A Sensitive Method for Determination 1,25-Dihydroxyvitamin D3 in Human Brain using Ultra-Pressure Liquid Chromatography Tandem Mass Spectrometry

The hormonally active form of vitamin D, 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], has been associated with neuroprotective effects in the brain, but has been difficult to measure in human brain tissue because of its low concentration. The aim of this study was to develop and validate a sensitive meth...

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Vydané v:Current developments in nutrition Ročník 8; číslo 8; s. 104418
Hlavní autori: Xuan, Andrew, Dolnikowski, Gregory G, Booth, Sarah L, Shea, M Kyla, Schneider, Julie A, Fu, Xueyan
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Elsevier Inc 01.08.2024
Oxford University Press
American Society for Nutrition
Elsevier
Predmet:
1,25-dihydroxyvitamin D3
brain
Brief Communication: Research Report
Degeneration
Health aspects
Liquid chromatography
Mass spectrometry
Measurement
Nervous system
neurodegenerative diseases
QTRAP
Vitamin D
Vitamin D3
United States
LOD
1,25-dihydroxyvitamin D3
QTRAP
PTAD
RSD
VDR
MT
1,25(OH)2D3
SPE
IS
brain
CR
neurodegenerative diseases
Vitamin D
25(OH)D3
AWS
ISSN:2475-2991, 2475-2991
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Shrnutí:The hormonally active form of vitamin D, 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], has been associated with neuroprotective effects in the brain, but has been difficult to measure in human brain tissue because of its low concentration. The aim of this study was to develop and validate a sensitive method to quantify 1,25(OH)2D3 in the human brain. Prior to analysis by the LC-MS/MS, the samples were derivatized with 4-phenyl-1,2,4-triazoline-3,5-dione. The method showed good linearity of 1,25(OH)2D3 over the physiological range (R2 = 0.9998). The limit of detection was 2.5 pg/g, >10 times lower than the previously reported limit of detection. The average 1,25(OH)2D3 concentrations in 3 regions of human brain tissue samples were: anterior watershed 30.7 pg/g; mid-temporal cortex 19.2 pg/g; and cerebellum 18.5 pg/g. This validated method to quantify 1,25(OH)2D3 in human brain tissue can be applied to obtain information about its presence in various regions of the human brain associated with neurodegenerative diseases.
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ISSN:2475-2991
2475-2991
DOI:10.1016/j.cdnut.2024.104418