Midgut Microbiota of the Malaria Mosquito Vector Anopheles gambiae and Interactions with Plasmodium falciparum Infection

The susceptibility of Anopheles mosquitoes to Plasmodium infections relies on complex interactions between the insect vector and the malaria parasite. A number of studies have shown that the mosquito innate immune responses play an important role in controlling the malaria infection and that the str...

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Veröffentlicht in:PLoS pathogens Jg. 8; H. 5; S. e1002742
Hauptverfasser: Boissière, Anne, Tchioffo, Majoline T., Bachar, Dipankar, Abate, Luc, Marie, Alexandra, Nsango, Sandrine E., Shahbazkia, Hamid R., Awono-Ambene, Parfait H., Levashina, Elena A., Christen, Richard, Morlais, Isabelle
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 01.05.2012
Public Library of Science (PLoS)
Schlagworte:
Animals
Anopheles - genetics
Anopheles - immunology
Anopheles - microbiology
Anopheles - parasitology
Aquatic insects
Biodiversity
Biology
Breeding sites
Digestive System - microbiology
Digestive System - parasitology
Disease transmission
Enterobacter - genetics
Enterobacter - immunology
Environmental factors
Flora
Gene Expression Regulation
Genomics
Host-Parasite Interactions
Insect Vectors - genetics
Insect Vectors - immunology
Insect Vectors - microbiology
Larvae
Life Sciences
Malaria
Malaria, Falciparum - genetics
Malaria, Falciparum - immunology
Malaria, Falciparum - parasitology
Microbiology
Mosquitoes
Parasites
Plasmodium falciparum - genetics
Plasmodium falciparum - immunology
Plasmodium falciparum - microbiology
Populations and Evolution
Vector-borne diseases
ISSN:1553-7374, 1553-7366, 1553-7374
Online-Zugang:Volltext
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Zusammenfassung:The susceptibility of Anopheles mosquitoes to Plasmodium infections relies on complex interactions between the insect vector and the malaria parasite. A number of studies have shown that the mosquito innate immune responses play an important role in controlling the malaria infection and that the strength of parasite clearance is under genetic control, but little is known about the influence of environmental factors on the transmission success. We present here evidence that the composition of the vector gut microbiota is one of the major components that determine the outcome of mosquito infections. A. gambiae mosquitoes collected in natural breeding sites from Cameroon were experimentally challenged with a wild P. falciparum isolate, and their gut bacterial content was submitted for pyrosequencing analysis. The meta-taxogenomic approach revealed a broader richness of the midgut bacterial flora than previously described. Unexpectedly, the majority of bacterial species were found in only a small proportion of mosquitoes, and only 20 genera were shared by 80% of individuals. We show that observed differences in gut bacterial flora of adult mosquitoes is a result of breeding in distinct sites, suggesting that the native aquatic source where larvae were grown determines the composition of the midgut microbiota. Importantly, the abundance of Enterobacteriaceae in the mosquito midgut correlates significantly with the Plasmodium infection status. This striking relationship highlights the role of natural gut environment in parasite transmission. Deciphering microbe-pathogen interactions offers new perspectives to control disease transmission.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Conceived and designed the experiments: AB RC IM. Performed the experiments: AB MTT LA AM SEN PHAA RC IM. Analyzed the data: AB DB LA HRS RC IM. Contributed reagents/materials/analysis tools: EAL RC IM. Wrote the paper: AB RC IM.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1002742