Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome

A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed. The assay was validated with sera collected from a CHIKV outbreak in Senegal in 20...

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Bibliographic Details
Published in:	PLoS neglected tropical diseases Vol. 12; no. 5; p. e0006448
Main Authors:	Lopez-Jimena, Benjamin, Wehner, Stefanie, Harold, Graham, Bakheit, Mohammed, Frischmann, Sieghard, Bekaert, Michaël, Faye, Oumar, Sall, Amadou Alpha, Weidmann, Manfred
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 29.05.2018 Public Library of Science (PLoS)
Subjects:	Aquaculture Assaying Bioassay Bioinformatics Biology and life sciences Chikungunya fever Chikungunya Fever - diagnosis Chikungunya Fever - virology Chikungunya virus Chikungunya virus - classification Chikungunya virus - genetics Chikungunya virus - isolation & purification Dengue fever Detection Diagnosis DNA DNA Primers - genetics Genetic aspects Genome, Viral Genomes Genomics Humans Infections Laboratories Lava Medicine and Health Sciences Methods Molecular chains Molecules Nucleic Acid Amplification Techniques - methods Nucleic acids Nucleotide sequence Oligonucleotides Outbreaks PCR Phylogeny Physical sciences Polymerase chain reaction Principal components analysis Quality assessment Quality control Reproducibility Research and analysis methods Reverse transcriptase Reverse transcription Ribonucleic acid RNA Sensitivity and Specificity Software Supervision Transcription Tropical diseases Vector-borne diseases Viral diseases Viroses Viruses Senegal Scotland United Kingdom > UK Germany
ISSN:	1935-2735, 1935-2727, 1935-2735
Online Access:	Get full text
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