Production and characterization of mouse monoclonal antibodies reactive to Chikungunya envelope E2 glycoprotein

Chikungunya fever is an arbovirosis of major impact in public health in Asia and Africa. Chikungunya (CHIK) virus is member of the genus Alphavirus and belongs to the Semliki Forest (SF) antigenic complex. We describe for the first time a panel of monoclonal antibodies (MAbs) reactive to CHIK envelo...

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Vydané v:Virology (New York, N.Y.) Ročník 371; číslo 1; s. 185 - 195
Hlavní autori: Bréhin, Anne-Claire, Rubrecht, Laetitia, Navarro-Sanchez, Martha Erika, Maréchal, Valérie, Frenkiel, Marie-Pascale, Lapalud, Priscilla, Laune, Daniel, Sall, Amadou Alpha, Desprès, Philippe
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Elsevier Inc 05.02.2008
Predmet:
Alphavirus
Alphaviruses
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - immunology
Antigen-Antibody Reactions
Antigen-capture ELISA
Antigens, Viral - genetics
Cercopithecus aethiops
Chikungunya virus
Chikungunya virus - immunology
cross reaction
Drosophila
Drosophila - cytology
Drosophila - metabolism
Envelope E2 glycoprotein
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Antibody Technique, Indirect
glycoproteins
Glycoproteins - genetics
Glycoproteins - immunology
Immunoblot analysis
Immunofluorescence assay
Indirect ELISA
Infectious Disease
Mice
Mice, Inbred BALB C
Monoclonal antibodies
Recombinant E2 antigen
Recombinant Proteins - immunology
Solubility
Vero Cells
viral antigens
Viral diagnosis
viral envelope proteins
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
viral proteins
Viral diagnosis
Immunofluorescence assay
Antigen-capture ELISA
Chikungunya virus
Envelope E2 glycoprotein
Monoclonal antibodies
Recombinant E2 antigen
Immunoblot analysis
Alphaviruses
Indirect ELISA
ISSN:0042-6822, 1096-0341
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Shrnutí:Chikungunya fever is an arbovirosis of major impact in public health in Asia and Africa. Chikungunya (CHIK) virus is member of the genus Alphavirus and belongs to the Semliki Forest (SF) antigenic complex. We describe for the first time a panel of monoclonal antibodies (MAbs) reactive to CHIK envelope E2 glycoprotein. For the screening of E2-specific MAbs, we expressed a recombinant soluble CHIK E2 protein in Drosophila S2 cells. Analyzed by immunological methods, MAbs 3C3, 3E4, and 8A4 were selected on the basis of their reactivity. Their epitopes are located to the outer surface of CHIK virion. These MAbs have no cross reactivity with related members of SF antigenic complex with the notable exception of Igbo-Ora virus. Anti-CHIK E2 MAbs 3C3, 3E4, and 8A4 should be helpful for studying the biology of CHIK virus and pathogenesis of disease. The combination of 8A4 and 3E4 is suitable for developing a specific antigen-capture ELISA.
Bibliografia:http://dx.doi.org/10.1016/j.virol.2007.09.028
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2007.09.028