Structure and Engineering of Francisella novicida Cas9

The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing. Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA target sequence, thus constricting the range of targetab...

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Vydané v:	Cell Ročník 164; číslo 5; s. 950
Hlavní autori:	Hirano, Hisato, Gootenberg, Jonathan S, Horii, Takuro, Abudayyeh, Omar O, Kimura, Mika, Hsu, Patrick D, Nakane, Takanori, Ishitani, Ryuichiro, Hatada, Izuho, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 25.02.2016
Predmet:	Animals Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Blastocyst - metabolism CRISPR-Associated Protein 9 CRISPR-Cas Systems Crystallography, X-Ray Embryo, Mammalian - metabolism Endonucleases - chemistry Endonucleases - genetics Endonucleases - metabolism Francisella - enzymology Genetic Engineering - methods Mice Microinjections - methods Models, Molecular RNA, Guide, CRISPR-Cas Systems - genetics
ISSN:	1097-4172, 1097-4172
On-line prístup:	Zistit podrobnosti o prístupe
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