Yeast nutrient transceptors provide novel insight in the functionality of membrane transporters

In the yeast Saccharomyces cerevisiae several nutrient transporters have been identified that possess an additional function as nutrient receptor. These transporters are induced when yeast cells are starved for their substrate, which triggers entry into stationary phase and acquirement of a low prot...

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Bibliographic Details
Published in:Current genetics Vol. 59; no. 4; pp. 197 - 206
Main Authors: Schothorst, Joep, Kankipati, Harish Nag, Conrad, Michaela, Samyn, Dieter R, Van Zeebroeck, Griet, Popova, Yulia, Rubio-Texeira, Marta, Persson, Bengt L, Thevelein, Johan M
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer-Verlag 01.11.2013
Springer Berlin Heidelberg
Springer Nature B.V
Subjects:
agonists
Amino Acid Sequence
Amino Acid Transport Systems
Amino Acid Transport Systems - metabolism
Amino acids
Biochemistry
Biokemi
Biomedical and Life Sciences
cAMP-dependent protein kinase
Cell Biology
chemistry
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP-Dependent Protein Kinases - metabolism
endocytosis
Endocytosis - physiology
genetics
Inactivation
ions
Life Sciences
Membrane Transport Proteins
Membrane Transport Proteins - chemistry
Membrane Transport Proteins - metabolism
metabolism
Microbial Genetics and Genomics
Microbiology
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Nutrients
phenotype
Phosphates
Phosphates - chemistry
Phosphates - metabolism
physiology
Plant Sciences
Protein Conformation
Proteomics
Research Article
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae Proteins - metabolism
Signal Transduction
Signal Transduction - physiology
site-directed mutagenesis
Sodium
Sodium - metabolism
symporters
Ubiquitination
vacuoles
Yeasts
Endocytosis
Ubiquitination
Proton binding
Nutrient signaling
Co-transport
Transceptor
ISSN:0172-8083, 1432-0983, 1432-0983
Online Access:Get full text
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Description
Summary:In the yeast Saccharomyces cerevisiae several nutrient transporters have been identified that possess an additional function as nutrient receptor. These transporters are induced when yeast cells are starved for their substrate, which triggers entry into stationary phase and acquirement of a low protein kinase A (PKA) phenotype. Re-addition of the lacking nutrient triggers exit from stationary phase and sudden activation of the PKA pathway, the latter being mediated by the nutrient transceptors. At the same time, the transceptors are ubiquitinated, endocytosed and sorted to the vacuole for breakdown. Investigation of the signaling function of the transceptors has provided a new read-out and new tools for gaining insight into the functionality of transporters. Identification of amino acid residues that bind co-transported ions in symporters has been challenging because the inactivation of transport by site-directed mutagenesis is not conclusive with respect to the cause of the inactivation. The discovery of nontransported agonists of the signaling function in transceptors has shown that transport is not required for signaling. Inactivation of transport with maintenance of signaling in transceptors supports that a true proton-binding residue was mutagenised. Determining the relationship between transport and induction of endocytosis has also been challenging, since inactivation of transport by mutagenesis easily causes loss of all affinity for the substrate. The use of analogues with different combinations of transport and signaling capacities has revealed that transport, ubiquitination and endocytosis can be uncoupled in several unexpected ways. The results obtained are consistent with transporters undergoing multiple substrate-induced conformational changes, which allow interaction with different accessory proteins to trigger specific downstream events.
Bibliography:http://dx.doi.org/10.1007/s00294-013-0413-y
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Communicated by H. Sychrova.
ISSN:0172-8083
1432-0983
1432-0983
DOI:10.1007/s00294-013-0413-y