Structure and Engineering of Francisella novicida Cas9

The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing. Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA target sequence, thus constricting the range of targetab...

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Veröffentlicht in:	Cell Jg. 164; H. 5; S. 950
Hauptverfasser:	Hirano, Hisato, Gootenberg, Jonathan S, Horii, Takuro, Abudayyeh, Omar O, Kimura, Mika, Hsu, Patrick D, Nakane, Takanori, Ishitani, Ryuichiro, Hatada, Izuho, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 25.02.2016
Schlagworte:	Animals Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Blastocyst - metabolism CRISPR-Associated Protein 9 CRISPR-Cas Systems Crystallography, X-Ray Embryo, Mammalian - metabolism Endonucleases - chemistry Endonucleases - genetics Endonucleases - metabolism Francisella - enzymology Genetic Engineering - methods Mice Microinjections - methods Models, Molecular RNA, Guide, CRISPR-Cas Systems - genetics
ISSN:	1097-4172, 1097-4172
Online-Zugang:	Weitere Angaben
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