Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase–quantitative polymerase chain reaction and immuno–quantitative polymerase chain reaction

Laser-assisted microdissection has mainly been used in cancer studies to excise pure cell populations from heterogeneous tissues. Cancer and normal cells selected by laser-assisted microdissection have frequently been used for mRNA expression studies usually by reverse transcriptase–quantitative pol...

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Bibliographic Details
Published in:	Human pathology Vol. 39; no. 10; pp. 1474 - 1482
Main Authors:	Pinzani, Pamela, Lind, Kristina, Malentacchi, Francesca, Nesi, Gabriella, Salvianti, Francesca, Villari, Donata, Kubista, Mikael, Pazzagli, Mario, Orlando, Claudio
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 01.10.2008 Elsevier Elsevier Limited
Subjects:	Acids Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Antibodies, Monoclonal - immunology Biological and medical sciences Cell Line, Tumor Enzyme-Linked Immunosorbent Assay Gene expression Gene Expression Regulation, Neoplastic Humans Hypotheses Immuno-qPCR Investigative techniques, diagnostic techniques (general aspects) Laser microdissection Lasers Male Medical sciences Methods Microdissection Pathology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase chain reaction Polymerase Chain Reaction - methods Prostate - metabolism Prostate - pathology Prostate cancer Prostate specific antigen Prostate-Specific Antigen - genetics Prostate-Specific Antigen - metabolism Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Real time quantitative RT-PCR Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism RNA, Neoplasm - analysis Studies Immuno-qPCR Prostate specific antigen Real time quantitative RT-PCR Laser microdissection Human RNA-directed DNA polymerase Microdissection Enzyme Transferases Tumoral marker Real time Protein Polymerase chain reaction Real time quantitative Nucleotidyltransferases Anatomic pathology Messenger RNA RT-PCR Laser Urogenital system Cell Prostate Reverse transcription polymerase chain reaction Quantitative analysis
ISSN:	0046-8177, 1532-8392, 1532-8392
Online Access:	Get full text
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