Spotted fever rickettsiae in wild-living rodents from south-western Poland

Background Rickettsiae are obligate intracellular alpha-proteobacteria. They are transmitted via arthropod vectors, which transmit the bacteria between animals and occasionally to humans. So far, much research has been conducted to indicate reservoir hosts for these microorganisms, but our knowledge...

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Vydáno v:Parasites & vectors Ročník 10; číslo 1; s. 413
Hlavní autoři: Gajda, Ewa, Hildebrand, Joanna, Sprong, Hein, Buńkowska-Gawlik, Katarzyna, Perec-Matysiak, Agnieszka, Coipan, Elena Claudia
Médium: Journal Article
Jazyk:angličtina
Vydáno: London BioMed Central 06.09.2017
BioMed Central Ltd
BMC
Témata:
Animals
Animals, Wild
Apodemus agrarius
Apodemus flavicollis
arthropods
Biomedical and Life Sciences
Biomedicine
blood
Clethrionomys glareolus
Disease Reservoirs
DNA
DNA, Bacterial - genetics
Entomology
enzootic diseases
fever
genes
Genetic aspects
Health aspects
Infectious Diseases
Murinae - microbiology
Parasitology
pathogens
PCR
Poland
Prevalence
Rickettsia - genetics
Rickettsia - isolation & purification
Rickettsia - physiology
Rickettsia helvetica
Rickettsia spp
Rickettsiae
Rodent Diseases - epidemiology
Rodent Diseases - microbiology
Rodents
sequence analysis
Sequence Analysis, DNA
Short Report
spleen
Spotted Fever Group Rickettsiosis - epidemiology
Spotted Fever Group Rickettsiosis - microbiology
Spotted Fever Group Rickettsiosis - veterinary
surveys
Tropical Medicine
Veterinary Medicine/Veterinary Science
Virology
Poland
Poland
spp
Rodents
PCR
Rickettsia spp
ISSN:1756-3305, 1756-3305
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Shrnutí:Background Rickettsiae are obligate intracellular alpha-proteobacteria. They are transmitted via arthropod vectors, which transmit the bacteria between animals and occasionally to humans. So far, much research has been conducted to indicate reservoir hosts for these microorganisms, but our knowledge is still non-exhaustive. Therefore, this survey was undertaken to investigate the presence of Rickettsia spp. in wild-living small rodents from south-western Poland. Results In total, 337 samples (193 from spleen and 144 from blood) obtained from 193 wild-living rodents: Apodemus agrarius , Apodemus flavicollis , and Myodes glareolus were tested by qPCR for Rickettsia spp. based on a fragment of gltA gene. The prevalence of infection was 17.6% (34/193). Subsequently, the positive samples were analysed by conventional PCR targeting the gltA gene fragment. The genus Rickettsia was confirmed by sequence analysis in four samples from the blood. In two blood samples from A. agrarius , the identified pathogen was Rickettsia helvetica . The Rickettsia obtained from A. flavicollis was assigned to Rickettsia felis -like organisms group. One isolate from A. agrarius could be determined only to the genus level. Conclusions This study shows the presence of Rickettsia DNA in tissues of wild-living rodents, suggesting some potential role of these animals in temporarily maintaining and spreading the bacteria in enzootic cycles.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1756-3305
1756-3305
DOI:10.1186/s13071-017-2356-5