Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida)

BACKGROUND: The 5’ region of cytochrome oxidase I (COI) is the standard marker for DNA barcoding. However, COI has proved to be of limited use in identifying some species, and for some taxa, the coding sequence is not efficiently amplified by PCR. These deficiencies lead to uncertainty as to whether...

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Bibliographic Details
Published in:	Parasites & vectors Vol. 7; no. 1; p. 93
Main Authors:	Lv, Jizhou, Wu, Shaoqiang, Zhang, Yongning, Chen, Yan, Feng, Chunyan, Yuan, Xiangfen, Jia, Guangle, Deng, Junhua, Wang, Caixia, Wang, Qin, Mei, Lin, Lin, Xiangmei
Format:	Journal Article
Language:	English
Published:	London Springer-Verlag 03.03.2014 BioMed Central Springer Nature B.V BMC
Subjects:	16S rDNA Acari Animals Arachnids barcoding Base Sequence Biomedical and Life Sciences Biomedicine COI cytochrome-c oxidase Deoxyribonucleic acid DNA DNA Barcode DNA barcoding DNA Barcoding, Taxonomic DNA Primers - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - genetics Electron Transport Complex IV - genetics Entomology Genes genetic markers Genetic Markers - genetics Genetic Variation Infectious Diseases internal transcribed spacers ITS2 Ixodida Ixodidae Methods Molecular Sequence Data Parasitology Phylogenetics Phylogeny Polymerase Chain Reaction ribosomal DNA Sequence Alignment Sequence Analysis, DNA Species identification Species Specificity Studies Ticks Ticks - classification Ticks - genetics Tropical Medicine uncertainty Veterinary Medicine/Veterinary Science Virology Nearest neighbour 16S rDNA Ticks ITS2 12S rDNA DNA Barcode BLASTn COI Species identification
ISSN:	1756-3305, 1756-3305
Online Access:	Get full text
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