Interaction of Tyrosine-Based Sorting Signals with Clathrin-Associated Proteins

Tyrosine-based signals within the cytoplasmic domain of integral membrane proteins mediate clathrin-dependent protein sorting in the endocytic and secretory pathways. A yeast two-hybrid system was used to identify proteins that bind to tyrosine-based signals. The medium chains ($\mu_1$ and $\mu_2$)...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) Jg. 269; H. 5232; S. 1872 - 1875
Hauptverfasser:	Ohno, Hiroshi, Stewart, Jay, Fournier, Marie-Christine, Bosshart, Herbert, Rhee, Ina, Miyatake, Shoichiro, Saito, Takashi, Gallusser, Andreas, Kirchhausen, Tomas, Bonifacino, Juan S.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Washington, DC American Society for the Advancement of Science 29.09.1995 American Association for the Advancement of Science The American Association for the Advancement of Science
Schlagworte:	Adaptor Proteins, Vesicular Transport Amino Acid Sequence Amino acids Biological and medical sciences Cell Membrane - metabolism Cell physiology Cellular signal transduction Clathrin - metabolism Cloning, Molecular Complementary DNA Epiphyses Fundamental and applied biological sciences. Psychology Genetic mutation Glutathione Transferase - metabolism Golgi Apparatus - metabolism Goods and services tax Internalization Libraries Literary Devices Lysosomes - metabolism Membrane and intracellular transports Membrane proteins Membrane Proteins - chemistry Membrane Proteins - metabolism Membranes Molecular and cellular biology Molecular Sequence Data Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - metabolism Observation Phosphoproteins - chemistry Phosphoproteins - metabolism Plasmids Polymerase chain reaction Protein Sorting Signals - chemistry Protein Sorting Signals - metabolism Proteins Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Transformation, Genetic Tyrosine - metabolism Yeast Yeasts Tyrosine Leader peptide Intracellular transport Clathrin Membrane protein Yeast Targeting Molecular interaction Molecular targeting Sorting Fungi Ascomycetes Saccharomyces cerevisiae Thallophyta
ISSN:	0036-8075, 1095-9203
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Zusammenfassung:	Tyrosine-based signals within the cytoplasmic domain of integral membrane proteins mediate clathrin-dependent protein sorting in the endocytic and secretory pathways. A yeast two-hybrid system was used to identify proteins that bind to tyrosine-based signals. The medium chains ($\mu_1$ and $\mu_2$) of two clathrin-associated protein complexes (AP-1 and AP-2, respectively) specifically interacted with tyrosine-based signals of several integral membrane proteins. The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery.
Bibliographie:	SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-2 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.7569928