Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity

Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid infection utilizing small CRISPR RNAs that are complementary to the invader's genome and specify the targets of RNA-guided Cas nucleases. Ty...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Cell Ročník 161; číslo 5; s. 1164
Hlavní autoři: Samai, Poulami, Pyenson, Nora, Jiang, Wenyan, Goldberg, Gregory W, Hatoum-Aslan, Asma, Marraffini, Luciano A
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 21.05.2015
Témata:
ISSN:1097-4172, 1097-4172
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Abstract Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid infection utilizing small CRISPR RNAs that are complementary to the invader's genome and specify the targets of RNA-guided Cas nucleases. Type III CRISPR-Cas immunity requires target transcription, and whereas genetic studies demonstrated DNA targeting, in vitro data have shown crRNA-guided RNA cleavage. The molecular mechanism behind these disparate activities is not known. Here, we show that transcription across the targets of the Staphylococcus epidermidis type III-A CRISPR-Cas system results in the cleavage of the target DNA and its transcripts, mediated by independent active sites within the Cas10-Csm ribonucleoprotein effector complex. Immunity against plasmids and DNA viruses requires DNA, but not RNA, cleavage activity. Our studies reveal a highly versatile mechanism of CRISPR immunity that can defend microorganisms against diverse DNA and RNA invaders.
AbstractList Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid infection utilizing small CRISPR RNAs that are complementary to the invader's genome and specify the targets of RNA-guided Cas nucleases. Type III CRISPR-Cas immunity requires target transcription, and whereas genetic studies demonstrated DNA targeting, in vitro data have shown crRNA-guided RNA cleavage. The molecular mechanism behind these disparate activities is not known. Here, we show that transcription across the targets of the Staphylococcus epidermidis type III-A CRISPR-Cas system results in the cleavage of the target DNA and its transcripts, mediated by independent active sites within the Cas10-Csm ribonucleoprotein effector complex. Immunity against plasmids and DNA viruses requires DNA, but not RNA, cleavage activity. Our studies reveal a highly versatile mechanism of CRISPR immunity that can defend microorganisms against diverse DNA and RNA invaders.
Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid infection utilizing small CRISPR RNAs that are complementary to the invader's genome and specify the targets of RNA-guided Cas nucleases. Type III CRISPR-Cas immunity requires target transcription, and whereas genetic studies demonstrated DNA targeting, in vitro data have shown crRNA-guided RNA cleavage. The molecular mechanism behind these disparate activities is not known. Here, we show that transcription across the targets of the Staphylococcus epidermidis type III-A CRISPR-Cas system results in the cleavage of the target DNA and its transcripts, mediated by independent active sites within the Cas10-Csm ribonucleoprotein effector complex. Immunity against plasmids and DNA viruses requires DNA, but not RNA, cleavage activity. Our studies reveal a highly versatile mechanism of CRISPR immunity that can defend microorganisms against diverse DNA and RNA invaders.Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid infection utilizing small CRISPR RNAs that are complementary to the invader's genome and specify the targets of RNA-guided Cas nucleases. Type III CRISPR-Cas immunity requires target transcription, and whereas genetic studies demonstrated DNA targeting, in vitro data have shown crRNA-guided RNA cleavage. The molecular mechanism behind these disparate activities is not known. Here, we show that transcription across the targets of the Staphylococcus epidermidis type III-A CRISPR-Cas system results in the cleavage of the target DNA and its transcripts, mediated by independent active sites within the Cas10-Csm ribonucleoprotein effector complex. Immunity against plasmids and DNA viruses requires DNA, but not RNA, cleavage activity. Our studies reveal a highly versatile mechanism of CRISPR immunity that can defend microorganisms against diverse DNA and RNA invaders.
