An ionising radiation-induced specific transcriptional signature of inflammation-associated genes in whole blood from radiotherapy patients: a pilot study

Background This communication reports the identification of a new panel of transcriptional changes in inflammation-associated genes observed in response to ionising radiation received by radiotherapy patients. Methods Peripheral blood samples were taken with ethical approval and informed consent fro...

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Bibliographic Details
Published in:Radiation oncology (London, England) Vol. 16; no. 1; pp. 83 - 10
Main Authors: Cruz-Garcia, Lourdes, Badie, Christophe, Anbalagan, Selvakumar, Moquet, Jayne, Gothard, Lone, O’Brien, Grainne, Somaiah, Navita, Ainsbury, Elizabeth A.
Format: Journal Article
Language:English
Published: London BioMed Central 03.05.2021
BioMed Central Ltd
Springer Nature B.V
BMC
Subjects:
Antigens
Biomarkers
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Biomedical and Life Sciences
Biomedicine
Blood
Breast
Breast Neoplasms - blood
Breast Neoplasms - genetics
Breast Neoplasms - immunology
Breast Neoplasms - radiotherapy
Cancer
Cancer Research
Cancer therapies
Care and treatment
Chemotherapy
Cytokines
Dosimetry
Female
Gastrointestinal Neoplasms - blood
Gastrointestinal Neoplasms - genetics
Gastrointestinal Neoplasms - immunology
Gastrointestinal Neoplasms - radiotherapy
Gene expression
Gene Expression Regulation, Neoplastic - radiation effects
Genes
Health aspects
Humans
Imaging
Immunity
Inflammation
Inflammation - genetics
Informed consent
Ionising radiation
Ionizing radiation
Lung Neoplasms - blood
Lung Neoplasms - genetics
Lung Neoplasms - immunology
Lung Neoplasms - radiotherapy
Medical research
Multivariate analysis
NCounter
Neoplasms - blood
Neoplasms - genetics
Neoplasms - immunology
Neoplasms - radiotherapy
Nuclear medicine
Oncology
Patient outcomes
Patients
Peripheral blood
Pilot Projects
Prognosis
Radiation Biology and Molecular Radiation Oncology
Radiation effects
Radiation therapy
Radiation, Ionizing
Radiology
Radiotherapy
RT-qPCR
Statistical analysis
Statistical methods
Transcription
Transcriptome - radiation effects
Tumors
Urogenital Neoplasms - blood
Urogenital Neoplasms - genetics
Urogenital Neoplasms - immunology
Urogenital Neoplasms - radiotherapy
Variance analysis
United Kingdom
United Kingdom > UK
United States > US
Germany
Transcription
NCounter
Ionising radiation
Inflammation
Gene expression
Radiotherapy
RT-qPCR
Blood
Cancer
ISSN:1748-717X, 1748-717X
Online Access:Get full text
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Description
Summary:Background This communication reports the identification of a new panel of transcriptional changes in inflammation-associated genes observed in response to ionising radiation received by radiotherapy patients. Methods Peripheral blood samples were taken with ethical approval and informed consent from a total of 20 patients undergoing external beam radiotherapy for breast, lung, gastrointestinal or genitourinary tumours. Nanostring nCounter analysis of transcriptional changes was carried out in samples prior and 24 h post-delivery of the 1st radiotherapy fraction, just prior to the 5th or 6th fraction, and just before the last fraction. Results Statistical analysis with BRB-ArrayTools, GLM MANOVA and nSolver, revealed a radiation responsive panel of genes which varied by patient group (type of cancer) and with time since exposure (as an analogue for dose received), which may be useful as a biomarker of radiation response. Conclusion Further validation in a wider group of patients is ongoing, together with work towards a full understanding of patient specific responses in support of personalised approaches to radiation medicine.
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ISSN:1748-717X
1748-717X
DOI:10.1186/s13014-021-01807-4