The salivary microbiota of patients with acute lower respiratory tract infection–A multicenter cohort study

The human microbiome contributes to health and disease, but the oral microbiota is understudied relative to the gut microbiota. The salivary microbiota is easily accessible, underexplored, and may provide insight into response to infections. We sought to determine the composition, association with c...

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Bibliographic Details
Published in:PloS one Vol. 19; no. 1; p. e0290062
Main Authors: Rogers, Matthew B., Harner, Ashley, Buhay, Megan, Firek, Brian, Methé, Barbara, Morris, Alison, Palmer, Octavia M. Peck, Promes, Susan B., Sherwin, Robert L., Southerland, Lauren, Vieira, Alexandre R., Yende, Sachin, Morowitz, Michael J., Huang, David T.
Format: Journal Article
Language:English
Published: United States Public Library of Science 11.01.2024
Public Library of Science (PLoS)
Subjects:
Adult
Anaerobes
Analysis
Asthma
Biology and Life Sciences
Care and treatment
Chronic illnesses
Chronic obstructive pulmonary disease
Cohort analysis
Composition
Computer and Information Sciences
Diagnosis
Electronic health records
Emergency medical care
Emergency medical services
Ethylenediaminetetraacetic acid
Feces
Feces - microbiology
Gastrointestinal Microbiome
Geography
Health aspects
Heterogeneity
Hospitalization
Hospitals
Humans
Infection
Infections
Intestinal microflora
Lung diseases, Obstructive
Medical research
Medicine and Health Sciences
Medicine, Experimental
Microbiomes
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Patients
Pneumonia
Prospective Studies
Relative abundance
Respiratory tract
Respiratory tract infection
Respiratory tract infections
Respiratory Tract Infections - microbiology
Review boards
RNA
RNA, Ribosomal, 16S - genetics
rRNA
Text messaging
Ventilators
United States
Michigan
Pennsylvania
ISSN:1932-6203, 1932-6203
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Summary:The human microbiome contributes to health and disease, but the oral microbiota is understudied relative to the gut microbiota. The salivary microbiota is easily accessible, underexplored, and may provide insight into response to infections. We sought to determine the composition, association with clinical features, and heterogeneity of the salivary microbiota in patients with acute lower respiratory tract infection (LRTI). We conducted a multicenter prospective cohort study of 147 adults with acute LRTI presenting to the emergency department of seven hospitals in three states (Pennsylvania, Michigan, and Ohio) between May 2017 and November 2018. Salivary samples were collected in the emergency department, at days 2–5 if hospitalized, and at day 30, as well as fecal samples if patients were willing. We compared salivary microbiota profiles from patients to those of healthy adult volunteers by sequencing and analyzing bacterial 16-rRNA. Compared to healthy volunteers, the salivary microbiota of patients with LRTI was highly distinct and strongly enriched with intestinal anaerobes such as Bacteroidaceae , Ruminococcaceae , and Lachnospiraceae (e.g., mean 10% relative abundance of Bacteroides vs < 1% in healthy volunteers). Within the LRTI population, COPD exacerbation was associated with altered salivary microbiota composition compared to other LRTI conditions. The largest determinant of microbiota variation within the LRTI population was geography (city in which the hospital was located).
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0290062