rice acyl-CoA-binding protein gene family: phylogeny, expression and functional analysis

• Acyl-CoA-binding proteins (ACBPs) show conservation in an acyl-CoA-binding domain (ACB domain) which binds acyl-CoA esters. Previous studies on plant ACBPs focused on eudicots, Arabidopsis and Brassica. Here, we report on the phylogeny and characterization of the ACBP family from the monocot Oryza...

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Vydáno v:	The New phytologist Ročník 189; číslo 4; s. 1170 - 1184
Hlavní autoři:	Meng, Wei, Su, Yvonne C.F, Saunders, Richard M.K, Chye, Mee-Len
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford, UK Blackwell Publishing Ltd 01.03.2011 John Wiley & Sons Wiley Subscription Services, Inc
Témata:	acyl‐CoA esters acyl‐CoA‐binding protein (ACBP) Amino acids Ankyrins Arabidopsis biotic stress Brassica chemistry Diazepam Binding Inhibitor Diazepam Binding Inhibitor - chemistry Diazepam Binding Inhibitor - metabolism Domains embryophytes Esters Esters - metabolism Evolution expression profiles Functional analysis Gene Dosage Gene Dosage - genetics Gene expression Gene Expression Profiling Gene Expression Regulation, Plant genes genetics Genomes Histidine Histidine - metabolism isolation & purification Kinetics messenger RNA metabolism monocot Multigene Family Multigene Family - genetics Nucleotide sequence Oligopeptides Oligopeptides - metabolism Oryza Oryza - genetics Oryza - metabolism Oryza sativa PCR Phylogenetics Phylogeny Plant Proteins Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plants Polymerase chain reaction Protein Structure, Tertiary Proteins Recombinant Proteins Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Recombinants Reverse Transcriptase Polymerase Chain Reaction Rice rice protein stress response Substrates Transcription
ISSN:	0028-646X, 1469-8137, 1469-8137
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Shrnutí:	• Acyl-CoA-binding proteins (ACBPs) show conservation in an acyl-CoA-binding domain (ACB domain) which binds acyl-CoA esters. Previous studies on plant ACBPs focused on eudicots, Arabidopsis and Brassica. Here, we report on the phylogeny and characterization of the ACBP family from the monocot Oryza sativa (rice). • Phylogenetic analyses were conducted using 16 plant genomes. Expression profiles of rice ACBPs under normal growth, as well as biotic and abiotic stress conditions, were examined by quantitative real-time reverse-transcription polymerase chain reactions. In vitro acyl-CoA-binding assays were conducted using recombinant (His)₆-tagged ACBPs. • The ACBP family diversified as land plants evolved. Classes I and IV show lineage-specific gene expansion. Classes II and III are closely related phylogenetically. As in the eudicot Arabidopsis, six genes (designated OsACBP1 to OsACBP6) encode rice ACBPs, but their distribution into various classes differed from Arabidopsis. Rice ACBP mRNAs showed ubiquitous expression and OsACBP4, OsACBP5 and OsACBP6 were stress-responsive. All recombinant rice ACBPs bind [¹⁴C]linolenoyl-CoA besides having specific substrates. • Phylogeny, gene expression and biochemical analyses suggest that paralogues within and across classes are not redundant proteins. In addition to performing conserved basal functions, multidomain rice ACBPs appear to be associated with stress responses.
Bibliografie:	http://dx.doi.org/10.1111/j.1469-8137.2010.03546.x ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0028-646X 1469-8137 1469-8137
DOI:	10.1111/j.1469-8137.2010.03546.x