readministration in Three Adults with Congenital Blindness publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.3002865 – volume: 6 year: 2016 ident: CR16 article-title: Long-term expression of miRNA for RNA interference using a novel vector system based on a negative-strand RNA virus publication-title: Sci. Rep. doi: 10.1038/srep26154 – volume: 1382 start-page: 239 year: 2016 end-page: 250 ident: CR35 article-title: Widespread neuronal transduction of the rodent CNS via neonatal viral injection publication-title: Methods Mol. Biol. doi: 10.1007/978-1-4939-3271-9_17 – volume: 41 start-page: 135 year: 1998 end-page: 140 ident: CR31 article-title: Neutralization of borna disease virus depends upon terminal carbohydrate residues (α-D-Man, β-D-GlcNAc) of glycoproteins gp17 and gp94 publication-title: Intervirology doi: 10.1159/000024926 – volume: 148 start-page: 305 year: 1983 end-page: 315 ident: CR26 article-title: Pathogenesis of borna disease in rats: Immune-mediated viral ophthalmoencephalopathy causing blindness and behavioral abnormalities publication-title: J. Infect. Dis. doi: 10.1093/infdis/148.2.305 – volume: 377 start-page: 2519 year: 2017 end-page: 2530 ident: CR7 article-title: AAV5-factor VIII gene transfer in severe hemophilia a publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1708483 – volume: 23 start-page: 256 year: 2016 end-page: 262 ident: CR13 article-title: A novel intranuclear RNA vector system for long-term stem cell modification publication-title: Gene Ther. doi: 10.1038/gt.2015.108 – volume: 388 start-page: 661 year: 2016 end-page: 672 ident: CR3 article-title: Safety and durability of effect of contralateral-eye administration of AAV2 gene therapy in patients with childhood-onset blindness caused by RPE65 mutations: a follow-on phase 1 trial publication-title: Lancet doi: 10.1016/S0140-6736(16)30371-3 – volume: 390 start-page: 849 year: 2017 end-page: 860 ident: CR2 article-title: Efficacy and safety of voretigene neparvovec (AAV2-hRPE65v2) in patients with RPE65-mediated inherited retinal dystrophy: a randomised, controlled, open-label, phase 3 trial publication-title: Lancet doi: 10.1016/S0140-6736(17)31868-8 – volume: 66 start-page: 992 year: 1992 end-page: 998 ident: CR17 article-title: Kinetics of virus spread and changes in levels of several cytokine mRNAs in the brain after intranasal infection of rats with Borna disease virus publication-title: J. Virol. doi: 10.1128/JVI.66.2.992-998.1992 – volume: 48 start-page: 631 year: 1989 end-page: 644 ident: CR22 article-title: Astrocytes and schwann cells are virus-host cells in the nervous system of rats with borna disease publication-title: J. Neuropathol. Exp. Neurol. doi: 10.1097/00005072-198911000-00005 – volume: 14 start-page: 47 year: 2019 end-page: 55 ident: CR15 article-title: RNA Virus-Based Episomal Vector with a Fail-Safe Switch Facilitating Efficient Genetic Modification and Differentiation of