The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses

Vector arthropods control arbovirus replication and spread through antiviral innate immune responses including RNA interference (RNAi) pathways. Arbovirus infections have been shown to induce the exogenous small interfering RNA (siRNA) and Piwi-interacting RNA (piRNA) pathways, but direct antiviral...

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Vydáno v:	PLoS neglected tropical diseases Ročník 11; číslo 1; s. e0005272
Hlavní autoři:	Dietrich, Isabelle, Shi, Xiaohong, McFarlane, Melanie, Watson, Mick, Blomström, Anne-Lie, Skelton, Jessica K., Kohl, Alain, Elliott, Richard M., Schnettler, Esther
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 06.01.2017 Public Library of Science (PLoS)
Témata:	Aedes - genetics Aedes - immunology Aedes - virology Analysis Animals Aquatic insects Biology and life sciences Bunyaviruses Ceratopogonidae - genetics Ceratopogonidae - immunology Ceratopogonidae - virology Colleges & universities Councils Genomes Infections Medicine and Health Sciences Mosquitoes Orthobunyavirus - physiology Proteins Public health Research and analysis methods RNA Interference RNA polymerase RNA, Small Interfering - genetics RNA, Small Interfering - immunology Tropical diseases Tropism Virology Virus-vector relationships Viruses Glasgow Scotland Scotland Edinburgh Scotland United Kingdom > UK
ISSN:	1935-2735, 1935-2727, 1935-2735
On-line přístup:	Získat plný text
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Shrnutí:	Vector arthropods control arbovirus replication and spread through antiviral innate immune responses including RNA interference (RNAi) pathways. Arbovirus infections have been shown to induce the exogenous small interfering RNA (siRNA) and Piwi-interacting RNA (piRNA) pathways, but direct antiviral activity by these host responses in mosquito cells has only been demonstrated against a limited number of positive-strand RNA arboviruses. For bunyaviruses in general, the relative contribution of small RNA pathways in antiviral defences is unknown. The genus Orthobunyavirus in the Bunyaviridae family harbours a diverse range of mosquito-, midge- and tick-borne arboviruses. We hypothesized that differences in the antiviral RNAi response in vector versus non-vector cells may exist and that could influence viral host range. Using Aedes aegypti-derived mosquito cells, mosquito-borne orthobunyaviruses and midge-borne orthobunyaviruses we showed that bunyavirus infection commonly induced the production of small RNAs and the effects of the small RNA pathways on individual viruses differ in specific vector-arbovirus interactions. These findings have important implications for our understanding of antiviral RNAi pathways and orthobunyavirus-vector interactions and tropism.
Bibliografie:	new_version ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Current address: Nuffield Department of Experimental Medicine, University of Oxford, Oxford, United Kingdom Current address: BNITM, Hamburg, Germany Current address: Section of Virology, Department of Medicine, Imperial College London, St Mary's Hospital, London, United Kingdom Conceptualization: ID ES RME XS ALB AK.Data curation: MW ES AK.Formal analysis: ID MM MW ALB XS ES.Investigation: ID XS MM ALB JKS MW.Methodology: ID ES RME AK XS ALB MW.Project administration: ES AK.Resources: MW.Software: MW.Supervision: ES RME AK.Validation: ID XS JKS ALB MM.Visualization: ID XS ALB MM MW ES.Writing – original draft: ID AK ES.Writing – review & editing: ID XS MM MW ALB AK RME ES. Current address: Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden The authors have declared that no competing interests exist.
ISSN:	1935-2735 1935-2727 1935-2735
DOI:	10.1371/journal.pntd.0005272