Author Pyenson, Nora
Marraffini, Luciano A
Jiang, Wenyan
Goldberg, Gregory W
Samai, Poulami
Hatoum-Aslan, Asma
Author_xml – sequence: 1
  givenname: Poulami
  surname: Samai
  fullname: Samai, Poulami
  organization: Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
– sequence: 2
  givenname: Nora
  surname: Pyenson
  fullname: Pyenson, Nora
  organization: Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
– sequence: 3
  givenname: Wenyan
  surname: Jiang
  fullname: Jiang, Wenyan
  organization: Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
– sequence: 4
  givenname: Gregory W
  surname: Goldberg
  fullname: Goldberg, Gregory W
  organization: Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
– sequence: 5
  givenname: Asma
  surname: Hatoum-Aslan
  fullname: Hatoum-Aslan, Asma
  organization: Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
– sequence: 6
  givenname: Luciano A
  surname: Marraffini
  fullname: Marraffini, Luciano A
  email: marraffini@rockefeller.edu
  organization: Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. Electronic address: marraffini@rockefeller.edu
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25959775$$D View this record in MEDLINE/PubMed
BookMark eNpNkMtOwzAURC1URB_wAyyQl2wSrp04jpdVeEVUgEpZR9exW6VKnBAnSP17igCJ1ZnFaDQ6czJxrbOEXDIIGbDkZh-Wtq5DDkyEEIfA5QmZMVAyiJnkk395Sube7wEgFUKckSkXSigpxYw8ZW0w9Oh82VfdULUOa3r7vKToDF0fmdUWP3FnqRn7yu3o5tBZmuc5zdb52-s6yNDTvGlGVw2Hc3K6xdrbi18uyPv93SZ7DFYvD3m2XAVlwuMhECJWrJRGWR5poXHLInE8yQwkWmtj0ojHClmJyiIIYaBEbeNIcpYqLeOUL8j1z27Xtx-j9UPRVP5bBTrbjr5gSRqBTBSwY_Xqtzrqxpqi66sG-0PxJ4B_AcTrXYM
CitedBy_id crossref_primary_10_1134_S0006297916070026
crossref_primary_10_1139_cjm_2020_0212
crossref_primary_10_1007_s00792_016_0871_5
crossref_primary_10_3390_plants10030532
crossref_primary_10_1146_annurev_virology_100120_010228
crossref_primary_10_1016_j_chom_2019_09_002
crossref_primary_10_1146_annurev_food_072816_024723
crossref_primary_10_1080_14737159_2021_1922080
crossref_primary_10_1016_j_molcel_2016_11_031
crossref_primary_10_1093_nar_gkx150
crossref_primary_10_1101_gad_273722_115
crossref_primary_10_1093_nar_gkaf541
crossref_primary_10_3389_fmicb_2023_1060337
crossref_primary_10_7554_eLife_81897
crossref_primary_10_1002_advs_202506049
crossref_primary_10_1016_j_jbc_2024_107295
crossref_primary_10_15252_embr_201643700
crossref_primary_10_1016_j_mib_2017_05_008
crossref_primary_10_1038_nature23532
crossref_primary_10_1093_nar_gkx726
crossref_primary_10_1128_spectrum_01277_23
crossref_primary_10_1016_j_micres_2022_127100
crossref_primary_10_1016_j_chom_2025_07_021
crossref_primary_10_1093_biomethods_bpy002
crossref_primary_10_3390_v10060335
crossref_primary_10_1002_VIW_20200008
crossref_primary_10_1016_j_ijbiomac_2020_03_043
crossref_primary_10_1038_nature19802
crossref_primary_10_1016_j_molcel_2016_03_024
crossref_primary_10_1039_D5CC03257A
crossref_primary_10_1016_j_sbi_2017_10_015
crossref_primary_10_1038_nri3910
crossref_primary_10_1016_j_heliyon_2024_e34333
crossref_primary_10_1016_j_tibtech_2016_02_004
crossref_primary_10_1093_nar_gkab073
crossref_primary_10_1093_nar_gkae462
crossref_primary_10_3389_fcimb_2020_619763
crossref_primary_10_1042_BSR20170788
crossref_primary_10_1093_nar_gkac923
crossref_primary_10_1016_j_chom_2021_03_018
crossref_primary_10_1016_j_cej_2025_165777
crossref_primary_10_1038_s41577_025_01206_w
crossref_primary_10_1002_bit_25851
crossref_primary_10_1007_s12668_023_01245_4
