Plasma serotonin levels and the platelet serotonin transporter

Serotonin (5HT) is a platelet-stored vasoconstrictor. Altered concentrations of circulating 5HT are implicated in several pathologic conditions, including hypertension. The actions of 5HT are mediated by different types of receptors and terminated by a single 5HT transporter (SERT). Therefore, SERT...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Journal of neurochemistry Ročník 102; číslo 1; s. 206 - 215
Hlavní autoři:	Brenner, B, Harney, J.T, Ahmed, B.A, Jeffus, B.C, Unal, R, Mehta, J.L, Kilic, F
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.07.2007 Blackwell Publishing Ltd Blackwell
Témata:	Arterial hypertension. Arterial hypotension Biological and medical sciences Biophysics biosynthesis Biotin blood Blood and lymphatic vessels Blood Platelets Blood Platelets - metabolism Blood pressure Blotting, Western Cardiology. Vascular system Cell Membrane Cell Membrane - drug effects Cell Membrane - metabolism Cell receptors Cell structures and functions drug effects Enzyme-Linked Immunosorbent Assay Feedback Feedback - physiology Fundamental and applied biological sciences. Psychology Humans Hypertension Hypertension - blood Hypotheses Kinetics Male Medical sciences metabolism Middle Aged Molecular and cellular biology Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) Neurotransmitters physiology Plasma plasma serotonin level platelet serotonin uptake serotonin Serotonin - blood Serotonin Plasma Membrane Transport Proteins Serotonin Plasma Membrane Transport Proteins - biosynthesis Serotonin Plasma Membrane Transport Proteins - blood serotonin transporter Signal transduction Hypertension Serotonin Vasoconstriction Cardiovascular disease platelet serotonin uptake Uptake Blood Vasomotricity Chronic Platelet Serotonin transporter Plasma membrane Neurotransmitter Models plasma serotonin level Biological receptor
ISSN:	0022-3042, 1471-4159
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Serotonin (5HT) is a platelet-stored vasoconstrictor. Altered concentrations of circulating 5HT are implicated in several pathologic conditions, including hypertension. The actions of 5HT are mediated by different types of receptors and terminated by a single 5HT transporter (SERT). Therefore, SERT is a major mechanism that regulates plasma 5HT levels to prevent vasoconstriction and thereby secure a stable blood flow. In this study, the response of platelet SERT to the plasma 5HT levels was examined within two models: (i) in subjects with chronic hypertension or normotension; (ii) on platelets isolated from normotensive subjects and pretreated with 5HT at various concentrations. The platelet 5HT uptake rates were lower during hypertension due to a decrease in Vmax with a similar Km; also, the decrease in Vmax was primarily due to a decrease in the density of SERT on the platelet membrane, with no change in whole cell expression. Additionally, while the platelet 5HT content decreased 33%, the plasma 5HT content increased 33%. Furthermore, exogenous 5HT altered the 5HT uptake rates by changing the density of SERT molecules on the plasma membrane in a biphasic manner. Therefore, we hypothesize that in a hypertensive state, the elevated plasma 5HT levels induces a loss in 5HT uptake function in platelets via a decrease in the density of SERT molecules on the plasma membrane. Through the feedback effect of this proposed mechanism, plasma 5HT controls its own concentration levels by modulating the uptake properties of platelet SERT.
Bibliografie:	http://dx.doi.org/10.1111/j.1471-4159.2007.04542.x ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0022-3042 1471-4159
DOI:	10.1111/j.1471-4159.2007.04542.x