Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity

Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid infection utilizing small CRISPR RNAs that are complementary to the invader's genome and specify the targets of RNA-guided Cas nucleases. Ty...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Cell Ročník 161; číslo 5; s. 1164
Hlavní autoři: Samai, Poulami, Pyenson, Nora, Jiang, Wenyan, Goldberg, Gregory W, Hatoum-Aslan, Asma, Marraffini, Luciano A
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 21.05.2015
Témata:
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Cas Systems
DNA - genetics
DNA - metabolism
Ribonucleoproteins - metabolism
RNA - genetics
RNA - metabolism
Staphylococcus epidermidis - immunology
Staphylococcus epidermidis - metabolism
Staphylococcus epidermidis - virology
Transcription, Genetic
ISSN:1097-4172, 1097-4172
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid infection utilizing small CRISPR RNAs that are complementary to the invader's genome and specify the targets of RNA-guided Cas nucleases. Type III CRISPR-Cas immunity requires target transcription, and whereas genetic studies demonstrated DNA targeting, in vitro data have shown crRNA-guided RNA cleavage. The molecular mechanism behind these disparate activities is not known. Here, we show that transcription across the targets of the Staphylococcus epidermidis type III-A CRISPR-Cas system results in the cleavage of the target DNA and its transcripts, mediated by independent active sites within the Cas10-Csm ribonucleoprotein effector complex. Immunity against plasmids and DNA viruses requires DNA, but not RNA, cleavage activity. Our studies reveal a highly versatile mechanism of CRISPR immunity that can defend microorganisms against diverse DNA and RNA invaders.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1097-4172
1097-4172
DOI:10.1016/j.cell.2015.04.027