crossref_primary_10_3389_fmicb_2020_01937
crossref_primary_10_3390_ijms24108623
crossref_primary_10_1016_j_molcel_2024_12_007
crossref_primary_10_1093_nar_gkz676
crossref_primary_10_3389_fmicb_2021_736565
crossref_primary_10_1261_rna_054098_115
crossref_primary_10_1093_nar_gkae676
crossref_primary_10_1016_j_molcel_2015_05_023
crossref_primary_10_1093_nar_gkad101
crossref_primary_10_3389_fmicb_2023_1289683
crossref_primary_10_1016_j_cell_2015_05_003
crossref_primary_10_1016_j_cej_2025_166510
crossref_primary_10_1093_nar_gkaa634
crossref_primary_10_1038_s41589_020_00700_7
crossref_primary_10_7554_eLife_81678
crossref_primary_10_1093_nar_gkv1140
crossref_primary_10_1016_j_engmic_2023_100102
crossref_primary_10_1371_journal_pone_0287461
crossref_primary_10_1016_j_engmic_2023_100101
crossref_primary_10_3389_fmicb_2019_03078
crossref_primary_10_1093_nar_gkv882
crossref_primary_10_1093_nar_gkae1006
crossref_primary_10_1126_science_aaq0179
crossref_primary_10_1016_j_tig_2019_03_009
crossref_primary_10_1016_j_virol_2024_110209
crossref_primary_10_1111_febs_15139
crossref_primary_10_1016_j_bbrc_2019_12_046
crossref_primary_10_1016_j_molcel_2020_04_011
crossref_primary_10_1126_science_aao0100
crossref_primary_10_1016_j_micpath_2019_103551
crossref_primary_10_1007_s11427_020_1745_0
crossref_primary_10_1261_rna_070417_119
crossref_primary_10_1093_nar_gkaa685
crossref_primary_10_1016_j_cobme_2017_10_001
crossref_primary_10_1038_s41586_021_03440_3
crossref_primary_10_1038_s41421_020_0160_4
crossref_primary_10_1038_s41564_018_0353_x
crossref_primary_10_1016_j_chom_2016_01_015
crossref_primary_10_1002_wrna_1804
crossref_primary_10_1093_nar_gky844
crossref_primary_10_1002_INMD_20250052
crossref_primary_10_3390_ijms24086894
crossref_primary_10_1002_anie_202214987
crossref_primary_10_1146_annurev_micro_091014_104441
crossref_primary_10_1038_s41579_022_00793_y
crossref_primary_10_3389_fmicb_2019_02471
crossref_primary_10_1016_j_cub_2020_08_028
crossref_primary_10_1128_JB_00897_15
crossref_primary_10_3389_fgene_2015_00320
crossref_primary_10_1016_j_jmb_2018_08_030
crossref_primary_10_1016_j_cell_2018_09_001
crossref_primary_10_1016_j_molcel_2018_07_013
crossref_primary_10_1016_j_cell_2022_05_003
crossref_primary_10_1016_j_jmb_2018_06_029
crossref_primary_10_3389_fmicb_2022_876174
crossref_primary_10_1016_j_ymeth_2022_06_002
crossref_primary_10_1038_nrmicro3569
crossref_primary_10_1042_BST20220289
crossref_primary_10_1134_S0006297921100114
crossref_primary_10_3390_biom10111523
crossref_primary_10_1038_s41564_019_0612_5
crossref_primary_10_1128_JB_00747_15
crossref_primary_10_1371_journal_pone_0176221
crossref_primary_10_1016_j_cell_2016_12_009
crossref_primary_10_1016_j_molcel_2018_10_048
crossref_primary_10_1016_j_febslet_2015_09_005
crossref_primary_10_1038_s41467_021_25337_5
crossref_primary_10_1126_science_aad5147
crossref_primary_10_1261_rna_079206_122
crossref_primary_10_1089_hum_2015_091
crossref_primary_10_1128_JCM_01307_18
crossref_primary_10_1146_annurev_genet_022120_112523
crossref_primary_10_3390_genes16080896
crossref_primary_10_1016_j_tim_2016_09_012
crossref_primary_10_1038_s41586_023_06902_y
crossref_primary_10_1126_science_aaf2851
crossref_primary_10_1016_j_bcmd_2016_09_003
crossref_primary_10_3390_ijms23158515
crossref_primary_10_1016_j_chom_2017_07_016
crossref_primary_10_1016_j_biotechadv_2016_08_002
crossref_primary_10_1016_j_molcel_2020_12_033
crossref_primary_10_1093_nar_gkv793
