Refractive index to evaluate staining specificity of extracellular vesicles by flow cytometry

Extracellular vesicles (EVs) in plasma are commonly identified by staining with antibodies and generic dyes, but the specificity of antibodies and dyes to stain EVs is often unknown. Previously, we showed that platelet-depleted platelet concentrate contains two populations of particles >200 nm, o...

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Bibliographic Details
Published in:	Journal of extracellular vesicles Vol. 8; no. 1; pp. 1643671 - n/a
Main Authors:	de Rond, L., Libregts, S.F.W.M., Rikkert, L.G., Hau, C.M., van der Pol, E., Nieuwland, R., van Leeuwen, T.G., Coumans, F.A.W.
Format:	Journal Article
Language:	English
Published:	Sweden Taylor & Francis 01.12.2019 John Wiley & Sons, Inc Wiley
Subjects:	Antibodies Body fluids Calcein Centrifugation Centrifuges Detergents Dyes Exosomes Extracellular vesicles extracellular vesicles (EVs) Flow cytometry light scattering Lipids Lipoproteins microparticle Plasma Platelets Population studies Proteins specificity Stains Stains & staining Transmission electron microscopy Netherlands specificity flow cytometry light scattering extracellular vesicles (EVs) Exosomes microparticle
ISSN:	2001-3078, 2001-3078
Online Access:	Get full text
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