A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system

Therapies that target the function of immune cells have significant clinical efficacy in diseases such as cancer and autoimmunity. Although functional genomics has accelerated therapeutic target discovery in cancer, its use in primary immune cells is limited because vector delivery is inefficient an...

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Bibliographic Details
Published in:	Nature communications Vol. 10; no. 1; pp. 1668 - 10
Main Authors:	LaFleur, Martin W., Nguyen, Thao H., Coxe, Matthew A., Yates, Kathleen B., Trombley, Justin D., Weiss, Sarah A., Brown, Flavian D., Gillis, Jacob E., Coxe, Daniel J., Doench, John G., Haining, W. Nicholas, Sharpe, Arlene H.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 10.04.2019 Nature Publishing Group Nature Portfolio
Subjects:	13 13/1 13/100 13/31 42 42/41 49 49/23 631/250/1619 631/250/248 631/250/251 631/250/254 631/250/580 64 64/60 Adaptive Immunity - genetics Adaptive systems Animals Autoimmune diseases Autoimmunity Bone marrow Bone Marrow Transplantation Cancer CD8 antigen CD8-Positive T-Lymphocytes - immunology Cell Line, Tumor Cell Lineage - genetics Cell Lineage - immunology Chlorocebus aethiops Clonal deletion CRISPR CRISPR-Cas Systems - genetics Disease Models, Animal Feasibility Studies Female Gene Transfer Techniques Genes Genetic screening Genetic Testing - methods Genetic Vectors - genetics Genomics Genomics - methods HEK293 Cells Humanities and Social Sciences Humans Immune system Immunity, Innate - genetics Lymphocytes Lymphocytes T Lymphocytic Choriomeningitis - genetics Lymphocytic Choriomeningitis - immunology Lymphocytic Choriomeningitis - virology Lymphocytic choriomeningitis virus - immunology Male Mice Mice, Inbred C57BL Mice, Transgenic multidisciplinary Protein Tyrosine Phosphatase, Non-Receptor Type 2 - genetics Protein Tyrosine Phosphatase, Non-Receptor Type 2 - immunology PTPN2 protein RNA, Guide, CRISPR-Cas Systems - genetics Science Science (multidisciplinary) Screening Therapeutic applications Transplantation Chimera Vero Cells
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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