crossref_primary_10_1093_nsr_nwy150
crossref_primary_10_1074_jbc_REV120_007034
crossref_primary_10_1016_j_ymeth_2019_07_015
crossref_primary_10_1016_j_trac_2023_117431
crossref_primary_10_1038_s41564_022_01256_z
crossref_primary_10_1038_s41564_022_01316_4
crossref_primary_10_1016_j_jbiotec_2023_11_004
crossref_primary_10_1016_j_meegid_2023_105445
crossref_primary_10_1261_rna_078739_121
crossref_primary_10_3389_fmicb_2021_657404
crossref_primary_10_1093_nar_gkw891
crossref_primary_10_1093_nar_gkab1130
crossref_primary_10_1074_jbc_M115_704189
crossref_primary_10_1016_j_chom_2019_01_009
crossref_primary_10_1016_j_chom_2025_06_005
crossref_primary_10_1002_ange_202214987
crossref_primary_10_1038_s41586_019_1894_8
crossref_primary_10_7554_eLife_45393
crossref_primary_10_1093_nar_gkae603
crossref_primary_10_1016_j_micpath_2025_107943
crossref_primary_10_1126_science_aar6245
crossref_primary_10_1016_j_molcel_2019_06_014
crossref_primary_10_1016_j_molcel_2019_06_013
crossref_primary_10_1038_s41467_019_14222_x
crossref_primary_10_3390_antibiotics8010018
crossref_primary_10_7554_eLife_27601
crossref_primary_10_1016_j_chom_2022_03_001
crossref_primary_10_1093_nar_gkx791
crossref_primary_10_1016_j_molcel_2018_01_025
crossref_primary_10_3389_fmicb_2022_1046388
crossref_primary_10_1038_s41586_021_03206_x
crossref_primary_10_1038_s41422_019_0151_x
crossref_primary_10_1002_cmdc_202300359
crossref_primary_10_1016_j_cell_2015_12_053
crossref_primary_10_1016_j_ijbiomac_2023_124054
crossref_primary_10_1038_s41586_023_06620_5
crossref_primary_10_1093_nar_gkae856
crossref_primary_10_1007_s00018_019_03252_9
crossref_primary_10_1016_j_jbc_2022_101591
crossref_primary_10_1016_j_biotechadv_2019_03_016
crossref_primary_10_1016_j_chom_2024_11_005
crossref_primary_10_1093_nar_gkab1193
crossref_primary_10_1016_j_foodres_2021_110904
crossref_primary_10_1016_j_jmb_2024_168448
crossref_primary_10_1093_nar_gkab1190
crossref_primary_10_1126_science_aaf5573
crossref_primary_10_1186_s11658_024_00581_x
crossref_primary_10_1016_j_molcel_2022_10_028
crossref_primary_10_1371_journal_pone_0170552
crossref_primary_10_1016_j_mib_2017_11_005
crossref_primary_10_1534_g3_116_032482
crossref_primary_10_1016_j_molcel_2016_08_038
crossref_primary_10_1016_j_synbio_2020_09_003
crossref_primary_10_1016_j_biotechadv_2017_11_008
crossref_primary_10_1016_j_cell_2018_10_052
crossref_primary_10_1093_nar_gkab590
crossref_primary_10_1038_s42003_022_03187_1
crossref_primary_10_1186_s12575_020_00135_3
crossref_primary_10_7554_eLife_55852
crossref_primary_10_1186_s12864_016_3040_4
crossref_primary_10_1093_femsre_fuae020
crossref_primary_10_1038_s41580_021_00371_9
crossref_primary_10_3389_fmicb_2023_1254891
crossref_primary_10_1016_j_ab_2020_113762
crossref_primary_10_1038_nature21719
crossref_primary_10_1089_crispr_2020_0032
crossref_primary_10_1016_j_cell_2017_11_032
crossref_primary_10_1016_j_cell_2018_02_033
crossref_primary_10_1038_s44319_025_00399_4
crossref_primary_10_1186_s12864_020_07178_6
crossref_primary_10_1016_j_cell_2015_12_035
crossref_primary_10_1016_j_molcel_2023_04_003
crossref_primary_10_3892_ijmm_2020_4609
crossref_primary_10_1134_S0006297921040064
crossref_primary_10_1126_science_adv9045
crossref_primary_10_1093_nar_gkad501
crossref_primary_10_1016_j_drudis_2023_103793
crossref_primary_10_1038_s41579_021_00663_z
crossref_primary_10_3390_ijms24032857
crossref_primary_10_1038_s41467_019_10780_2
crossref_primary_10_1038_s41467_024_54629_9
