Production and characterization of mouse monoclonal antibodies reactive to Chikungunya envelope E2 glycoprotein

Chikungunya fever is an arbovirosis of major impact in public health in Asia and Africa. Chikungunya (CHIK) virus is member of the genus Alphavirus and belongs to the Semliki Forest (SF) antigenic complex. We describe for the first time a panel of monoclonal antibodies (MAbs) reactive to CHIK envelo...

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Bibliographic Details
Published in:	Virology (New York, N.Y.) Vol. 371; no. 1; pp. 185 - 195
Main Authors:	Bréhin, Anne-Claire, Rubrecht, Laetitia, Navarro-Sanchez, Martha Erika, Maréchal, Valérie, Frenkiel, Marie-Pascale, Lapalud, Priscilla, Laune, Daniel, Sall, Amadou Alpha, Desprès, Philippe
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 05.02.2008
Subjects:	Alphavirus Alphaviruses Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigen-Antibody Reactions Antigen-capture ELISA Antigens, Viral - genetics Cercopithecus aethiops Chikungunya virus Chikungunya virus - immunology cross reaction Drosophila Drosophila - cytology Drosophila - metabolism Envelope E2 glycoprotein Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescent Antibody Technique, Indirect glycoproteins Glycoproteins - genetics Glycoproteins - immunology Immunoblot analysis Immunofluorescence assay Indirect ELISA Infectious Disease Mice Mice, Inbred BALB C Monoclonal antibodies Recombinant E2 antigen Recombinant Proteins - immunology Solubility Vero Cells viral antigens Viral diagnosis viral envelope proteins Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology viral proteins Viral diagnosis Immunofluorescence assay Antigen-capture ELISA Chikungunya virus Envelope E2 glycoprotein Monoclonal antibodies Recombinant E2 antigen Immunoblot analysis Alphaviruses Indirect ELISA
ISSN:	0042-6822, 1096-0341
Online Access:	Get full text
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