Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

GCaMP, one popular type of genetically-encoded Ca 2+ indicator, has been associated with various side-effects. Here we unveil the intrinsic problem prevailing over different versions and applications, showing that GCaMP containing CaM (calmodulin) interferes with both gating and signaling of L-type...

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Bibliographic Details
Published in:	Nature communications Vol. 9; no. 1; pp. 1504 - 18
Main Authors:	Yang, Yaxiong, Liu, Nan, He, Yuanyuan, Liu, Yuxia, Ge, Lin, Zou, Linzhi, Song, Sen, Xiong, Wei, Liu, Xiaodong
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 17.04.2018 Nature Publishing Group Nature Portfolio
Subjects:	14/1 14/19 14/34 14/35 14/63 42/109 631/1647/245/2225 631/378/2586 631/45/269/1146 631/80/86/1999 631/80/86/2372 9/10 9/74 96/95 Animals Biosensing Techniques - methods Calcium - chemistry Calcium - metabolism Calcium channels Calcium channels (L-type) Calcium channels (voltage-gated) Calcium Channels, L-Type - metabolism Calcium imaging Calcium ions Calcium Signaling Calcium signalling Calcium-binding protein Calmodulin Calmodulin - chemistry Calmodulin - genetics Calmodulin - metabolism Channel gating Coupling (molecular) Fluorescent Dyes - chemistry Gene expression Genetic Engineering - methods Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics HEK293 Cells Humanities and Social Sciences Humans Intravital Microscopy - methods Ion Channel Gating Mice Mice, Inbred ICR Molecular Imaging - methods Morphogenesis multidisciplinary Neurons Patch-Clamp Techniques Primary Cell Culture Protein Binding Science Science (multidisciplinary) Side effects Transcription
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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