crossref_primary_10_1093_nar_gkab1299
crossref_primary_10_1038_nature15386
crossref_primary_10_1089_crispr_2018_0012
crossref_primary_10_1101_gad_272153_115
crossref_primary_10_1038_nri_2017_78
crossref_primary_10_1016_j_ijmm_2016_08_005
crossref_primary_10_3389_fmicb_2018_01982
crossref_primary_10_1038_s41467_017_02557_2
crossref_primary_10_1016_j_gpb_2021_01_008
crossref_primary_10_1126_science_aar2400
crossref_primary_10_1016_j_molcel_2015_10_030
crossref_primary_10_1093_nar_gkw1274
crossref_primary_10_3389_fmicb_2021_787726
crossref_primary_10_1002_biot_201700586
crossref_primary_10_3389_fmicb_2021_774492
crossref_primary_10_1002_1873_3468_70086
crossref_primary_10_1128_mSphere_00813_20
crossref_primary_10_1016_j_molcel_2016_02_031
crossref_primary_10_1038_s41579_018_0071_7
crossref_primary_10_1016_j_tim_2018_09_006
crossref_primary_10_1093_nar_gkw020
crossref_primary_10_1111_febs_13621
crossref_primary_10_3390_app10249001
crossref_primary_10_1016_j_biotechadv_2024_108323
crossref_primary_10_1016_j_str_2022_05_013
crossref_primary_10_1038_s41467_019_12244_z
crossref_primary_10_1074_jbc_RA119_008728
crossref_primary_10_1093_nar_gkaa176
crossref_primary_10_1093_nar_gkaa298
crossref_primary_10_1016_j_cell_2019_09_003
crossref_primary_10_1107_S2053230X15014776
crossref_primary_10_1016_j_mib_2017_08_003
crossref_primary_10_1093_nar_gkab704
crossref_primary_10_3390_diagnostics12102455
crossref_primary_10_1038_s41564_019_0400_2
crossref_primary_10_3389_fpls_2017_01932
crossref_primary_10_1016_j_cell_2015_06_032
crossref_primary_10_1038_s41586_021_03886_5
crossref_primary_10_1016_j_molcel_2018_11_007
crossref_primary_10_1016_j_sbi_2020_06_010
crossref_primary_10_1073_pnas_1709035114
crossref_primary_10_1016_j_molcel_2018_11_008
crossref_primary_10_1038_s41586_020_1936_2
crossref_primary_10_1146_annurev_micro_041222_024843
crossref_primary_10_1038_nature17945
crossref_primary_10_1007_s10123_021_00208_7
crossref_primary_10_1016_j_molcel_2020_03_033
crossref_primary_10_7554_eLife_53078
crossref_primary_10_1186_s13059_015_0816_9
crossref_primary_10_1038_nature23467
crossref_primary_10_1089_crispr_2021_0046
crossref_primary_10_1042_BCJ20220073
crossref_primary_10_1038_s41467_022_30402_8
crossref_primary_10_1016_j_tibs_2023_10_006
crossref_primary_10_1016_j_scib_2018_03_017
crossref_primary_10_1016_j_tibs_2022_02_004
crossref_primary_10_1093_nar_gkz079
crossref_primary_10_1016_j_cbpa_2019_05_009
crossref_primary_10_1093_nar_gkae1277
crossref_primary_10_1371_journal_pone_0261795
crossref_primary_10_1093_nar_gkae1154
crossref_primary_10_7554_eLife_36734
crossref_primary_10_1016_j_trac_2023_116980
crossref_primary_10_1261_rna_039842_113
crossref_primary_10_1038_s41467_024_47506_y
crossref_primary_10_1038_s41570_017_0078
crossref_primary_10_1038_s41586_019_1909_5
crossref_primary_10_3389_fmicb_2021_671522
crossref_primary_10_1016_j_jmb_2019_01_009
crossref_primary_10_1016_j_molcel_2018_09_018
crossref_primary_10_1016_j_sbi_2016_11_013
crossref_primary_10_1159_000516643
crossref_primary_10_1186_s40779_015_0054_1
crossref_primary_10_1128_MMBR_00011_16
crossref_primary_10_1126_science_aad4234
crossref_primary_10_1093_nar_gkae080
crossref_primary_10_1038_s41587_022_01649_9
crossref_primary_10_1038_s41467_020_15334_5
crossref_primary_10_1111_pbi_13383
crossref_primary_10_1098_rstb_2024_0084
ContentType Journal Article
Copyright Copyright © 2015 Elsevier Inc. All rights reserved.
