Practical approaches to plant volatile analysis

Plants emit volatile organic compounds (VOCs) that play important roles in their interaction with the environment and have a major impact on atmospheric chemistry. The development of static and dynamic techniques for headspace collection of volatiles in combination with gas chromatography-mass spect...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology Vol. 45; no. 4; pp. 540 - 560
Main Authors: Tholl, Dorothea, Boland, Wilhelm, Hansel, Armin, Loreto, Francesco, Röse, Ursula S.R, Schnitzler, Jörg-Peter
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Science Ltd 01.02.2006
Blackwell Science Ltd
Blackwell Science
Subjects:
Agronomy. Soil science and plant productions
atmospheric chemistry
Biological and medical sciences
biosynthesis
chemistry
emissions
enzymes
Fundamental and applied biological sciences. Psychology
gas chromatography
Gas Chromatography-Mass Spectrometry
GC‐MS
Genetic engineering applications
Genetics and breeding of economic plants
headspace sampling
in vivo studies
mass spectrometry
monitoring
Plant breeding: fundamental aspects and methodology
plant ecology
Plants
Plants - chemistry
real time analysis
SPME
VOC
volatile organic compounds
Volatilization
GC-MS
real time analysis
headspace sampling
SPME
Development
Collection
Biosynthesis
Volatile organic compound
Review
VOC
ISSN:0960-7412, 1365-313X
Online Access:Get full text
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Summary:Plants emit volatile organic compounds (VOCs) that play important roles in their interaction with the environment and have a major impact on atmospheric chemistry. The development of static and dynamic techniques for headspace collection of volatiles in combination with gas chromatography-mass spectrometry analysis has significantly improved our understanding of the biosynthesis and ecology of plant VOCs. Advances in automated analysis of VOCs have allowed the monitoring of fast changes in VOC emissions and facilitated in vivo studies of VOC biosynthesis. This review presents an overview of methods for the analysis of plant VOCs, including their advantages and disadvantages, with a focus on the latest technical developments. It provides guidance on how to select appropriate instrumentation and protocols for biochemical, physiological and ecologically relevant applications. These include headspace analyses of plant VOCs emitted by the whole organism, organs or enzymes as well as advanced on-line analysis methods for simultaneous measurements of VOC emissions with other physiological parameters.
Bibliography:http://dx.doi.org/10.1111/j.1365-313X.2005.02612.x
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ISSN:0960-7412
1365-313X
DOI:10.1111/j.1365-313x.2005.02612.x