iPSCs publication-title: Mol. Ther. - Methods Clin. Dev. doi: 10.1016/j.omtm.2019.05.010 – volume: 66 start-page: 503 year: 1985 end-page: 508 ident: CR27 article-title: Effect of Borna disease virus infection on athymic rats publication-title: J. Gen. Virol. doi: 10.1099/0022-1317-66-3-503 – volume: 69 start-page: 741 year: 1995 end-page: 747 ident: CR29 article-title: Neutralizing antibodies in Borna disease virus-infected rats publication-title: J. Virol. doi: 10.1128/JVI.69.2.741-747.1995 – volume: 377 start-page: 1713 year: 2017 end-page: 1722 ident: CR5 article-title: Single-dose gene-replacement therapy for spinal muscular atrophy publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1706198 – volume: 18 start-page: 863 year: 2004 end-page: 865 ident: CR10 article-title: Persistent, noncytolytic infection of neurons by Borna disease virus interferes with ERK 1/2 signaling and abrogates BDNF-induced synaptogenesis publication-title: FASEB J. doi: 10.1096/fj.03-0764fje – volume: 61 start-page: 3431 year: 1987 end-page: 3440 ident: CR23 article-title: Pathogenesis of Borna disease in rats: evidence that intra-axonal spread is the major route for virus dissemination and the determinant for disease incubation publication-title: J. Virol. doi: 10.1128/JVI.61.11.3431-3440.1987 – volume: 114 start-page: 2077 year: 2009 end-page: 2086 ident: CR33 article-title: AAV-1-mediated gene transfer to skeletal muscle in humans results in dose-dependent activation of capsid-specific T cells publication-title: Blood doi: 10.1182/blood-2008-07-167510 – volume: 44 start-page: 147 year: 1997 end-page: 184 ident: CR8 article-title: Borna Disease Virus (BDV), a (Zoonotic?) Worldwide Pathogen. A review of the History of the Disease and the Virus Infection with Comprehensive Bibliography publication-title: J. Vet. Med. Ser. B doi: 10.1111/j.1439-0450.1997.tb00962.x – volume: 62 start-page: 467 year: 2018 end-page: 472 ident: CR14 article-title: Degradation of amyloid β peptide by neprilysin expressed from Borna disease virus vector publication-title: Microbiol. Immunol. doi: 10.1111/1348-0421.12602 – volume: 34 start-page: 590 year: 2008 end-page: 602 ident: CR24 article-title: Restricted expression of Borna disease virus glycoprotein in brains of experimentally infected Lewis rats publication-title: Neuropathol. Appl. Neurobiol. doi: 10.1111/j.1365-2990.2008.00940.x – volume: 65 start-page: 6154 year: 1991 end-page: 6164 ident: CR18 article-title: Borna disease: Association with a maturation defect in the cellular immune response publication-title: J. Virol. doi: 10.1128/JVI.65.11.6154-6164.1991 – volume: 153 start-page: 818 year: 1994 end-page: 823 ident: CR19 article-title: Immune-mediated brain atrophy: CD8+ T cells contribute to tissue destruction during Borna disease publication-title: J. Immunol. – volume: 377 start-page: 2215 year: 2017 end-page: 2227 ident: CR6 article-title: Hemophilia B gene