Copyright_xml – notice: Copyright © 2015 Elsevier Inc. All rights reserved.
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1016/j.cell.2015.04.027
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Biology
EISSN 1097-4172
ExternalDocumentID 25959775
Genre Research Support, Non-U.S. Gov't
Journal Article
GrantInformation_xml – fundername: NIAID NIH HHS
  grantid: DP2 AI104556
GroupedDBID ---
--K
-DZ
-ET
-~X
0R~
0WA
1RT
1~5
29B
2FS
2WC
3EH
4.4
457
4G.
53G
5GY
5RE
62-
6J9
7-5
85S
AACTN
AAEDT
AAEDW
AAFWJ
AAHBH
AAKRW
AAKUH
AALRI
AAMRU
AAVLU
AAXUO
AAYJJ
ABCQX
ABJNI
ABMAC
ABOCM
ACGFO
ACGFS
ACNCT
ADBBV
ADEZE
ADVLN
AEFWE
AENEX
AEXQZ
AFTJW
AGHFR
AGHSJ
AGKMS
AHHHB
AIDAL
AITUG
AKAPO
AKRWK
ALMA_UNASSIGNED_HOLDINGS
AMRAJ
ASPBG
AVWKF
AZFZN
BAWUL
CGR
CS3
CUY
CVF
DIK
DU5
E3Z
EBS
ECM
EIF
EJD
F5P
FCP
FDB
FIRID
HH5
IH2
IHE
IXB
J1W
JIG
K-O
KOO
KQ8
L7B
LX5
M3Z
M41
N9A
NPM
O-L
O9-
OK1
P2P
RIG
RNS
ROL
RPZ
SCP
SDG
SDP
SES
SSZ
TAE
TN5
TR2
TWZ
UKR
UPT
WH7
YZZ
ZCA
7X8
AAYWO
ABDGV
ABUFD
ACVFH
ADCNI
ADXHL
AEUPX
AFPUW
AIGII
AKBMS
AKYEP
APXCP
EFKBS
ID FETCH-LOGICAL-c624t-55491c7d9e23b5baf1354171d06bbbdd83249a1ca9ea055d0cabe4372189b7482
IEDL.DBID 7X8
ISICitedReferencesCount 323
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000355152600022&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1097-4172
IngestDate Sun Nov 09 09:53:14 EST 2025
Thu Apr 03 07:10:32 EDT 2025
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 5
Language English
License Copyright © 2015 Elsevier Inc. All rights reserved.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c624t-55491c7d9e23b5baf1354171d06bbbdd83249a1ca9ea055d0cabe4372189b7482
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://dx.doi.org/10.1016/j.cell.2015.04.027
PMID 25959775
PQID 1683076901
PQPubID 23479
ParticipantIDs proquest_miscellaneous_1683076901
pubmed_primary_25959775
PublicationCentury 2000
PublicationDate 2015-05-21
PublicationDateYYYYMMDD 2015-05-21
PublicationDate_xml – month: 05
  year: 2015
  text: 2015-05-21
  day: 21
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Cell
PublicationTitleAlternate Cell
PublicationYear 2015
References 10510229 - Mol Microbiol. 1999 Sep;33(6):1141-51
18419539 - J Infect Dis. 2008 Apr 1;197(7):1016-27
20072129 - Nature. 2010 Jan 28;463(7280):568-71
21646539 - Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10098-103
18065545 - J Bacteriol. 2008 Feb;190(4):1390-400
19945378 - Cell. 2009 Nov 25;139(5):945-56
21756346 - Biol Direct. 2011;6:38
22160698 - Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21218-22
22227115 - Mol Cell. 2012 Feb 10;45(3):303-13
14576436 - Science. 2003 Oct 24;302(5645):650-4
24187086 - J Bacteriol. 2014 Jan;196(2):310-7
15791728 - J Mol Evol. 2005 Feb;60(2):174-82
9702191 - Mol Cell. 1998 Jul;2(1):55-64
25457165 - Mol Cell. 2014 Nov 20;56(4):518-30
