Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Genetically-encoded biosensors based on a single fluorescent protein are widely used to visualize analyte levels or enzymatic activities in cells, though usually to monitor relative changes rather than absolute values. We report photochromism-enabled absolute quantification (PEAQ) biosensing, a meth...

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Bibliographic Details
Published in:	Nature communications Vol. 13; no. 1; pp. 1850 - 13
Main Authors:	Bierbuesse, Franziska, Bourges, Anaïs C., Gielen, Vincent, Mönkemöller, Viola, Vandenberg, Wim, Shen, Yi, Hofkens, Johan, Vanden Berghe, Pieter, Campbell, Robert E., Moeyaert, Benjamien, Dedecker, Peter
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 06.04.2022 Nature Publishing Group Nature Portfolio
Subjects:	631/1647/1888/2249 631/1647/245/2225 631/1647/328/2235 639/624/1107/328/2236 639/624/1107/510 Biosensing Techniques - methods Biosensors Calcium ions Dynamical systems Enzymatic activity Fluorescence Fluorescence Resonance Energy Transfer Fluorescent Dyes Humanities and Social Sciences Ionophores Light multidisciplinary Photochromism Proteins Science Science (multidisciplinary)
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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