Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay

Mosquitoes are major infectious disease-carrying vectors. Assessment of current and future risks associated with the mosquito population requires knowledge of the full repertoire of pathogens they carry, including novel viruses, as well as their blood meal sources. Unbiased metatranscriptomic sequen...

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Vydáno v:eLife Ročník 10
Hlavní autoři: Batson, Joshua, Dudas, Gytis, Haas-Stapleton, Eric, Kistler, Amy L, Li, Lucy M, Logan, Phoenix, Ratnasiri, Kalani, Retallack, Hanna
Médium: Journal Article
Jazyk:angličtina
Vydáno: England eLife Science Publications, Ltd 27.04.2021
eLife Sciences Publications Ltd
eLife Sciences Publications, Ltd
Témata:
Aedes - genetics
Animals
blood meal reservoir
California
Communicable Diseases, Emerging - microbiology
Communicable Diseases, Emerging - transmission
Communicable Diseases, Emerging - virology
Culex - genetics
Culicidae
Culicidae - genetics
Culicidae - microbiology
Culicidae - virology
Dengue fever
Disease Reservoirs - microbiology
Disease Reservoirs - virology
Epidemiology
Epidemiology and Global Health
Exome Sequencing - methods
Gene Expression Profiling - methods
Genetics and Genomics
Genomes
Health aspects
Infections
Infectious diseases
Insect control
Insect Vectors - genetics
Insect Vectors - microbiology
Insect Vectors - virology
Microbiota
Morphology
mosquito
Mosquitoes
Pathogenic microorganisms
Pathogens
Prokaryotes
Public health
Tools and Resources
Vectors
viral discovery
Viruses
West Nile virus
xenosurveillance
California
Canada
China
United States > US
global health
microbiota
epidemiology
genetics
xenosurveillance
genomics
viral discovery
blood meal reservoir
virus
mosquito
ISSN:2050-084X, 2050-084X
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Shrnutí:Mosquitoes are major infectious disease-carrying vectors. Assessment of current and future risks associated with the mosquito population requires knowledge of the full repertoire of pathogens they carry, including novel viruses, as well as their blood meal sources. Unbiased metatranscriptomic sequencing of individual mosquitoes offers a straightforward, rapid, and quantitative means to acquire this information. Here, we profile 148 diverse wild-caught mosquitoes collected in California and detect sequences from eukaryotes, prokaryotes, 24 known and 46 novel viral species. Importantly, sequencing individuals greatly enhanced the value of the biological information obtained. It allowed us to (a) speciate host mosquito, (b) compute the prevalence of each microbe and recognize a high frequency of viral co-infections, (c) associate animal pathogens with specific blood meal sources, and (d) apply simple co-occurrence methods to recover previously undetected components of highly prevalent segmented viruses. In the context of emerging diseases, where knowledge about vectors, pathogens, and reservoirs is lacking, the approaches described here can provide actionable information for public health surveillance and intervention decisions.
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These authors contributed equally to this work.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.68353