A benchmark comparison of CRISPRn guide-RNA design algorithms and generation of small single and dual-targeting libraries to boost screening efficiency

Genome-wide CRISPR sgRNA libraries have emerged as transformative tools to systematically probe gene function. While these libraries have been iterated over time to be more efficient, their large size limits their use in some applications. Here, we benchmarked publicly available genome-wide single-t...

Full description

Saved in:

Bibliographic Details
Published in:	BMC genomics Vol. 26; no. 1; pp. 198 - 9
Main Authors:	Lukasiak, Sebastian, Kalinka, Alex, Gupta, Nikhil, Papadopoulos, Angelos, Saeed, Khalid, McDermott, Ultan, Hannon, Gregory James, Ross-Thriepland, Douglas, Walter, David
Format:	Journal Article
Language:	English
Published:	London BioMed Central 26.02.2025 BioMed Central Ltd Springer Nature B.V BMC
Subjects:	Algorithms Analysis Animal Genetics and Genomics Anopheles Benchmarking Benchmarks Biomedical and Life Sciences Comparative analysis CRISPR CRISPR-Cas Systems CRISPR-Cas9 Efficiency Gene Editing - methods Gene Library Genes Genome-wide Genomes Genomic libraries Genomics Health aspects Humans Life Sciences Methods Microarrays Microbial Genetics and Genomics Plant Genetics and Genomics Pooled CRISPR screening Proteomics RNA RNA, Guide, CRISPR-Cas Systems - genetics SgRNA selection algorithm Austria SgRNA selection algorithm Pooled CRISPR screening Genome-wide CRISPR-Cas9
ISSN:	1471-2164, 1471-2164
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!