CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes

CRISPR knockout screens outperform shRNA and CRISPR-interference screens in a side-by-side comparison. High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced shor...

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Veröffentlicht in:	Nature biotechnology Jg. 34; H. 6; S. 631 - 633
Hauptverfasser:	Evers, Bastiaan, Jastrzebski, Katarzyna, Heijmans, Jeroen P M, Grernrum, Wipawadee, Beijersbergen, Roderick L, Bernards, Rene
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Nature Publishing Group US 01.06.2016 Nature Publishing Group
Schlagworte:	49/47 631/1647/1511 631/1647/2163 631/208/191 Agriculture Bioinformatics Biomedical Engineering/Biotechnology Biomedicine Biotechnology brief-communication Clustered Regularly Interspaced Short Palindromic Repeats - genetics Comparative analysis Gene Expression Profiling - methods Gene Knockout Techniques - methods Genetic Markers - genetics Genetic screening Genetic testing Genetic Testing - methods High-throughput screening (Biochemical assaying) Life Sciences Oncology R&D Reagents Research & development Ribonucleic acid RNA RNA, Small Interfering - genetics
ISSN:	1087-0156, 1546-1696, 1546-1696
Online-Zugang:	Volltext
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