Relative expression of hormone receptors by endothelial and smooth muscle cells in proliferative and non-proliferative areas of congenital arteriovenous malformations

Background Episodic growth due to microvascular proliferations (MVP) has been reported in congenital arteriovenous malformations (AVM), which are normally quiescent lesions composed of mature malformed vessels. Since AVM also may worsen under conditions of hormonal dysregulation, we hypothesized tha...

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Vydané v:European journal of medical research Ročník 28; číslo 1; s. 1 - 12
Hlavní autori: Utami, A. M., Halfwerk, J. B. G., de Boer, O. J., Mackaaij, C., Pabittei, D. R., van der Horst, C. M. A. M., Meijer-Jorna, L. B., van der Wal, A. C.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London BioMed Central 20.10.2023
BioMed Central Ltd
Springer Nature B.V
BMC
Predmet:
Analysis
Angiogenesis
Antibodies
Arteriovenous malformations
Biomedicine
Child development
Digitization
Endothelium
Estrogen
Estrogens
Follicle-stimulating hormone
Follicles
Genetic disorders
Growth hormones
Hormone receptor
Immunohistochemistry
Infectious Diseases
Internal Medicine
Localization
Medical research
Medicine
Medicine & Public Health
Medicine, Experimental
Microvascular proliferation
Oncology
Polymerase chain reaction
Progesterone
Skin
Smooth muscle
Somatotropin
Statistical analysis
Surgery
Vascular malformations
United States > US
Netherlands
Vascular malformations
Arteriovenous malformations
Hormone receptor
Microvascular proliferation
ISSN:2047-783X, 0949-2321, 2047-783X
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Shrnutí:Background Episodic growth due to microvascular proliferations (MVP) has been reported in congenital arteriovenous malformations (AVM), which are normally quiescent lesions composed of mature malformed vessels. Since AVM also may worsen under conditions of hormonal dysregulation, we hypothesized that hormonal influences may stimulate this process of vasoproliferative growth through potential interactions with hormone receptors (HR). Methods 13 Cases of AVM tissue with histologically documented vasoproliferative growth were analyzed quantitatively for the presence and tissue localization of estrogen receptor (ER), progesterone receptor (PGR), growth hormone receptor (GHR) and follicle-stimulating hormone receptor (FSHR) in relation to resident cells of interest (endothelial cells (EC), smooth muscle cells (SMC) and mast cells (MC)) by applying multiplex immunohistochemistry (IHC) staining. Expression patterns in lesions with MVP and mature vessels were quantified and compared. Available fresh frozen tissues of 3 AVM samples were used to confirm the presence of HR using Reverse-Transcriptase quantitative Polymerase Chain Reaction (RT-qPCR). Results All four HR studied were expressed in all cases within EC and SMC in areas of MVP and mature vessels, but not in normal skin tissue. ER, GHR, and FSHR showed more expression in EC of MVP and in SMC of mature vessels. RT-qPCR confirmed presence of all 4 HR in both areas. Conclusion Expression of ER, PGR, GHR, and FSHR in vasoproliferative areas of congenital AVM could explain onset of sudden symptomatic growth, as has observed in a subpopulation of patients. These findings may have implications for eventual anti-hormonal targeted therapy in the lesions involved.
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SourceType-Scholarly Journals-1
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ISSN:2047-783X
0949-2321
2047-783X
DOI:10.1186/s40001-023-01436-5