therapy with a high-specific-activity factor IX variant publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1708538 – volume: 173 start-page: 171 year: 1984 end-page: 177 ident: CR20 article-title: Replication of Borna disease virus in rats: age-dependent differences in tissue distribution publication-title: Med. Microbiol. Immunol. doi: 10.1007/BF02122108 – volume: 4 start-page: 297 year: 2001 end-page: 306 ident: CR36 article-title: Noninvasive optical imaging of firefly luciferase reporter gene expression in skeletal muscles of living mice publication-title: Mol. Ther. doi: 10.1006/mthe.2001.0460 – volume: 75 start-page: 943 year: 2001 end-page: 951 ident: CR28 article-title: Neutralizing Antibodies in Persistent Borna Disease Virus Infection: Prophylactic Effect of gp94-Specific Monoclonal Antibodies in Preventing Encephalitis publication-title: J. Virol. doi: 10.1128/JVI.75.2.943-951.2001 – volume: 85 start-page: 12170 year: 2011 end-page: 12178 ident: CR11 article-title: A Novel Borna Disease Virus Vector System That Stably Expresses Foreign Proteins from an Intercistronic Noncoding Region publication-title: J. Virol. doi: 10.1128/JVI.05554-11 – volume: 1382 start-page: 239 year: 2016 ident: 62630_CR35 publication-title: Methods Mol. Biol. doi: 10.1007/978-1-4939-3271-9_17 – volume: 66 start-page: 992 year: 1992 ident: 62630_CR17 publication-title: J. Virol. doi: 10.1128/JVI.66.2.992-998.1992 – volume: 23 start-page: 256 year: 2016 ident: 62630_CR13 publication-title: Gene Ther. doi: 10.1038/gt.2015.108 – volume: 390 start-page: 849 year: 2017 ident: 62630_CR2 publication-title: Lancet doi: 10.1016/S0140-6736(17)31868-8 – volume: 17 start-page: 503 year: 2010 ident: 62630_CR34 publication-title: Gene Ther. doi: 10.1038/gt.2009.157 – volume: 62 start-page: 467 year: 2018 ident: 62630_CR14 publication-title: Microbiol. Immunol. doi: 10.1111/1348-0421.12602 – volume: 75 start-page: 943 year: 2001 ident: 62630_CR28 publication-title: J. Virol. doi: 10.1128/JVI.75.2.943-951.2001 – volume: 65 start-page: 6154 year: 1991 ident: 62630_CR18 publication-title: J. Virol. doi: 10.1128/JVI.65.11.6154-6164.1991 – volume: 34 start-page: 590 year: 2008 ident: 62630_CR24 publication-title: Neuropathol. Appl. Neurobiol. doi: 10.1111/j.1365-2990.2008.00940.x – volume: 69 start-page: 741 year: 1995 ident: 62630_CR29 publication-title: J. Virol. doi: 10.1128/JVI.69.2.741-747.1995 – volume: 148 start-page: 305 year: 1983 ident: 62630_CR26 publication-title: J. Infect. Dis. doi: 10.1093/infdis/148.2.305 – volume: 85 start-page: 12170 year: 2011 ident: 62630_CR11 publication-title: J. Virol. doi: 10.1128/JVI.05554-11 – volume: 377 start-page: 1713 year: 2017 ident: 62630_CR5 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1706198 – volume: 4 start-page: 120ra15 year: 2012 ident: 62630_CR4 publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.3002865 – volume: 61 start-page: 380 year: 2017 ident: 