20889749 - J Bacteriol. 2010 Dec;192(23):6291-4
19246744 - Microbiology. 2009 Mar;155(Pt 3):733-40
19095942 - Science. 2008 Dec 19;322(5909):1843-5
25174707 - Nature. 2014 Oct 30;514(7524):633-7
21048762 - Nature. 2010 Nov 4;468(7320):67-71
19363495 - Nat Methods. 2009 May;6(5):343-5
21552286 - Nat Rev Microbiol. 2011 Jun;9(6):467-77
23320564 - Mol Microbiol. 2013 Mar;87(5):1088-99
25458845 - Mol Cell. 2014 Nov 20;56(4):506-17
15758212 - Microbiology. 2005 Mar;151(Pt 3):653-63
19141480 - Genes Dev. 2008 Dec 15;22(24):3489-96
17379808 - Science. 2007 Mar 23;315(5819):1709-12
1310990 - J Biol Chem. 1992 Feb 25;267(6):4207-14
24766887 - Mol Cell. 2014 Apr 24;54(2):234-44
26000476 - Cell. 2015 May 21;161(5):964-6
2649886 - Proc Natl Acad Sci U S A. 1989 Apr;86(8):2627-31
18703739 - Science. 2008 Aug 15;321(5891):960-4
20591188 - Biol Direct. 2010;5:43
23935102 - J Biol Chem. 2013 Sep 27;288(39):27888-97
22521689 - Mol Cell. 2012 Jun 8;46(5):595-605
16079334 - Microbiology. 2005 Aug;151(Pt 8):2551-61
22745249 - Science. 2012 Aug 17;337(6096):816-21
24121684 - Nucleic Acids Res. 2014 Jan;42(2):1129-38
25505143 - Nucleic Acids Res. 2015 Jan;43(1):406-17
24909109 - Nat Rev Microbiol. 2014 Jul;12(7):479-92
References_xml – reference: 25505143 - Nucleic Acids Res. 2015 Jan;43(1):406-17
– reference: 9702191 - Mol Cell. 1998 Jul;2(1):55-64
– reference: 19095942 - Science. 2008 Dec 19;322(5909):1843-5
– reference: 24121684 - Nucleic Acids Res. 2014 Jan;42(2):1129-38
– reference: 20889749 - J Bacteriol. 2010 Dec;192(23):6291-4
– reference: 21552286 - Nat Rev Microbiol. 2011 Jun;9(6):467-77
– reference: 10510229 - Mol Microbiol. 1999 Sep;33(6):1141-51
– reference: 16079334 - Microbiology. 2005 Aug;151(Pt 8):2551-61
– reference: 2649886 - Proc Natl Acad Sci U S A. 1989 Apr;86(8):2627-31
– reference: 24187086 - J Bacteriol. 2014 Jan;196(2):310-7
– reference: 25457165 - Mol Cell. 2014 Nov 20;56(4):518-30
– reference: 1310990 - J Biol Chem. 1992 Feb 25;267(6):4207-14
– reference: 21756346 - Biol Direct. 2011;6:38
– reference: 23935102 - J Biol Chem. 2013 Sep 27;288(39):27888-97
– reference: 20072129 - Nature. 2010 Jan 28;463(7280):568-71
– reference: 22745249 - Science. 2012 Aug 17;337(6096):816-21
– reference: 19246744 - Microbiology. 2009 Mar;155(Pt 3):733-40
– reference: 18703739 - Science. 2008 Aug 15;321(5891):960-4
– reference: 18065545 - J Bacteriol. 2008 Feb;190(4):1390-400
– reference: 18419539 - J Infect Dis. 2008 Apr 1;197(7):1016-27
– reference: 23320564 - Mol Microbiol. 2013 Mar;87(5):1088-99
– reference: 15758212 - Microbiology. 2005 Mar;151(Pt 3):653-63
– reference: 20591188 - Biol Direct. 2010;5:43
– reference: 26000476 - Cell. 2015 May 21;161(5):964-6
– reference: 14576436 - Science. 2003 Oct 24;302(5645):650-4
– reference: 17379808 - Science. 2007 Mar 23;315(5819):1709-12
