Silk based bioinks for soft tissue reconstruction using 3-dimensional (3D) printing with in vitro and in vivo assessments

In the field of soft tissue reconstruction, custom implants could address the need for materials that can fill complex geometries. Our aim was to develop a material system with optimal rheology for material extrusion, that can be processed in physiological and non-toxic conditions and provide struct...

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Vydáno v:Biomaterials Ročník 117; s. 105 - 115
Hlavní autoři: Rodriguez, María J., Brown, Joseph, Giordano, Jodie, Lin, Samuel J., Omenetto, Fiorenzo G., Kaplan, David L.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Netherlands Elsevier Ltd 01.02.2017
Témata:
3D printing
Advanced Basic Science
Animals
Biocompatible Materials - chemical synthesis
Bioink
Compressive Strength
Connective Tissue - growth & development
Connective Tissue Cells - physiology
crosslinking
Dentistry
Elastic Modulus
extrusion
gelatin
Gelatin - chemistry
glycerol
Guided Tissue Regeneration - instrumentation
Guided Tissue Regeneration - methods
Ink
Materials Testing
mechanical properties
Mice
Mice, Inbred BALB C
Printing, Three-Dimensional
Regeneration
rheology
Silk
Silk - chemistry
Silk - ultrastructure
Soft tissue
Tissue Scaffolds
Viscosity
Regeneration
Silk
Soft tissue
Bioink
3D printing
ISSN:0142-9612, 1878-5905
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Popis
Shrnutí:In the field of soft tissue reconstruction, custom implants could address the need for materials that can fill complex geometries. Our aim was to develop a material system with optimal rheology for material extrusion, that can be processed in physiological and non-toxic conditions and provide structural support for soft tissue reconstruction. To meet this need we developed silk based bioinks using gelatin as a bulking agent and glycerol as a non-toxic additive to induce physical crosslinking. We developed these inks optimizing printing efficacy and resolution for patient-specific geometries that can be used for soft tissue reconstruction. We demonstrated in vitro that the material was stable under physiological conditions and could be tuned to match soft tissue mechanical properties. We demonstrated in vivo that the material was biocompatible and could be tuned to maintain shape and volume up to three months while promoting cellular infiltration and tissue integration.
Bibliografie:ObjectType-Article-1
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ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2016.11.046