62630_CR12 publication-title: Microbiol. Immunol. doi: 10.1111/1348-0421.12505 – volume: 388 start-page: 661 year: 2016 ident: 62630_CR3 publication-title: Lancet doi: 10.1016/S0140-6736(16)30371-3 – volume: 44 start-page: 147 year: 1997 ident: 62630_CR8 publication-title: J. Vet. Med. Ser. B doi: 10.1111/j.1439-0450.1997.tb00962.x – volume: 14 start-page: 47 year: 2019 ident: 62630_CR15 publication-title: Mol. Ther. - Methods Clin. Dev. doi: 10.1016/j.omtm.2019.05.010 – volume: 153 start-page: 818 year: 1994 ident: 62630_CR19 publication-title: J. Immunol. doi: 10.4049/jimmunol.153.2.818 – volume: 377 start-page: 2519 year: 2017 ident: 62630_CR7 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1708483 – volume: 81 start-page: 7933 year: 2007 ident: 62630_CR21 publication-title: J. Virol. doi: 10.1128/JVI.00334-07 – volume: 173 start-page: 171 year: 1984 ident: 62630_CR20 publication-title: Med. Microbiol. Immunol. doi: 10.1007/BF02122108 – volume: 16 start-page: 1073 year: 2008 ident: 62630_CR25 publication-title: Mol. Ther. doi: 10.1038/mt.2008.76 – volume: 377 start-page: 2215 year: 2017 ident: 62630_CR6 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1708538 – volume: 13 start-page: 419 year: 2007 ident: 62630_CR32 publication-title: Nat. Med. doi: 10.1038/nm1549 – volume: 4 start-page: 297 year: 2001 ident: 62630_CR36 publication-title: Mol. Ther. doi: 10.1006/mthe.2001.0460 – volume: 70 start-page: 273 year: 2019 ident: 62630_CR1 publication-title: Annu. Rev. Med. doi: 10.1146/annurev-med-012017-043332 – volume: 18 start-page: 863 year: 2004 ident: 62630_CR10 publication-title: FASEB J. doi: 10.1096/fj.03-0764fje – volume: 6 year: 2016 ident: 62630_CR16 publication-title: Sci. Rep. doi: 10.1038/srep26154 – volume: 61 start-page: 3431 year: 1987 ident: 62630_CR23 publication-title: J. Virol. doi: 10.1128/JVI.61.11.3431-3440.1987 – volume: 66 start-page: 503 year: 1985 ident: 62630_CR27 publication-title: J. Gen. Virol. doi: 10.1099/0022-1317-66-3-503 – volume: 41 start-page: 135 year: 1998 ident: 62630_CR31 publication-title: Intervirology doi: 10.1159/000024926 – volume: 48 start-page: 631 year: 1989 ident: 62630_CR22 publication-title: J. Neuropathol. Exp. Neurol. doi: 10.1097/00005072-198911000-00005 – volume: 114 start-page: 2077 year: 2009 ident: 62630_CR33 publication-title: Blood doi: 10.1182/blood-2008-07-167510 – volume: 11 start-page: 492 year: 2012 ident: 62630_CR9 publication-title: Cell Host Microbe doi: 10.1016/j.chom.2012.04.009 – volume: 72 start-page: 8884 year: 1998 ident: 62630_CR30 publication-title: J. Virol. doi: 10.1128/JVI.72.11.8884-8892.1998
SSID	ssj0000529419
Score	2.3349712
Snippet	RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro .... RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro.... Abstract RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in...
SourceID	doaj pubmedcentral proquest pubmed crossref springer
