Effectiveness of 222-nm ultraviolet light on disinfecting SARS-CoV-2 surface contamination

•222-nm UVC light (0.1 mW/cm2) reduced viable SARS-CoV-2 by 0.94 log10 in 10 seconds.•222-nm UVC light (0.1 mW/cm2) reduced viable SARS-CoV-2 by 2.51 log10 in 30 seconds.•222-nm UVC light did not reduce SARS-CoV-2 RNA copy number after 5-minute irradiation.•TSID50 and not RT-qPCR should be used to m...

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Vydané v:American journal of infection control Ročník 49; číslo 3; s. 299 - 301
Hlavní autori: Kitagawa, Hiroki, Nomura, Toshihito, Nazmul, Tanuza, Omori, Keitaro, Shigemoto, Norifumi, Sakaguchi, Takemasa, Ohge, Hiroki
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Elsevier Inc 01.03.2021
Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc
Predmet:
COVID-19
COVID-19 - prevention & control
COVID-19 - virology
Decontamination - methods
Disinfection
Disinfection - methods
Environmental contamination
Far-UVC
Humans
Infectious Disease
Major
RNA, Viral - radiation effects
SARS-CoV-2 - radiation effects
Ultraviolet Rays
COVID-19
Disinfection
Environmental contamination
Far-UVC
ISSN:0196-6553, 1527-3296, 1527-3296
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Shrnutí:•222-nm UVC light (0.1 mW/cm2) reduced viable SARS-CoV-2 by 0.94 log10 in 10 seconds.•222-nm UVC light (0.1 mW/cm2) reduced viable SARS-CoV-2 by 2.51 log10 in 30 seconds.•222-nm UVC light did not reduce SARS-CoV-2 RNA copy number after 5-minute irradiation.•TSID50 and not RT-qPCR should be used to monitor far-UVC disinfection of surfaces. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), has emerged as a serious threat to human health worldwide. Efficient disinfection of surfaces contaminated with SARS-CoV-2 may help prevent its spread. This study aimed to investigate the in vitro efficacy of 222-nm far-ultraviolet light (UVC) on the disinfection of SARS-CoV-2 surface contamination. We investigated the titer of SARS-CoV-2 after UV irradiation (0.1 mW/cm2) at 222 nm for 10-300 seconds using the 50% tissue culture infectious dose (TCID50). In addition, we used quantitative reverse transcription polymerase chain reaction to quantify SARS-CoV-2 RNA under the same conditions. One and 3 mJ/cm2 of 222-nm UVC irradiation (0.1 mW/cm2 for 10 and 30 seconds) resulted in 88.5 and 99.7% reduction of viable SARS-CoV-2 based on the TCID50 assay, respectively. In contrast, the copy number of SARS-CoV-2 RNA did not change after UVC irradiation even after a 5-minute irradiation. This study shows the efficacy of 222-nm UVC irradiation against SARS-CoV-2 contamination in an in vitro experiment. Further evaluation of the safety and efficacy of 222-nm UVC irradiation in reducing the contamination of real-world surfaces and the potential transmission of SARS-CoV-2 is needed.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0196-6553
1527-3296
1527-3296
DOI:10.1016/j.ajic.2020.08.022