Evaluation of Three Different Forward Primers by Terminal Restriction Fragment Length Polymorphism Analysis for Determination of Fecal Bifidobacterium Spp. in Healthy Subjects

The 27F forward primer is frequently used in 16S rRNA gene libraries and T‐RFLP analysis. However, Bifidobacterium spp. were barely detected with this primer in human fecal samples. In this study, fecal microbiota were analyzed using the T‐RFLP method with three different forward primers (27F, 35F,...

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Veröffentlicht in:	Microbiology and immunology Jg. 48; H. 1; S. 1 - 6
Hauptverfasser:	Hayashi, Hidenori, Sakamoto, Mitsuo, Benno, Yoshimi
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Australia Blackwell Publishing Ltd 01.01.2004
Schlagworte:	Adult Bifidobacterium Bifidobacterium - classification Bifidobacterium - genetics Bifidobacterium - isolation & purification DNA Primers DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification DNA, Ribosomal - analysis DNA, Ribosomal - chemistry DNA, Ribosomal - isolation & purification Feces - microbiology Female forward primer human fecal microbiota Humans Male Middle Aged Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Species Specificity spp T-RFLP
ISSN:	0385-5600, 1348-0421
Online-Zugang:	Volltext
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