Metagenomic analysis of coastal RNA virus communities

RNA viruses infect marine organisms from bacteria to whales, but RNA virus communities in the sea remain essentially unknown. Reverse-transcribed whole-genome shotgun sequencing was used to characterize the diversity of uncultivated marine RNA virus assemblages. A diverse assemblage of RNA viruses,...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) Vol. 312; no. 5781; p. 1795
Main Authors: Culley, Alexander I, Lang, Andrew S, Suttle, Curtis A
Format: Journal Article
Language:English
Published: United States 23.06.2006
Subjects:
Animals
Arthropods - virology
Contig Mapping
DNA, Complementary
Ecosystem
Genetic Variation
Genome, Viral
Genomic Library
Genomics
Genotype
Molecular Sequence Data
Pacific Ocean
Phylogeny
Picornaviridae - classification
Picornaviridae - genetics
Plants - virology
Reverse Transcriptase Polymerase Chain Reaction
RNA Viruses - genetics
RNA, Viral - classification
RNA, Viral - genetics
RNA, Viral - isolation & purification
Seawater - virology
Sequence Analysis, DNA
Synteny
Tombusviridae - classification
Tombusviridae - genetics
Pacific Ocean
ISSN:1095-9203, 1095-9203
Online Access:Get more information
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Summary:RNA viruses infect marine organisms from bacteria to whales, but RNA virus communities in the sea remain essentially unknown. Reverse-transcribed whole-genome shotgun sequencing was used to characterize the diversity of uncultivated marine RNA virus assemblages. A diverse assemblage of RNA viruses, including a broad group of marine picorna-like viruses, and distant relatives of viruses infecting arthropods and higher plants were found. Communities were dominated by distinct genotypes with small genome sizes, and we completely assembled the genomes of several hitherto undiscovered viruses. Our results show that the oceans are a reservoir of previously unknown RNA viruses.
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.1127404