– reference: 25458845 - Mol Cell. 2014 Nov 20;56(4):506-17
– reference: 22227115 - Mol Cell. 2012 Feb 10;45(3):303-13
– reference: 22521689 - Mol Cell. 2012 Jun 8;46(5):595-605
– reference: 24766887 - Mol Cell. 2014 Apr 24;54(2):234-44
– reference: 19945378 - Cell. 2009 Nov 25;139(5):945-56
– reference: 21646539 - Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10098-103
– reference: 21048762 - Nature. 2010 Nov 4;468(7320):67-71
– reference: 15791728 - J Mol Evol. 2005 Feb;60(2):174-82
– reference: 19363495 - Nat Methods. 2009 May;6(5):343-5
– reference: 25174707 - Nature. 2014 Oct 30;514(7524):633-7
– reference: 19141480 - Genes Dev. 2008 Dec 15;22(24):3489-96
– reference: 22160698 - Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21218-22
– reference: 24909109 - Nat Rev Microbiol. 2014 Jul;12(7):479-92
SSID ssj0008555
Score 2.6174667
Snippet Immune systems must recognize and destroy different pathogens that threaten the host. CRISPR-Cas immune systems protect prokaryotes from viral and plasmid...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 1164
SubjectTerms Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Cas Systems
DNA - genetics
DNA - metabolism
Ribonucleoproteins - metabolism
RNA - genetics
RNA - metabolism
Staphylococcus epidermidis - immunology
Staphylococcus epidermidis - metabolism
Staphylococcus epidermidis - virology
Transcription, Genetic
Title Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity
URI https://www.ncbi.nlm.nih.gov/pubmed/25959775
https://www.proquest.com/docview/1683076901
Volume 161
WOSCitedRecordID wos000355152600022&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1JS8QwFA7qKHhxX8aNCF6DXZKmOclQHSxiGUaFuQ3ZCoK0o60D8-_NazvMSRC8NJcGwsvL29_7ELoRMaTnwpzoiCtC84gR5ZuASOEprrQEk74Bm-BZFk8mYtQF3KqurHIpExtBbUoNMfJbP4odOwJ80t3skwBqFGRXOwiNddQLnSkDXM0nq2nhMWtQTyHJSqjT1F3TTFvfBYFxKO1izajTgP9uYjaqZrj730PuoZ3OyMSDliv20ZotDtBWCzu5OERPSUlq0FFLieH-vc8GWBYGj92afFg5d3IGtz2MGHxVnKYpTsbpy2hMElnhtOkrqRdH6G348Jo8kg5Uwd1GQGvizAfha26EDULFlMz9kDna-MaLlFLGuBdOhfS1FFZ6jBlPS2UhuefHQnEaB8dooygLe4qwiaVyDlioeK4ptc5zk8wzgaE2lFbIsI-ul1SaOqYFgsvClt_VdEWnPjppST2dtdM1ps4fg5l47OwPu8_RNtwgZPMD_wL1cvdk7SXa1PP6vfq6arjBfbPR8w8IVrx0
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Co-transcriptional+DNA+and+RNA+Cleavage+during+Type+III+CRISPR-Cas+Immunity&rft.jtitle=Cell&rft.au=Samai%2C+Poulami&rft.au=Pyenson%2C+Nora&rft.au=Jiang%2C+Wenyan&rft.au=Goldberg%2C+Gregory+W&rft.date=2015-05-21&rft.eissn=1097-4172&rft.volume=161&rft.issue=5&rft.spage=1164&rft_id=info:doi/10.1016%2Fj.cell.2015.04.027&rft_id=info%3Apmid%2F25959775&rft_id=info%3Apmid%2F25959775&rft.externalDocID=25959775
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1097-4172&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1097-4172&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1097-4172&client=summon