Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

Abstract Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has...

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Vydáno v:	FEMS microbiology reviews Ročník 36; číslo 2; s. 380 - 407
Hlavní autoři:	Croxatto, Antony, Prod'hom, Guy, Greub, Gilbert
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford, UK Blackwell Publishing Ltd 01.03.2012 Blackwell Oxford University Press
Témata:	Animals Bacteria - chemistry Bacteria - isolation & purification Bacterial Infections - diagnosis Bacterial Infections - microbiology bacteriology Biological and medical sciences Clinical microbiology diagnostic microbiology Diagnostic systems Diagnostic Techniques and Procedures Epidemiology Fundamental and applied biological sciences. Psychology Fungi Fungi - chemistry Fungi - isolation & purification Gene sequencing Gram-negative bacteria Humans Identification Ionization Ions Laboratories MALDI‐TOF Mass spectrometry Mass spectroscopy microbial identification Microbiology Microorganisms Mycoses - diagnosis Mycoses - microbiology Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - economics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectroscopy Strains (organisms) Yeast mass spectrometry bacteriology diagnostic microbiology microbial identification MALDI-TOF Identification Review Matrix assisted laser desorption ionization
ISSN:	0168-6445, 1574-6976, 1574-6976
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Popis
Shrnutí:	Abstract Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification. Use of MALDI-TOF mass spectrometry in routine microbial identification increased much during last years and it is time to summarize and review all available data, to help clinical microbiologist to use this quantum leap technology.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Undefined-1 ObjectType-Feature-3 ObjectType-Review-4 content type line 23
ISSN:	0168-6445 1574-6976 1574-6976
DOI:	10.1111/j.1574-6976.2011.00298.x