A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry

Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since analytical tissue blanks do not exist for endogenous metabolites, careful consideration must be given to confirm molecular identity....

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Bibliographic Details
Published in:Journal of mass spectrometry. Vol. 49; no. 8; pp. 665 - 673
Main Authors: Manier, M. Lisa, Spraggins, Jeffrey M., Reyzer, Michelle L., Norris, Jeremy L., Caprioli, Richard M.
Format: Journal Article
Language:English
Published: England Blackwell Publishing Ltd 01.08.2014
Wiley Subscription Services, Inc
Subjects:
Acrolein - analogs & derivatives
Adrenal Glands - chemistry
amine metabolites
Amines
Amino acids
Amino Acids - analysis
Amino Acids - chemistry
Animals
Catecholamines - analysis
Catecholamines - chemistry
Cerebellum - chemistry
Chromatography, High Pressure Liquid
Histocytochemistry - methods
identification
Imaging
in-tissue derivatization
Ionization
Liquid chromatography
MALDI imaging
Mass spectrometry
Mathematical analysis
Metabolites
Molecular Imaging - methods
Neurotransmitters
Rats
reagent precoated MALDI targets
Reproducibility of Results
Spatial distribution
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Strategy
Swine
Tissues
identification
neurotransmitters
amino acids
in-tissue derivatization
reagent precoated MALDI targets
MALDI imaging
amine metabolites
ISSN:1076-5174, 1096-9888, 1096-9888
Online Access:Get full text
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Description
Summary:Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since analytical tissue blanks do not exist for endogenous metabolites, careful consideration must be given to confirm molecular identity. Here, we present approaches for the improvement in detection of endogenous amine metabolites such as amino acids and neurotransmitters in tissues through chemical derivatization and matrix‐assisted laser desorption/ionization (MALDI) IMS. Chemical derivatization with 4‐hydroxy‐3‐methoxycinnamaldehyde (CA) was used to improve sensitivity and specificity. CA was applied to the tissue via MALDI sample targets precoated with a mixture of derivatization reagent and ferulic acid as a MALDI matrix. Spatial distributions of chemically derivatized endogenous metabolites in tissue were determined by high‐mass resolution and MSn IMS. We highlight an analytical strategy for metabolite validation whereby tissue extracts are analyzed by high‐performance liquid chromatography (HPLC)‐MS/MS to unambiguously identify metabolites and distinguish them from isobaric compounds. Copyright © 2014 John Wiley & Sons, Ltd.
Bibliography:Supporting info item
ArticleID:JMS3411
istex:8DE52FA288CA84B5559C4386F759FBC8215D186A
National Center for Research Resources - No. GM103391-04
ark:/67375/WNG-8LTCF0WQ-C
NIH/NIGMS - No. 5R01 GM058008-15
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1076-5174
1096-9888
1096-9888
DOI:10.1002/jms.3411