Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

Background Replacement of radioligand binding assays with antibody-antigen interaction-based approaches for quantitative analysis of G protein-coupled receptor (GPCR) levels requires the use of purified protein standards containing the antigen. GPCRs in general and cannabinoid CB 1 receptor in parti...

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Veröffentlicht in:	Microbial cell factories Jg. 21; H. 1; S. 1 - 18
Hauptverfasser:	Saumell-Esnaola, Miquel, Elejaga-Jimeno, Ainhoa, Echeazarra, Leyre, Borrega-Román, Leire, Barrondo, Sergio, López de Jesús, Maider, González-Burguera, Imanol, Gómez-Caballero, Alberto, Goicolea, María Aranzazu, Sallés, Joan, García del Caño, Gontzal
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London BioMed Central 15.09.2022 BioMed Central Ltd Springer Nature B.V BMC
Schlagworte:	Antibodies Antigens Applied Microbiology Aqueous solutions Binding Binding sites Biological properties Biological samples Biotechnology Cannabinoid CB1 receptor antibodies Cannabinoid CB1 receptors Cannabinoids Carboxy-terminal tail Cell membranes Cell receptors Chemical properties Chemistry Chemistry and Materials Science Cloning Cost analysis Density Design Enzymology G protein-coupled receptors Genetic Engineering GPCR expression analysis Homology Hydrophobicity Ligands Medical research Membrane proteins Membranes Methods Microbial Genetics and Genomics Microbiology Peptides Pharmaceutical industry Physiological aspects Plasmids Production processes Protein engineering Protein expression Proteins Quantitative analysis Quantitative Western blot Radioactivity Radioligand saturation binding Receptor density Receptors Recombinant proteins Residues Saturation Soluble recombinant protein standards Synaptosomes Transmembrane domains Western immunoblotting Spain Cannabinoid CB Soluble recombinant protein standards GST fusion proteins GPCR expression analysis receptor antibodies Carboxy-terminal tail Radioligand saturation binding Quantitative Western blot
ISSN:	1475-2859, 1475-2859
Online-Zugang:	Volltext
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