Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19

Diagnostic services for tuberculosis (TB) are not sufficiently accessible in low-resource settings, where most cases occur, which was aggravated by the COVID-19 pandemic. Early diagnosis of pulmonary TB can reduce transmission. Current TB-diagnostics rely on detection of Mycobacterium tuberculosis (...

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Veröffentlicht in:iScience Jg. 26; H. 1; S. 105873
Hauptverfasser: Pierneef, Louise, van Hooij, Anouk, de Jong, Danielle, Tjon Kon Fat, Elisa M., van Meijgaarden, Krista E., Petruccioli, Elisa, Vanini, Valentina, Roukens, Anna H.E., Goletti, Delia, Corstjens, Paul L.A.M., Joosten, Simone A., Geluk, Annemieke, Arbous, M.S., van den Berg, B.M., Cannegieter, S., Cobbaert, C.M., van der Does, A., van Dongen, J.J.M., Eikenboom, J., Feltkamp, M.C.M., Geluk, A., Goeman, J.J., Giera, M., Hankemeier, T., Heemskerk, M.H.M., Hiemstra, P.S., Hokke, C.H., Janse, J.J., Jochems, S.P., Joosten, S.A., Kikkert, M., Lamont, L., Manniën, J., Ottenhoff, T.H.M., del Prado, M.R., Queralt Rosinach, N., Roestenberg, M., Roos, M., Roukens, A.H.E., Smits, H.H., Snijder, E.J., Staal, F.J.T., Trouw, L.A., Tsonaka, R., Verhoeven, A., Visser, L.G., de Vries, J.J.C., van Westerloo, D.J., Wigbers, J., van der Wijk, H.J., van Wissen, R.C., Wuhrer, M., Yazdanbakhsh, M., Zlei, M.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier Inc 20.01.2023
Elsevier BV
The Authors
Elsevier
Schlagworte:
Bacteriology
Q
Science
Virology
Bacteriology
Virology
ISSN:2589-0042, 2589-0042
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Zusammenfassung:Diagnostic services for tuberculosis (TB) are not sufficiently accessible in low-resource settings, where most cases occur, which was aggravated by the COVID-19 pandemic. Early diagnosis of pulmonary TB can reduce transmission. Current TB-diagnostics rely on detection of Mycobacterium tuberculosis (Mtb) in sputum requiring costly, time-consuming methods, and trained staff. In this study, quantitative lateral flow (LF) assays were used to measure levels of seven host proteins in sera from pre-COVID-19 TB patients diagnosed in Europe and latently Mtb-infected individuals (LTBI), and from COVID-19 patients and healthy controls. Analysis of host proteins showed significantly lower levels in LTBI versus TB (AUC:0 · 94) and discriminated healthy individuals from COVID-19 patients (0 · 99) and severe COVID-19 from TB. Importantly, these host proteins allowed treatment monitoring of both respiratory diseases. This study demonstrates the potential of non-sputum LF assays as adjunct diagnostics and treatment monitoring for COVID-19 and TB based on quantitative detection of multiple host biomarkers. [Display omitted] •Quantitative LFAs were used to assess host biomarkers for TB and COVID-19 diagnosis•Combined biomarker levels discriminated TB from latent TB and COVID-19•Host biomarker LFAs can be deployed as adjunct diagnostics within clinical context•Quantitative LFAs enable treatment response monitoring for TB and COVID-19 Virology; Bacteriology
Bibliographie:ObjectType-Article-1
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content type line 23
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2022.105873