Construction and Characterization of Virus-Like Particles: A Review

Over the last three decades, virus-like particles (VLPs) have evolved to become a widely accepted technology, especially in the field of vaccinology. In fact, some VLP-based vaccines are currently used as commercial medical products, and other VLP-based products are at different stages of clinical s...

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Bibliographic Details
Published in:Molecular biotechnology Vol. 53; no. 1; pp. 92 - 107
Main Author: Zeltins, Andris
Format: Journal Article
Language:English
Published: New York Springer-Verlag 01.01.2013
Humana Press Inc
Springer Nature B.V
Subjects:
Animals
Biochemistry
Biological Techniques
Biotechnology
Cell Biology
Cell-Free System
Cell-Free System - metabolism
Chemistry
Chemistry and Materials Science
Cloning
Cloning, Molecular
Data processing
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Gene therapy
Genes, Viral
Genetic Therapy
Genetic Vectors
genetics
Host systems
Human Genetics
Humans
immunology
Insecta
Insecta - genetics
Insecta - metabolism
Medical equipment
metabolism
Nanomaterials
Nanoparticles
Nanotechnology
Nucleic acids
Plants
Plants - genetics
Plants - metabolism
Protein Science
Purification
Recombinant Proteins
Recombinant Proteins - chemistry
Review
Reviews
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
structural genes
Structural integrity
Vaccines
Vaccines, Virus-Like Particle
Vaccines, Virus-Like Particle - chemistry
Vaccines, Virus-Like Particle - immunology
Viral Vaccines
Viral Vaccines - chemistry
Viral Vaccines - immunology
Virology
Virus-like particles
Viruses
Viruses - genetics
Viruses - immunology
Virus
Expression system
Self-assembly
Vaccine
Nanoparticle
Cloning
ISSN:1073-6085, 1559-0305, 1559-0305
Online Access:Get full text
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Summary:Over the last three decades, virus-like particles (VLPs) have evolved to become a widely accepted technology, especially in the field of vaccinology. In fact, some VLP-based vaccines are currently used as commercial medical products, and other VLP-based products are at different stages of clinical study. Several remarkable advantages have been achieved in the development of VLPs as gene therapy tools and new nanomaterials. The analysis of published data reveals that at least 110 VLPs have been constructed from viruses belonging to 35 different families. This review therefore discusses the main principles in the cloning of viral structural genes, the relevant host systems and the purification procedures that have been developed. In addition, the methods that are used to characterize the structural integrity, stability, and components, including the encapsidated nucleic acids, of newly synthesized VLPs are analyzed. Moreover, some of the modifications that are required to construct VLP-based carriers of viral origin with defined properties are discussed, and examples are provided.
Bibliography:http://dx.doi.org/10.1007/s12033-012-9598-4
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ISSN:1073-6085
1559-0305
1559-0305
DOI:10.1007/s12033-012-9598-4