Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells

Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require samples comprising a minimum of thousands of cells to provide in-depth profiling. Here, we report the development of a nanoPOTS (nanodroplet proc...

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Vydáno v:	Nature communications Ročník 9; číslo 1; s. 882 - 10
Hlavní autoři:	Zhu, Ying, Piehowski, Paul D., Zhao, Rui, Chen, Jing, Shen, Yufeng, Moore, Ronald J., Shukla, Anil K., Petyuk, Vladislav A., Campbell-Thompson, Martha, Mathews, Clayton E., Smith, Richard D., Qian, Wei-Jun, Kelly, Ryan T.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 28.02.2018 Nature Publishing Group Nature Portfolio
Témata:	631/1647/2067 631/1647/296 82/16 82/58 82/62 82/80 BASIC BIOLOGICAL SCIENCES Biomedical materials Depth profiling Diabetes mellitus Gene Expression Profiling Humanities and Social Sciences Humans Islets of Langerhans - chemistry Islets of Langerhans - metabolism Liquid chromatography Mammalian cells Mass Spectrometry Mass spectroscopy multidisciplinary Pancreas Proteins Proteins - chemistry Proteins - genetics Proteins - metabolism Proteome - chemistry Proteome - genetics Proteome - metabolism Proteomics Science Science (multidisciplinary)
ISSN:	2041-1723, 2041-1723
On-line přístup:	Získat plný text
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