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher
StartPage	5890
SubjectTerms	631/1647/2300 631/326/596/2561 Animals Animals, Genetically Modified Antibodies, Neutralizing - blood Antibodies, Viral - blood Astrocytes Astrocytes - metabolism Body weight Brain - metabolism CD8 antigen Feasibility Studies Feces - virology Female Gene Expression Gene transfer Genetic Vectors - administration & dosage Genetic Vectors - genetics Genetic Vectors - isolation & purification Genetic Vectors - pharmacokinetics Humanities and Social Sciences Lymphocytes T Microglia Microglia - metabolism multidisciplinary Neurons - metabolism Pathogenicity Pathogens Plasmids - genetics Rats Rats, Inbred Lew RNA viruses RNA Viruses - genetics RNA Viruses - immunology RNA Viruses - isolation & purification Science Science (multidisciplinary) Tissue Distribution Transduction, Genetic Transgenes - genetics Tropism Virus Replication Virus Shedding
SummonAdditionalLinks	– databaseName: Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1bi9UwEA6yKPgi3q2uEsE3LdsmbZI-ruKiLwcRhX0LaSZhC9oupz0H_PdOLue4x-uLr800nc4lM7l9Q8gL1xjumJMlM1aVGI-rsmvAoS1D5TGi98xWsdiEXK3U-Xn34Uqpr3AmLMEDJ8GdCKekwt5q7nnDcSRtbW0aUYOtW6Mggm1j1nNlMpVQvVnX1F2-JVNxdTJjpAq3ycJsiQlelfIgEkXA_t9lmb8elvxpxzQGorPb5FbOIOlp4vwOuebGu-RGqin57R65eD_S7bCdaD9MEFBxc0ErajL8CJ08XUKEQg2HpTJqc-q8IAlQcD6NgfTj6hR7Wm_mMoQ6oO5ymKev-OltXOm_Tz6fvf305l2ZyymUVjTtUjphHDADYZmBe7A9eJydtsrifBpkx7z0rLeYAghMWZS3LZNgelDcdw7Q1fkDcjROo3tEqDPo1h0H7gU0TlqlIECzi9YKWzmoC1LvRKttxhoPJS--6LjnzZVO6tCoDh3VoWVBXu7fuUxIG3-lfh00tqcMKNnxAdqOzraj_2U7BTne6Vtn15015jAiQDYK_Ivn-2ZUSdhJMaObNokm7NA2yMfDZB57TniA6ENOCyIPDOeA1cOWcbiIwN4Ss81KdgV5tTOxH2z9WRSP_4conpCbLPhGOJPEj8nRst64p-S63S7DvH4Wnes7QcQnnw priority: 102 providerName: Directory of Open Access Journals
Title	In vivo biodistribution analysis of transmission competent and defective RNA virus-based episomal vector
URI	https://link.springer.com/article/10.1038/s41598-020-62630-7 https://www.ncbi.nlm.nih.gov/pubmed/32246020 https://www.proquest.com/docview/2386349261 https://www.proquest.com/docview/2386289047 https://pubmed.ncbi.nlm.nih.gov/PMC7125079 https://doaj.org/article/6e878e2e13f3437185c1a461dc15a8d6
Volume	10
WOSCitedRecordID	wos000563490900011&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: DOA dateStart: 20110101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M~E dateStart: 20110101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M7P dateStart: 20110101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: 7X7 dateStart: 20110101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: BENPR dateStart: 20110101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: PIMPY dateStart: 20110101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Science Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M2P dateStart: 20110101 isFulltext: true titleUrlDefault: https://search.proquest.com/sciencejournals providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9NAEB7RFiQuvB-GEi0SN7Bqe-3d9Qm1qBU9NLIqkMLJcvZBI4Ed4iQS_56Z9SZVePTCxYfsJhn7m52ZnVl_A_DG5g23mZVx1mgVoz9O4jI3FnXZJA49-jTTiW82IcdjNZmUVUi49eFY5cYmekNtOk058iN0LYKY9ET6fv4jpq5RVF0NLTT24IBYErg_uldtcyxUxcrTMrwrk3B11KO_onfKaM-UCZ7Ecscfedr-v8Wafx6Z_K1u6t3R2f3_vZEHcC8Eoux40JyHcMu2j-DO0Jry52O4Om_Zerbu2HTWGSLXDX2xWBNYTFjn2JIcHSoKZdyYDhH4EqcYZqwbTCm7HB_jLy1WfUwe0zA7n_Xdd_zrtS8YPIHPZ6efPnyMQ1eGWIu8WMZWNNZkjaFsBXdGT43DTW6hNG7LjSwzJ1021YiGwMhHOV1k0jRTo7grrUGLwZ_Cftu19jkw26B1KLnhTpjcSq2UIYZ3UWihE2vSCNINNrUOlOXUOeNb7UvnXNUDnjXiWXs8axnB2-135gNhx42zTwjy7Uwi2_YfdIuvdVi7tbBKKlTolDuec3TmhU6bXKRGp0WjjIjgcIN0HSxAX1_DHMHr7TBCQgWZprXdaphDhd4c5Xg26NdWEk5MfyhpBHJH83ZE3R1pZ1eeH1xi0JrIMoJ3Gx29Fuvfj-LFzXfxEu5mtGzo0BI_hP3lYmVfwW29Xs76xQj25ET6qxrBwcnpuLoc-fQGXi-yauTXJY5U5xfVl1_7rj1c
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELZKAcGFNyVQwEhwgqiJndjOAaHyqLpqWVWoSL2ZrB90pZIsm91F_VP8RmacZKvl0VsPXBMncZJv5pvxjGcIee6ykjvmZMxKo2Lg4yQuMusAyzbxwOgjZpLQbEIOh-roqDhYIz_7vTCYVtnrxKCobW1wjXwLqEVgJT2Rvpl8j7FrFEZX-xYaLSz23OkPcNma14P38H9fMLbz4fDdbtx1FYiNyPJZ7ETpLCstetvcWzOyHpy0XBlwK60smJeejQwwoQDmVt7kTNpyZBX3hbOAeA73vUQugxnBVEgVPFiu6WDULEuLbm9OwtVWA_yIe9jQR2OCJ7Fc4b_QJuBvtu2fKZq_xWkD_e3c_N8-3C1yozO06XYrGbfJmqvukKtt683Tu-R4UNHFeFHT0bi2WDy46_tFy65KC609nSGRgyDgiiI1nYcxgyGWWudbqqCfhttwp-m8idEisNRNxk39DR69CAGRe-TzhbzmfbJe1ZV7QKgrQfsV3HIvbOakUcpiBXuRG2ESZ9OIpD0WtOlKsmNnkBMdUgO40i1-NOBHB_xoGZGXy2smbUGSc0e_RYgtR2Ix8XCgnn7VnW7SwimpQGBT7nnGwVjJTVpmIrUmzUtlRUQ2e2TpTsM1-gxWEXm2PA2_BANOZeXqeTsGA9kZzGOjxfNyJhwrGcJMIyJXkL4y1dUz1fg41D-XYJQnsojIq14mzqb170_x8Py3eEqu7R5-3Nf7g-HeI3KdochighbfJOuz6dw9JlfMYjZupk-CzFPy5aJl5Re5tpYj
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9QwFLZKWcSFfQkUMBKcIJrETmzngFChVIyKRiMEUm9uxgsdCZJhMjOof41fx3tOMtWw9NYD18RJnOR7m9_z9wh55rKSO-ZkzEqjYrDHSVxk1gGWbeLBok-YSUKzCTkaqcPDYrxFfvZ7YbCssteJQVHb2uAa-QBMi0AmPZEOfFcWMd7bfz37HmMHKcy09u00WogcuJMfEL41r4Z78K-fM7b_7tPb93HXYSA2IssXsROls6y0GHlzb83EegjYcmUgxLSyYF56NjFgFQVYceVNzqQtJ1ZxXzgL6Odw3wvkokTS8lA2OF6v72AGLUuLbp9OwtWgAVuJ-9kwXmOCJ7HcsIWhZcDf_Nw_yzV_y9kGU7h__X_-iDfItc4Bp7utxNwkW666RS63LTlPbpPjYUVX01VNJ9PaIqlw1w-Mlh17C609XaCBBwHBlUZqushjAUMstc63JoR-HO3CnebLJkZPwVI3mzb1N3j0KiRK7pDP5_Kad8l2VVfuPqGuBK1YcMu9sJmTRimLzPYiN8IkzqYRSXtcaNNRtWPHkK86lAxwpVssacCSDljSMiIv1tfMWqKSM0e_QbitRyLJeDhQz7_oTmdp4ZRUIMgp9zzj4MTkJi0B6takeamsiMhOjzLdab5Gn0IsIk_Xp-GXYCKqrFy9bMdggjuDedxrsb2eCUeGQ5hpROQG6jemunmmmh4HXnQJznoii4i87OXjdFr__hQPzn6LJ-QKiIj-MBwdPCRXGUov1m3xHbK9mC_dI3LJrBbTZv44iD8lR-ctKr8ATnme4A
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+vivo+biodistribution+analysis+of+transmission+competent+and+defective+RNA+virus-based+episomal+vector&rft.jtitle=Scientific+reports&rft.au=Komatsu+Yumiko&rft.au=Tanaka+Chiaki&rft.au=Komorizono+Ryo&rft.au=Tomonaga+Keizo&rft.date=2020-04-03&rft.pub=Nature+Publishing+Group&rft.eissn=2045-2322&rft.volume=10&rft.issue=1&rft_id=info:doi/10.1038%2Fs41598-020-62630-7&rft.externalDBID=HAS_PDF_LINK
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon