Context specificity of the EMT transcriptional response

Epithelial–mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial–mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysi...

Full description

Saved in:

Bibliographic Details
Published in:	Nature communications Vol. 11; no. 1; pp. 2142 - 9
Main Authors:	Cook, David P., Vanderhyden, Barbara C.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01.05.2020 Nature Publishing Group Nature Portfolio
Subjects:	38 38/91 45 45/47 49 49/39 631/114/2163 631/67/2329 631/67/322 A549 Cells Biological activity Comparative analysis Context Enzyme inhibitors Epithelial Cells - metabolism Epithelial-Mesenchymal Transition - genetics Epithelial-Mesenchymal Transition - physiology Gene expression Gene Expression Regulation, Neoplastic - genetics Gene Expression Regulation, Neoplastic - physiology Gene sequencing Genes Humanities and Social Sciences Humans Kinases MCF-7 Cells Mesenchyme Modularity multidisciplinary Multiplexing Ribonucleic acid RNA Science Science (multidisciplinary) Sequence Analysis, RNA Signal Transduction - genetics Signal Transduction - physiology Transcription
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Epithelial–mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial–mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysis of the EMT response, leveraging highly multiplexed single-cell RNA sequencing (scRNA-seq) to measure expression profiles of 103,999 cells from 960 samples, comprising 12 EMT time course experiments and independent kinase inhibitor screens for each. We demonstrate that the EMT is vastly context specific, with an average of only 22% of response genes being shared between any two conditions, and over half of all response genes were restricted to 1–2 time course experiments. Further, kinase inhibitor screens revealed signaling dependencies and modularity of these responses. These findings suggest that the EMT is not simply a single, linear process, but is highly variable and modular, warranting quantitative frameworks for understanding nuances of the transition. It is unclear if a common EMT expression program exists. Here, the authors perform multiplexed single-cell RNA sequencing across 12 EMT time courses and 16 kinase inhibitor screens, and find that EMT transcriptional responses are context specific and EMT is not a single, linear transition.
AbstractList	Epithelial-mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial-mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysis of the EMT response, leveraging highly multiplexed single-cell RNA sequencing (scRNA-seq) to measure expression profiles of 103,999 cells from 960 samples, comprising 12 EMT time course experiments and independent kinase inhibitor screens for each. We demonstrate that the EMT is vastly context specific, with an average of only 22% of response genes being shared between any two conditions, and over half of all response genes were restricted to 1-2 time course experiments. Further, kinase inhibitor screens revealed signaling dependencies and modularity of these responses. These findings suggest that the EMT is not simply a single, linear process, but is highly variable and modular, warranting quantitative frameworks for understanding nuances of the transition.Epithelial-mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial-mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysis of the EMT response, leveraging highly multiplexed single-cell RNA sequencing (scRNA-seq) to measure expression profiles of 103,999 cells from 960 samples, comprising 12 EMT time course experiments and independent kinase inhibitor screens for each. We demonstrate that the EMT is vastly context specific, with an average of only 22% of response genes being shared between any two conditions, and over half of all response genes were restricted to 1-2 time course experiments. Further, kinase inhibitor screens revealed signaling dependencies and modularity of these responses. These findings suggest that the EMT is not simply a single, linear process, but is highly variable and modular, warranting quantitative frameworks for understanding nuances of the transition. Epithelial–mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial–mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysis of the EMT response, leveraging highly multiplexed single-cell RNA sequencing (scRNA-seq) to measure expression profiles of 103,999 cells from 960 samples, comprising 12 EMT time course experiments and independent kinase inhibitor screens for each. We demonstrate that the EMT is vastly context specific, with an average of only 22% of response genes being shared between any two conditions, and over half of all response genes were restricted to 1–2 time course experiments. Further, kinase inhibitor screens revealed signaling dependencies and modularity of these responses. These findings suggest that the EMT is not simply a single, linear process, but is highly variable and modular, warranting quantitative frameworks for understanding nuances of the transition. It is unclear if a common EMT expression program exists. Here, the authors perform multiplexed single-cell RNA sequencing across 12 EMT time courses and 16 kinase inhibitor screens, and find that EMT transcriptional responses are context specific and EMT is not a single, linear transition. Epithelial–mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial–mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysis of the EMT response, leveraging highly multiplexed single-cell RNA sequencing (scRNA-seq) to measure expression profiles of 103,999 cells from 960 samples, comprising 12 EMT time course experiments and independent kinase inhibitor screens for each. We demonstrate that the EMT is vastly context specific, with an average of only 22% of response genes being shared between any two conditions, and over half of all response genes were restricted to 1–2 time course experiments. Further, kinase inhibitor screens revealed signaling dependencies and modularity of these responses. These findings suggest that the EMT is not simply a single, linear process, but is highly variable and modular, warranting quantitative frameworks for understanding nuances of the transition. It is unclear if a common EMT expression program exists. Here, the authors perform multiplexed single-cell RNA sequencing across 12 EMT time courses and 16 kinase inhibitor screens, and find that EMT transcriptional responses are context specific and EMT is not a single, linear transition. Epithelial–mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial–mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysis of the EMT response, leveraging highly multiplexed single-cell RNA sequencing (scRNA-seq) to measure expression profiles of 103,999 cells from 960 samples, comprising 12 EMT time course experiments and independent kinase inhibitor screens for each. We demonstrate that the EMT is vastly context specific, with an average of only 22% of response genes being shared between any two conditions, and over half of all response genes were restricted to 1–2 time course experiments. Further, kinase inhibitor screens revealed signaling dependencies and modularity of these responses. These findings suggest that the EMT is not simply a single, linear process, but is highly variable and modular, warranting quantitative frameworks for understanding nuances of the transition. It is unclear if a common EMT expression program exists. Here, the authors perform multiplexed single-cell RNA sequencing across 12 EMT time courses and 16 kinase inhibitor screens, and find that EMT transcriptional responses are context specific and EMT is not a single, linear transition.
ArticleNumber	2142
Author	Cook, David P. Vanderhyden, Barbara C.
Author_xml	– sequence: 1 givenname: David P. orcidid: 0000-0001-7639-6724 surname: Cook fullname: Cook, David P. email: david.cook@uottawa.ca organization: Cancer Therapeutics Program, Ottawa Hospital Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa – sequence: 2 givenname: Barbara C. orcidid: 0000-0002-7644-7189 surname: Vanderhyden fullname: Vanderhyden, Barbara C. email: bvanderhyden@ohri.ca organization: Cancer Therapeutics Program, Ottawa Hospital Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/32358524$$D View this record in MEDLINE/PubMed
BookMark	eNp9kktv1DAUhS1URB_0D7BAkdiwCfj92CChUSmVitiUteU4N1OPMnawMxX993iaFtou6o0t-7tH5_qeY3QQUwSE3hH8iWCmPxdOuFQtprglEkvZ0lfoiGJOWqIoO3h0PkSnpWxwXcwQzfkbdMgoE1pQfoTUKsUZ_sxNmcCHIfgw3zZpaOZraM5-XDVzdrH4HKY5pOjGJkOZUizwFr0e3Fjg9H4_Qb--nV2tvreXP88vVl8vW19Nza1nRnXeD9IojalwxoAeBiokMYZjoIJ1umfUaU-d0bjzgvSVVwII9wYoO0EXi26f3MZOOWxdvrXJBXt3kfLaujwHP4LVhEo_MKWNA656piXD3kjvO-cVZ0PV-rJoTbtuC72HWLsbn4g-fYnh2q7TjVXECC5kFfh4L5DT7x2U2W5D8TCOLkLaFUtrt1JSKXFFPzxDN2mX6w8uFKVSSFOp948d_bPyMJ8K6AXwOZWSYbB1QG4_i2owjJZgu0-DXdJgaxrsXRrs_ufos9IH9ReL2FJUKhzXkP_bfqHqL0_axaM
CitedBy_id	crossref_primary_10_1007_s00018_022_04199_0 crossref_primary_10_1073_pnas_2316733121 crossref_primary_10_1158_0008_5472_CAN_21_4370 crossref_primary_10_3389_fimmu_2025_1639853 crossref_primary_10_1038_s42003_021_01941_5 crossref_primary_10_1158_1541_7786_MCR_22_0509 crossref_primary_10_3389_fgene_2020_604585 crossref_primary_10_1088_1361_6560_ace305 crossref_primary_10_1016_j_ceb_2021_01_001 crossref_primary_10_1186_s13036_023_00333_z crossref_primary_10_3390_cancers14010209 crossref_primary_10_1016_j_bpj_2024_03_021 crossref_primary_10_3390_biom12020297 crossref_primary_10_3390_cancers13246312 crossref_primary_10_1002_advs_202101229 crossref_primary_10_1002_lary_70057 crossref_primary_10_1172_JCI164227 crossref_primary_10_1016_j_yexcr_2024_113993 crossref_primary_10_3390_cancers15051477 crossref_primary_10_1091_mbc_E21_10_0521 crossref_primary_10_3389_fphar_2021_737570 crossref_primary_10_1038_s41598_022_16829_5 crossref_primary_10_1126_scisignal_ado3473 crossref_primary_10_3389_fmolb_2022_807324 crossref_primary_10_1007_s10555_021_09960_8 crossref_primary_10_7554_eLife_61461 crossref_primary_10_1016_j_addr_2020_08_002 crossref_primary_10_1016_j_semcancer_2023_09_007 crossref_primary_10_1038_s41389_025_00560_7 crossref_primary_10_1038_s41592_024_02266_x crossref_primary_10_3389_fonc_2022_912065 crossref_primary_10_3390_cancers13071609 crossref_primary_10_1016_j_isci_2024_110310 crossref_primary_10_1088_1361_6633_acec88 crossref_primary_10_1007_s12038_022_00254_x crossref_primary_10_1038_s41388_021_02128_2 crossref_primary_10_3390_cancers14051138 crossref_primary_10_3390_cancers15020558 crossref_primary_10_3390_ijms21186835 crossref_primary_10_1093_nargab_lqac072 crossref_primary_10_1186_s13059_024_03428_y crossref_primary_10_1242_jcs_261549 crossref_primary_10_1038_s41698_025_00953_2 crossref_primary_10_1038_s42256_023_00763_w crossref_primary_10_1007_s10585_021_10139_2 crossref_primary_10_1016_j_yexcr_2024_113930 crossref_primary_10_1007_s12539_024_00686_z crossref_primary_10_3389_fcell_2024_1259953 crossref_primary_10_31083_j_fbl2704127 crossref_primary_10_1038_s41588_022_01151_7 crossref_primary_10_1002_jcb_30408 crossref_primary_10_7554_eLife_80900 crossref_primary_10_3389_fonc_2022_913803 crossref_primary_10_1038_s41540_025_00512_2 crossref_primary_10_1016_j_xpro_2023_102819 crossref_primary_10_3390_cells13020181 crossref_primary_10_7554_eLife_74866 crossref_primary_10_1186_s12967_023_04102_w crossref_primary_10_1088_1478_3975_ac4ee2 crossref_primary_10_1002_dvg_23591 crossref_primary_10_3390_cancers13205135 crossref_primary_10_1038_s41378_023_00577_1 crossref_primary_10_7554_eLife_76535 crossref_primary_10_3390_ijms252312618 crossref_primary_10_3389_fgene_2021_719099 crossref_primary_10_1038_s41416_020_01213_9 crossref_primary_10_1038_s41467_024_54821_x crossref_primary_10_1038_s41580_021_00442_x crossref_primary_10_1038_s41568_021_00376_8 crossref_primary_10_1371_journal_pcbi_1009193 crossref_primary_10_3390_ijms232112718 crossref_primary_10_1007_s10555_025_10240_y crossref_primary_10_3389_fbioe_2022_829509 crossref_primary_10_3389_fimmu_2021_797261 crossref_primary_10_3390_cells11142208 crossref_primary_10_1016_j_abb_2020_108664 crossref_primary_10_1016_j_vph_2025_107498 crossref_primary_10_1093_bioinformatics_btaf218 crossref_primary_10_1016_j_cell_2024_04_032 crossref_primary_10_1038_s41540_023_00322_4 crossref_primary_10_1172_JCI185423 crossref_primary_10_15252_msb_202211176 crossref_primary_10_1016_j_semcdb_2021_09_010 crossref_primary_10_1158_2159_8290_CD_23_0225 crossref_primary_10_3390_biom10121676 crossref_primary_10_3390_ijms22158334 crossref_primary_10_1038_s41416_020_01150_7 crossref_primary_10_1016_j_tcb_2023_04_004 crossref_primary_10_1186_s12885_024_13057_0 crossref_primary_10_1172_JCI131178 crossref_primary_10_3390_jcm10112403 crossref_primary_10_1007_s10585_022_10146_x crossref_primary_10_1016_j_isci_2024_109888 crossref_primary_10_1038_s42003_024_06441_w crossref_primary_10_3390_biology13020130 crossref_primary_10_1038_s41419_024_07011_y crossref_primary_10_1016_j_canlet_2023_216588 crossref_primary_10_1159_000515289 crossref_primary_10_3389_fonc_2021_686792 crossref_primary_10_1038_s41467_023_36439_7 crossref_primary_10_3390_biom12010029 crossref_primary_10_1038_s42003_024_07408_7 crossref_primary_10_1073_pnas_2406842121 crossref_primary_10_3390_cells14020080 crossref_primary_10_3390_biom12030348 crossref_primary_10_1038_s41540_025_00502_4 crossref_primary_10_1016_j_xinn_2025_101010 crossref_primary_10_1038_s41598_024_83396_2 crossref_primary_10_1016_j_prp_2023_154836 crossref_primary_10_1038_s41586_022_05194_y crossref_primary_10_1038_s41467_022_30362_z crossref_primary_10_1038_s42003_025_08695_4 crossref_primary_10_1093_nar_gkad262 crossref_primary_10_1038_s41576_023_00601_0 crossref_primary_10_3390_cancers16132354 crossref_primary_10_1016_j_synbio_2023_11_010 crossref_primary_10_3389_fcell_2022_1038841 crossref_primary_10_1002_1878_0261_13443 crossref_primary_10_1088_1478_3975_ac482c crossref_primary_10_1172_JCI143762 crossref_primary_10_1038_s41416_021_01689_z crossref_primary_10_3390_ijms241914934 crossref_primary_10_15252_embj_2021109221 crossref_primary_10_1002_cso2_1021 crossref_primary_10_1007_s10555_021_10003_5 crossref_primary_10_1038_s41418_024_01267_9 crossref_primary_10_1089_cell_2023_0095 crossref_primary_10_1016_j_jbc_2021_101032 crossref_primary_10_1371_journal_pcbi_1012762 crossref_primary_10_1098_rsos_220186 crossref_primary_10_3390_cancers13225726 crossref_primary_10_3390_cancers15235694
Cites_doi	10.1038/s41586-018-0590-4 10.1158/1535-7163.MCT-12-1007 10.1038/nmeth.4401 10.1007/s13277-014-2403-1 10.1038/bjc.2011.405 10.1002/path.5155 10.1073/pnas.1812876116 10.1158/0008-5472.CAN-16-3292 10.1158/0008-5472.CAN-16-0246 10.1016/j.devcel.2018.05.027 10.1186/1465-9921-6-56 10.1016/j.cels.2015.12.004 10.1091/mbc.e11-02-0103 10.1096/fj.11-194654 10.1002/0471142727.mb2129s109 10.1016/j.devcel.2019.04.026 10.1038/s41591-018-0096-5 10.1016/j.cell.2016.06.028 10.1093/bioinformatics/btu170 10.1038/nbt.4096 10.1038/s41419-019-1397-4 10.1158/1541-7786.MCR-10-0490 10.1038/s41418-018-0175-7 10.18632/oncotarget.25692 10.1016/j.celrep.2018.10.047 10.1016/S1535-6108(03)00304-0 10.1038/onc.2016.500 10.1038/nmeth.1923 10.1152/ajprenal.00055.2008 10.1016/j.ydbio.2010.05.492 10.1002/mc.22797 10.2147/OTT.S215276 10.1038/s41592-019-0433-8 10.1016/j.cell.2011.04.029 10.1016/j.celrep.2018.07.026 10.1039/C5MB00413F 10.1038/cdd.2014.157 10.1172/JCI200421358 10.1038/nmeth.4463 10.1016/j.canlet.2010.09.007 10.1038/s41588-019-0489-5 10.3892/ijmm.2015.2222 10.1186/gb-2008-9-9-r137 10.18632/oncotarget.1456 10.1038/s41592-019-0689-z 10.3233/CBM-190013 10.15252/embr.201439496 10.1590/S0100-879X2007000800007 10.1016/j.ajpath.2010.12.003 10.1016/j.ccr.2013.04.020 10.1186/1756-8935-6-28 10.1091/mbc.e14-05-1015 10.1038/s41556-018-0062-y 10.1038/s41580-018-0080-4 10.1593/neo.04241 10.1101/622001 10.1101/060012
ContentType	Journal Article
Copyright	The Author(s) 2020 The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml	– notice: The Author(s) 2020 – notice: The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID	C6C AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QL 7QP 7QR 7SN 7SS 7ST 7T5 7T7 7TM 7TO 7X7 7XB 88E 8AO 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABUWG AEUYN AFKRA ARAPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. LK8 M0S M1P M7P P5Z P62 P64 PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS RC3 SOI 7X8 5PM DOA
DOI	10.1038/s41467-020-16066-2
DatabaseName	Springer Nature OA Free Journals CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Environment Abstracts Immunology Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection ProQuest Central Essentials Biological Science Database (Proquest) ProQuest Central Technology collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Biological Science Database ProQuest advanced technologies & aerospace journals ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Genetics Abstracts Environment Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals (DOAJ)
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Natural Science Collection Health & Medical Research Collection Biological Science Collection Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Central (New) ProQuest Medical Library (Alumni) Advanced Technologies & Aerospace Collection ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Entomology Abstracts ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) AIDS and Cancer Research Abstracts ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Immunology Abstracts Environment Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic CrossRef MEDLINE Publicly Available Content Database
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
EISSN	2041-1723
EndPage	9
ExternalDocumentID	oai_doaj_org_article_8126cf3789ae47d38630c96ccbac743f PMC7195456 32358524 10_1038_s41467_020_16066_2
Genre	Research Support, Non-U.S. Gov't Journal Article
GrantInformation_xml	– fundername: Natural Sciences and Engineering Research Council of Canada (Grant #RGPIN 2018-0653.8) – fundername: CIHR – fundername: ;
GroupedDBID	--- 0R~ 39C 3V. 53G 5VS 70F 7X7 88E 8AO 8FE 8FG 8FH 8FI 8FJ AAHBH AAJSJ ABUWG ACGFO ACGFS ACIWK ACMJI ACPRK ACSMW ADBBV ADFRT ADMLS ADRAZ AENEX AEUYN AFKRA AFRAH AHMBA AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AMTXH AOIJS ARAPS ASPBG AVWKF AZFZN BBNVY BCNDV BENPR BGLVJ BHPHI BPHCQ BVXVI C6C CCPQU DIK EBLON EBS EE. EMOBN F5P FEDTE FYUFA GROUPED_DOAJ HCIFZ HMCUK HVGLF HYE HZ~ KQ8 LK8 M1P M48 M7P M~E NAO O9- OK1 P2P P62 PIMPY PQQKQ PROAC PSQYO RNS RNT RNTTT RPM SNYQT SV3 TSG UKHRP AASML AAYXX AFFHD CITATION PHGZM PHGZT PJZUB PPXIY PQGLB CGR CUY CVF ECM EIF NPM 7QL 7QP 7QR 7SN 7SS 7ST 7T5 7T7 7TM 7TO 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. P64 PKEHL PQEST PQUKI PRINS RC3 SOI 7X8 PUEGO 5PM
ID	FETCH-LOGICAL-c606t-c397bccf6978025a99e8ff25619940e253b8d32a8c2a980bc51dbcc75e14c9e23
IEDL.DBID	M7P
ISICitedReferencesCount	160
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000531425700023&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	2041-1723
IngestDate	Tue Oct 14 19:02:55 EDT 2025 Tue Nov 04 01:54:33 EST 2025 Thu Oct 02 10:28:51 EDT 2025 Tue Oct 07 06:46:02 EDT 2025 Thu Apr 03 06:55:14 EDT 2025 Tue Nov 18 21:08:04 EST 2025 Sat Nov 29 06:20:22 EST 2025 Fri Feb 21 02:40:09 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	1
Language	English
License	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c606t-c397bccf6978025a99e8ff25619940e253b8d32a8c2a980bc51dbcc75e14c9e23
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ORCID	0000-0002-7644-7189 0000-0001-7639-6724
OpenAccessLink	https://www.proquest.com/docview/2397226569?pq-origsite=%requestingapplication%
PMID	32358524
PQID	2397226569
PQPubID	546298
PageCount	9
ParticipantIDs	doaj_primary_oai_doaj_org_article_8126cf3789ae47d38630c96ccbac743f pubmedcentral_primary_oai_pubmedcentral_nih_gov_7195456 proquest_miscellaneous_2397662660 proquest_journals_2397226569 pubmed_primary_32358524 crossref_citationtrail_10_1038_s41467_020_16066_2 crossref_primary_10_1038_s41467_020_16066_2 springer_journals_10_1038_s41467_020_16066_2
PublicationCentury	2000
PublicationDate	2020-05-01
PublicationDateYYYYMMDD	2020-05-01
PublicationDate_xml	– month: 05 year: 2020 text: 2020-05-01 day: 01
PublicationDecade	2020
PublicationPlace	London
PublicationPlace_xml	– name: London – name: England
PublicationTitle	Nature communications
PublicationTitleAbbrev	Nat Commun
PublicationTitleAlternate	Nat Commun
PublicationYear	2020
Publisher	Nature Publishing Group UK Nature Publishing Group Nature Portfolio
Publisher_xml	– name: Nature Publishing Group UK – name: Nature Publishing Group – name: Nature Portfolio
References	Buenrostro, Wu, Chang, Greenleaf (CR53) 2015; 109 Yao (CR36) 2019; 26 Sun, Schaar, Sukumaran, Dhasarathy, Singh (CR16) 2018; 57 Li, Lin, Huang, Pan, Wang (CR45) 2019; 12 Aiello (CR8) 2018; 45 Yu (CR26) 2014; 35 Devaraj, Bose (CR21) 2019; 8 Tiwari (CR31) 2013; 23 Tarcic (CR47) 2012; 26 Limame (CR28) 2014; 5 Lecharpentier (CR5) 2011; 105 Chen (CR44) 2020; 17 Yonekawa (CR46) 2015; 16 Bocci, Jolly, George, Levine, Onuchic (CR4) 2018; 9 Nieto, Huang, Jackson, Thiery (CR1) 2016; 166 Peixoto (CR10) 2019; 10 Xie (CR42) 2004; 6 Su (CR27) 2016; 76 McGinnis (CR13) 2019; 16 Holian (CR32) 2008; 295 Scheel (CR38) 2011; 145 Puram (CR9) 2017; 171 Yeh (CR39) 2018; 20 Bolger, Lohse, Usadel (CR48) 2014; 30 (CR59) 2018; 562 Dalmau, Jaumot, Tauler, Bedia (CR18) 2015; 11 Derynck, Weinberg (CR2) 2019; 49 Kasai, Allen, Mason, Kamimura, Zhang (CR14) 2005; 6 Butler, Hoffman, Smibert, Papalexi, Satija (CR49) 2018; 36 Langmead, Salzberg (CR54) 2012; 9 Zhang (CR55) 2008; 9 Armstrong (CR7) 2011; 9 Aibar (CR52) 2017; 14 Huber (CR29) 2004; 114 CR51 CR50 Lambrechts (CR57) 2018; 24 McFaline-Figueroa (CR11) 2019; 51 Kröger (CR3) 2019; 116 Schep, Wu, Buenrostro, Greenleaf (CR56) 2017; 14 Principe (CR43) 2017; 36 Gondkar (CR33) 2019; 25 Dongre, Weinberg (CR37) 2019; 20 Larocca (CR40) 2013; 12 Bakiri (CR25) 2015; 22 Suzuki, Osumi, Wakamatsu (CR30) 2010; 344 Kumar (CR58) 2018; 25 Li (CR15) 2015; 36 Lu, Ghosh, Wang, Hunter (CR17) 2003; 4 Liberzon (CR22) 2015; 1 Ward (CR34) 2018; 24 Gemmill (CR12) 2011; 300 Hou (CR6) 2011; 178 Cieślik (CR24) 2013; 6 Dongre (CR35) 2017; 77 McCorry, Loughrey, Longley, Lawler, Dunne (CR23) 2018; 246 Dong (CR19) 2007; 40 Osborne (CR20) 2014; 25 Gregory (CR41) 2011; 22 WS Chen (16066_CR44) 2020; 17 T Suzuki (16066_CR30) 2010; 344 RM Gemmill (16066_CR12) 2011; 300 M Cieślik (16066_CR24) 2013; 6 L Bakiri (16066_CR25) 2015; 22 AM Bolger (16066_CR48) 2014; 30 V Devaraj (16066_CR21) 2019; 8 W Yu (16066_CR26) 2014; 35 J Holian (16066_CR32) 2008; 295 PA Gregory (16066_CR41) 2011; 22 MP Kumar (16066_CR58) 2018; 25 C Ward (16066_CR34) 2018; 24 B Langmead (16066_CR54) 2012; 9 D Lambrechts (16066_CR57) 2018; 24 A Dongre (16066_CR35) 2017; 77 R Limame (16066_CR28) 2014; 5 16066_CR51 16066_CR50 A Dongre (16066_CR37) 2019; 20 T Yonekawa (16066_CR46) 2015; 16 JL McFaline-Figueroa (16066_CR11) 2019; 51 Tabula Muris Consortium. (16066_CR59) 2018; 562 H-W Yeh (16066_CR39) 2018; 20 K Gondkar (16066_CR33) 2019; 25 AJ Armstrong (16066_CR7) 2011; 9 MA Huber (16066_CR29) 2004; 114 L Xie (16066_CR42) 2004; 6 MA Nieto (16066_CR1) 2016; 166 N Tiwari (16066_CR31) 2013; 23 SV Puram (16066_CR9) 2017; 171 AM McCorry (16066_CR23) 2018; 246 C Scheel (16066_CR38) 2011; 145 C Kröger (16066_CR3) 2019; 116 CS McGinnis (16066_CR13) 2019; 16 R Derynck (16066_CR2) 2019; 49 Y Sun (16066_CR16) 2018; 57 C Larocca (16066_CR40) 2013; 12 LD Osborne (16066_CR20) 2014; 25 AN Schep (16066_CR56) 2017; 14 JD Buenrostro (16066_CR53) 2015; 109 S Aibar (16066_CR52) 2017; 14 P Peixoto (16066_CR10) 2019; 10 H Kasai (16066_CR14) 2005; 6 A Butler (16066_CR49) 2018; 36 N Dalmau (16066_CR18) 2015; 11 G Tarcic (16066_CR47) 2012; 26 L Yao (16066_CR36) 2019; 26 J-M Hou (16066_CR6) 2011; 178 A Liberzon (16066_CR22) 2015; 1 L Su (16066_CR27) 2016; 76 NM Aiello (16066_CR8) 2018; 45 A Lecharpentier (16066_CR5) 2011; 105 Y Zhang (16066_CR55) 2008; 9 Z Lu (16066_CR17) 2003; 4 DR Principe (16066_CR43) 2017; 36 L Li (16066_CR15) 2015; 36 F Bocci (16066_CR4) 2018; 9 R Dong (16066_CR19) 2007; 40 C-Z Li (16066_CR45) 2019; 12
References_xml	– volume: 562 start-page: 367 year: 2018 end-page: 372 ident: CR59 article-title: Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris publication-title: Nature doi: 10.1038/s41586-018-0590-4 – volume: 12 start-page: 1805 year: 2013 end-page: 1815 ident: CR40 article-title: An autocrine loop between TGF-β1 and the transcription factor brachyury controls the transition of human carcinoma cells into a mesenchymal phenotype publication-title: Mol. Cancer Ther. doi: 10.1158/1535-7163.MCT-12-1007 – volume: 14 start-page: 975 year: 2017 end-page: 978 ident: CR56 article-title: chromVAR: inferring transcription-factor-associated accessibility from single-cell epigenomic data publication-title: Nat. Methods doi: 10.1038/nmeth.4401 – volume: 35 start-page: 10943 year: 2014 end-page: 10951 ident: CR26 article-title: MEF2 transcription factors promotes EMT and invasiveness of hepatocellular carcinoma through TGF-β1 autoregulation circuitry publication-title: Tumour Biol. doi: 10.1007/s13277-014-2403-1 – volume: 105 start-page: 1338 year: 2011 end-page: 1341 ident: CR5 article-title: Detection of circulating tumour cells with a hybrid (epithelial/mesenchymal) phenotype in patients with metastatic non-small cell lung cancer publication-title: Br. J. Cancer doi: 10.1038/bjc.2011.405 – ident: CR51 – volume: 8 start-page: 911 year: 2019 ident: CR21 article-title: Morphological state transition dynamics in egf-induced epithelial to mesenchymal transition publication-title: J. Clin. Med. Res – volume: 246 start-page: 422 year: 2018 end-page: 426 ident: CR23 article-title: Epithelial-to-mesenchymal transition signature assessment in colorectal cancer quantifies tumour stromal content rather than true transition publication-title: J. Pathol. doi: 10.1002/path.5155 – volume: 116 start-page: 7353 year: 2019 end-page: 7362 ident: CR3 article-title: Acquisition of a hybrid E/M state is essential for tumorigenicity of basal breast cancer cells publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1812876116 – volume: 77 start-page: 3982 year: 2017 end-page: 3989 ident: CR35 article-title: Epithelial-to-mesenchymal transition contributes to immunosuppression in breast carcinomas publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-16-3292 – volume: 76 start-page: 5054 year: 2016 end-page: 5067 ident: CR27 article-title: MEF2D transduces microenvironment stimuli to ZEB1 to promote epithelial-mesenchymal transition and metastasis in colorectal cancer publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-16-0246 – volume: 45 start-page: 681 year: 2018 end-page: 695.e4 ident: CR8 article-title: EMT subtype influences epithelial plasticity and mode of cell migration publication-title: Dev. Cell doi: 10.1016/j.devcel.2018.05.027 – volume: 6 start-page: 56 year: 2005 ident: CR14 article-title: TGF-β1 induces human alveolar epithelial to mesenchymal cell transition (EMT) publication-title: Respir. Res. doi: 10.1186/1465-9921-6-56 – volume: 1 start-page: 417 year: 2015 end-page: 425 ident: CR22 article-title: The Molecular Signatures Database (MSigDB) hallmark gene set collection publication-title: Cell Syst. doi: 10.1016/j.cels.2015.12.004 – volume: 22 start-page: 1686 year: 2011 end-page: 1698 ident: CR41 article-title: An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition publication-title: Mol. Biol. Cell doi: 10.1091/mbc.e11-02-0103 – volume: 26 start-page: 1582 year: 2012 end-page: 1592 ident: CR47 article-title: EGR1 and the ERK-ERF axis drive mammary cell migration in response to EGF publication-title: FASEB J. doi: 10.1096/fj.11-194654 – volume: 109 start-page: 21.29.1 year: 2015 end-page: 9 ident: CR53 article-title: ATAC-seq: a method for assaying chromatin accessibility genome-wide publication-title: Curr. Protoc. Mol. Biol. doi: 10.1002/0471142727.mb2129s109 – volume: 49 start-page: 313 year: 2019 end-page: 316 ident: CR2 article-title: EMT and cancer: more than meets the eye publication-title: Dev. Cell doi: 10.1016/j.devcel.2019.04.026 – volume: 24 start-page: 1277 year: 2018 end-page: 1289 ident: CR57 article-title: Phenotype molding of stromal cells in the lung tumor microenvironment publication-title: Nat. Med. doi: 10.1038/s41591-018-0096-5 – volume: 166 start-page: 21 year: 2016 end-page: 45 ident: CR1 article-title: EMT: 2016 publication-title: Cell doi: 10.1016/j.cell.2016.06.028 – volume: 30 start-page: 2114 year: 2014 end-page: 2120 ident: CR48 article-title: Trimmomatic: a flexible trimmer for Illumina sequence data publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu170 – volume: 36 start-page: 411 year: 2018 end-page: 420 ident: CR49 article-title: Integrating single-cell transcriptomic data across different conditions, technologies, and species publication-title: Nat. Biotechnol. doi: 10.1038/nbt.4096 – volume: 10 year: 2019 ident: CR10 article-title: EMT is associated with an epigenetic signature of ECM remodeling genes publication-title: Cell Death Dis. doi: 10.1038/s41419-019-1397-4 – volume: 9 start-page: 997 year: 2011 end-page: 1007 ident: CR7 article-title: Circulating tumor cells from patients with advanced prostate and breast cancer display both epithelial and mesenchymal markers publication-title: Mol. Cancer Res. doi: 10.1158/1541-7786.MCR-10-0490 – ident: CR50 – volume: 26 start-page: 943 year: 2019 end-page: 957 ident: CR36 article-title: Paracrine signalling during ZEB1-mediated epithelial–mesenchymal transition augments local myofibroblast differentiation in lung fibrosis publication-title: Cell Death Differ. doi: 10.1038/s41418-018-0175-7 – volume: 9 start-page: 29906 year: 2018 end-page: 29920 ident: CR4 article-title: A mechanism-based computational model to capture the interconnections among epithelial-mesenchymal transition, cancer stem cells and Notch-Jagged signaling publication-title: Oncotarget doi: 10.18632/oncotarget.25692 – volume: 25 start-page: 1458 year: 2018 end-page: 1468.e4 ident: CR58 article-title: Analysis of single-cell rna-seq identifies cell-cell communication associated with tumor characteristics publication-title: Cell Rep. doi: 10.1016/j.celrep.2018.10.047 – volume: 171 start-page: 1611 year: 2017 end-page: 1624.e24 ident: CR9 article-title: Single-cell transcriptomic analysis of primary and metastatic tumor ecosystems in head and neck publication-title: Cancer Cell – volume: 4 start-page: 499 year: 2003 end-page: 515 ident: CR17 article-title: Downregulation of caveolin-1 function by EGF leads to the loss of E-cadherin, increased transcriptional activity of beta-catenin, and enhanced tumor cell invasion publication-title: Cancer Cell doi: 10.1016/S1535-6108(03)00304-0 – volume: 36 start-page: 4336 year: 2017 end-page: 4348 ident: CR43 article-title: TGFβ engages MEK/ERK to differentially regulate benign and malignant pancreas cell function publication-title: Oncogene doi: 10.1038/onc.2016.500 – volume: 9 start-page: 357 year: 2012 end-page: 359 ident: CR54 article-title: Fast gapped-read alignment with Bowtie 2 publication-title: Nat. Methods doi: 10.1038/nmeth.1923 – volume: 295 start-page: F1388 year: 2008 end-page: F1396 ident: CR32 article-title: Role of Kruppel-like factor 6 in transforming growth factor-beta1-induced epithelial-mesenchymal transition of proximal tubule cells publication-title: Am. J. Physiol. Ren. Physiol. doi: 10.1152/ajprenal.00055.2008 – volume: 344 start-page: 658 year: 2010 end-page: 668 ident: CR30 article-title: Stabilization of ATF4 protein is required for the regulation of epithelial-mesenchymal transition of the avian neural crest publication-title: Dev. Biol. doi: 10.1016/j.ydbio.2010.05.492 – volume: 57 start-page: 752 year: 2018 end-page: 761 ident: CR16 article-title: TGFβ-induced epithelial-to-mesenchymal transition in prostate cancer cells is mediated via TRPM7 expression publication-title: Mol. Carcinogenesis doi: 10.1002/mc.22797 – volume: 12 start-page: 9029 year: 2019 end-page: 9040 ident: CR45 article-title: Receptor-interacting protein kinase 1 promotes cholangiocarcinoma proliferation and lymphangiogenesis through the activation protein 1 pathway publication-title: Onco. Targets Ther. doi: 10.2147/OTT.S215276 – volume: 16 start-page: 619 year: 2019 end-page: 626 ident: CR13 article-title: MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices publication-title: Nat. Methods doi: 10.1038/s41592-019-0433-8 – volume: 145 start-page: 926 year: 2011 end-page: 940 ident: CR38 article-title: Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast publication-title: Cell doi: 10.1016/j.cell.2011.04.029 – volume: 24 start-page: 1496 year: 2018 end-page: 1511.e8 ident: CR34 article-title: Fine-tuning Mybl2 is required for proper mesenchymal-to-epithelial transition during somatic reprogramming publication-title: Cell Rep. doi: 10.1016/j.celrep.2018.07.026 – volume: 11 start-page: 3397 year: 2015 end-page: 3406 ident: CR18 article-title: Epithelial-to-mesenchymal transition involves triacylglycerol accumulation in DU145 prostate cancer cells publication-title: Mol. Biosyst. doi: 10.1039/C5MB00413F – volume: 22 start-page: 336 year: 2015 end-page: 350 ident: CR25 article-title: Fra-1/AP-1 induces EMT in mammary epithelial cells by modulating Zeb1/2 and TGFβ expression publication-title: Cell Death Differ. doi: 10.1038/cdd.2014.157 – volume: 114 start-page: 569 year: 2004 end-page: 581 ident: CR29 article-title: NF-κB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression publication-title: J. Clin. Investig. doi: 10.1172/JCI200421358 – volume: 14 start-page: 1083 year: 2017 end-page: 1086 ident: CR52 article-title: SCENIC: single-cell regulatory network inference and clustering publication-title: Nat. Methods doi: 10.1038/nmeth.4463 – volume: 300 start-page: 66 year: 2011 end-page: 78 ident: CR12 article-title: ZEB1-responsive genes in non-small cell lung cancer publication-title: Cancer Lett. doi: 10.1016/j.canlet.2010.09.007 – volume: 51 start-page: 1389 year: 2019 end-page: 1398 ident: CR11 article-title: A pooled single-cell genetic screen identifies regulatory checkpoints in the continuum of the epithelial-to-mesenchymal transition publication-title: Nat. Genet. doi: 10.1038/s41588-019-0489-5 – volume: 36 start-page: 113 year: 2015 ident: CR15 article-title: Transforming growth factor-β1 induces EMT by the transactivation of epidermal growth factor signaling through HA/CD44 in lung and breast cancer cells publication-title: Int. J. Mol. Med. doi: 10.3892/ijmm.2015.2222 – volume: 9 year: 2008 ident: CR55 article-title: Model-based analysis of ChIP-Seq (MACS) publication-title: Genome Biol. doi: 10.1186/gb-2008-9-9-r137 – volume: 5 start-page: 29 year: 2014 end-page: 48 ident: CR28 article-title: factors in cancer progression: three fingers on the steering wheel publication-title: Oncotarget doi: 10.18632/oncotarget.1456 – volume: 17 start-page: 302 year: 2020 end-page: 310 ident: CR44 article-title: Uncovering axes of variation among single-cell cancer specimens publication-title: Nat. Methods doi: 10.1038/s41592-019-0689-z – volume: 25 start-page: 223 year: 2019 end-page: 232 ident: CR33 article-title: E74 like ETS transcription factor 3 (ELF3) is a negative regulator of epithelial- mesenchymal transition in bladder carcinoma publication-title: Cancer Biomark. doi: 10.3233/CBM-190013 – volume: 16 start-page: 700 year: 2015 end-page: 708 ident: CR46 article-title: RIP1 negatively regulates basal autophagic flux through TFEB to control sensitivity to apoptosis publication-title: EMBO Rep. doi: 10.15252/embr.201439496 – volume: 40 start-page: 1071 year: 2007 end-page: 1078 ident: CR19 article-title: Role of nuclear factor kappa B and reactive oxygen species in the tumor necrosis factor-alpha-induced epithelial-mesenchymal transition of MCF-7 cells publication-title: Braz. J. Med. Biol. Res. doi: 10.1590/S0100-879X2007000800007 – volume: 178 start-page: 989 year: 2011 end-page: 996 ident: CR6 article-title: Circulating tumor cells as a window on metastasis biology in lung cancer publication-title: Am. J. Pathol. doi: 10.1016/j.ajpath.2010.12.003 – volume: 23 start-page: 768 year: 2013 end-page: 783 ident: CR31 article-title: Sox4 is a master regulator of epithelial-mesenchymal transition by controlling Ezh2 expression and epigenetic reprogramming publication-title: Cancer Cell doi: 10.1016/j.ccr.2013.04.020 – volume: 6 start-page: 28 year: 2013 ident: CR24 article-title: Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition publication-title: Epigenetics Chromatin doi: 10.1186/1756-8935-6-28 – volume: 25 start-page: 3528 year: 2014 ident: CR20 article-title: TGF-β regulates LARG and GEF-H1 during EMT to affect stiffening response to force and cell invasion publication-title: Mol. Biol. Cell doi: 10.1091/mbc.e14-05-1015 – volume: 20 start-page: 479 year: 2018 end-page: 491 ident: CR39 article-title: PSPC1 mediates TGF-β1 autocrine signalling and Smad2/3 target switching to promote EMT, stemness and metastasis publication-title: Nat. Cell Biol. doi: 10.1038/s41556-018-0062-y – volume: 20 start-page: 69 year: 2019 end-page: 84 ident: CR37 article-title: New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/s41580-018-0080-4 – volume: 6 start-page: 603 year: 2004 end-page: 610 ident: CR42 article-title: Activation of the Erk Pathway Is Required for TGF-β1-Induced EMT In Vitro publication-title: Neoplasia doi: 10.1593/neo.04241 – volume: 9 start-page: 357 year: 2012 ident: 16066_CR54 publication-title: Nat. Methods doi: 10.1038/nmeth.1923 – volume: 51 start-page: 1389 year: 2019 ident: 16066_CR11 publication-title: Nat. Genet. doi: 10.1038/s41588-019-0489-5 – volume: 6 start-page: 56 year: 2005 ident: 16066_CR14 publication-title: Respir. Res. doi: 10.1186/1465-9921-6-56 – volume: 20 start-page: 479 year: 2018 ident: 16066_CR39 publication-title: Nat. Cell Biol. doi: 10.1038/s41556-018-0062-y – volume: 26 start-page: 943 year: 2019 ident: 16066_CR36 publication-title: Cell Death Differ. doi: 10.1038/s41418-018-0175-7 – volume: 57 start-page: 752 year: 2018 ident: 16066_CR16 publication-title: Mol. Carcinogenesis doi: 10.1002/mc.22797 – volume: 178 start-page: 989 year: 2011 ident: 16066_CR6 publication-title: Am. J. Pathol. doi: 10.1016/j.ajpath.2010.12.003 – volume: 6 start-page: 28 year: 2013 ident: 16066_CR24 publication-title: Epigenetics Chromatin doi: 10.1186/1756-8935-6-28 – volume: 295 start-page: F1388 year: 2008 ident: 16066_CR32 publication-title: Am. J. Physiol. Ren. Physiol. doi: 10.1152/ajprenal.00055.2008 – volume: 562 start-page: 367 year: 2018 ident: 16066_CR59 publication-title: Nature doi: 10.1038/s41586-018-0590-4 – volume: 9 year: 2008 ident: 16066_CR55 publication-title: Genome Biol. doi: 10.1186/gb-2008-9-9-r137 – volume: 9 start-page: 997 year: 2011 ident: 16066_CR7 publication-title: Mol. Cancer Res. doi: 10.1158/1541-7786.MCR-10-0490 – volume: 9 start-page: 29906 year: 2018 ident: 16066_CR4 publication-title: Oncotarget doi: 10.18632/oncotarget.25692 – volume: 17 start-page: 302 year: 2020 ident: 16066_CR44 publication-title: Nat. Methods doi: 10.1038/s41592-019-0689-z – volume: 5 start-page: 29 year: 2014 ident: 16066_CR28 publication-title: Oncotarget doi: 10.18632/oncotarget.1456 – volume: 24 start-page: 1496 year: 2018 ident: 16066_CR34 publication-title: Cell Rep. doi: 10.1016/j.celrep.2018.07.026 – volume: 22 start-page: 336 year: 2015 ident: 16066_CR25 publication-title: Cell Death Differ. doi: 10.1038/cdd.2014.157 – volume: 22 start-page: 1686 year: 2011 ident: 16066_CR41 publication-title: Mol. Biol. Cell doi: 10.1091/mbc.e11-02-0103 – volume: 36 start-page: 411 year: 2018 ident: 16066_CR49 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.4096 – volume: 8 start-page: 911 year: 2019 ident: 16066_CR21 publication-title: J. Clin. Med. Res – volume: 20 start-page: 69 year: 2019 ident: 16066_CR37 publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/s41580-018-0080-4 – volume: 16 start-page: 619 year: 2019 ident: 16066_CR13 publication-title: Nat. Methods doi: 10.1038/s41592-019-0433-8 – volume: 10 year: 2019 ident: 16066_CR10 publication-title: Cell Death Dis. doi: 10.1038/s41419-019-1397-4 – volume: 14 start-page: 975 year: 2017 ident: 16066_CR56 publication-title: Nat. Methods doi: 10.1038/nmeth.4401 – volume: 45 start-page: 681 year: 2018 ident: 16066_CR8 publication-title: Dev. Cell doi: 10.1016/j.devcel.2018.05.027 – volume: 6 start-page: 603 year: 2004 ident: 16066_CR42 publication-title: Neoplasia doi: 10.1593/neo.04241 – volume: 25 start-page: 1458 year: 2018 ident: 16066_CR58 publication-title: Cell Rep. doi: 10.1016/j.celrep.2018.10.047 – volume: 76 start-page: 5054 year: 2016 ident: 16066_CR27 publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-16-0246 – volume: 300 start-page: 66 year: 2011 ident: 16066_CR12 publication-title: Cancer Lett. doi: 10.1016/j.canlet.2010.09.007 – volume: 49 start-page: 313 year: 2019 ident: 16066_CR2 publication-title: Dev. Cell doi: 10.1016/j.devcel.2019.04.026 – volume: 246 start-page: 422 year: 2018 ident: 16066_CR23 publication-title: J. Pathol. doi: 10.1002/path.5155 – volume: 114 start-page: 569 year: 2004 ident: 16066_CR29 publication-title: J. Clin. Investig. doi: 10.1172/JCI200421358 – volume: 145 start-page: 926 year: 2011 ident: 16066_CR38 publication-title: Cell doi: 10.1016/j.cell.2011.04.029 – volume: 166 start-page: 21 year: 2016 ident: 16066_CR1 publication-title: Cell doi: 10.1016/j.cell.2016.06.028 – volume: 25 start-page: 3528 year: 2014 ident: 16066_CR20 publication-title: Mol. Biol. Cell doi: 10.1091/mbc.e14-05-1015 – volume: 4 start-page: 499 year: 2003 ident: 16066_CR17 publication-title: Cancer Cell doi: 10.1016/S1535-6108(03)00304-0 – volume: 25 start-page: 223 year: 2019 ident: 16066_CR33 publication-title: Cancer Biomark. doi: 10.3233/CBM-190013 – ident: 16066_CR50 doi: 10.1101/622001 – volume: 105 start-page: 1338 year: 2011 ident: 16066_CR5 publication-title: Br. J. Cancer doi: 10.1038/bjc.2011.405 – volume: 16 start-page: 700 year: 2015 ident: 16066_CR46 publication-title: EMBO Rep. doi: 10.15252/embr.201439496 – volume: 12 start-page: 1805 year: 2013 ident: 16066_CR40 publication-title: Mol. Cancer Ther. doi: 10.1158/1535-7163.MCT-12-1007 – volume: 171 start-page: 1611 year: 2017 ident: 16066_CR9 publication-title: Cancer Cell – volume: 11 start-page: 3397 year: 2015 ident: 16066_CR18 publication-title: Mol. Biosyst. doi: 10.1039/C5MB00413F – ident: 16066_CR51 doi: 10.1101/060012 – volume: 1 start-page: 417 year: 2015 ident: 16066_CR22 publication-title: Cell Syst. doi: 10.1016/j.cels.2015.12.004 – volume: 36 start-page: 4336 year: 2017 ident: 16066_CR43 publication-title: Oncogene doi: 10.1038/onc.2016.500 – volume: 30 start-page: 2114 year: 2014 ident: 16066_CR48 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu170 – volume: 344 start-page: 658 year: 2010 ident: 16066_CR30 publication-title: Dev. Biol. doi: 10.1016/j.ydbio.2010.05.492 – volume: 109 start-page: 21.29.1 year: 2015 ident: 16066_CR53 publication-title: Curr. Protoc. Mol. Biol. doi: 10.1002/0471142727.mb2129s109 – volume: 40 start-page: 1071 year: 2007 ident: 16066_CR19 publication-title: Braz. J. Med. Biol. Res. doi: 10.1590/S0100-879X2007000800007 – volume: 14 start-page: 1083 year: 2017 ident: 16066_CR52 publication-title: Nat. Methods doi: 10.1038/nmeth.4463 – volume: 23 start-page: 768 year: 2013 ident: 16066_CR31 publication-title: Cancer Cell doi: 10.1016/j.ccr.2013.04.020 – volume: 116 start-page: 7353 year: 2019 ident: 16066_CR3 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1812876116 – volume: 24 start-page: 1277 year: 2018 ident: 16066_CR57 publication-title: Nat. Med. doi: 10.1038/s41591-018-0096-5 – volume: 77 start-page: 3982 year: 2017 ident: 16066_CR35 publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-16-3292 – volume: 36 start-page: 113 year: 2015 ident: 16066_CR15 publication-title: Int. J. Mol. Med. doi: 10.3892/ijmm.2015.2222 – volume: 12 start-page: 9029 year: 2019 ident: 16066_CR45 publication-title: Onco. Targets Ther. doi: 10.2147/OTT.S215276 – volume: 26 start-page: 1582 year: 2012 ident: 16066_CR47 publication-title: FASEB J. doi: 10.1096/fj.11-194654 – volume: 35 start-page: 10943 year: 2014 ident: 16066_CR26 publication-title: Tumour Biol. doi: 10.1007/s13277-014-2403-1
SSID	ssj0000391844
Score	2.650217
Snippet	Epithelial–mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial–mesenchymal transition (EMT)... Epithelial-mesenchymal plasticity contributes to many biological processes, including tumor progression. Various epithelial-mesenchymal transition (EMT)... It is unclear if a common EMT expression program exists. Here, the authors perform multiplexed single-cell RNA sequencing across 12 EMT time courses and 16...
SourceID	doaj pubmedcentral proquest pubmed crossref springer
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher
StartPage	2142
SubjectTerms	38 38/91 45 45/47 49 49/39 631/114/2163 631/67/2329 631/67/322 A549 Cells Biological activity Comparative analysis Context Enzyme inhibitors Epithelial Cells - metabolism Epithelial-Mesenchymal Transition - genetics Epithelial-Mesenchymal Transition - physiology Gene expression Gene Expression Regulation, Neoplastic - genetics Gene Expression Regulation, Neoplastic - physiology Gene sequencing Genes Humanities and Social Sciences Humans Kinases MCF-7 Cells Mesenchyme Modularity multidisciplinary Multiplexing Ribonucleic acid RNA Science Science (multidisciplinary) Sequence Analysis, RNA Signal Transduction - genetics Signal Transduction - physiology Transcription
SummonAdditionalLinks	– databaseName: Directory of Open Access Journals (DOAJ) dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwEB6hqkhcUHmntChI3CBqYjt-HGnVigNUHIrUm-X4ISqhLNpNEfx7Zuzs0uV54ZrY1ujz2PNZnvkM8IIjS2atdo0cvG5ET8XKPqVGDBhNBpM64XOh8Ft1fq4vL837G099UU5YkQcuwB1hF-kTV9q4KFTAsXnrjfR-cB6jX6Ldt1XmxmEq78Hc4NFFzFUyLddHK5H3BDotdUTaG7YVibJg_-9Y5q_Jkj_dmOZAdLYHd2cGWb8ult-DW3G8D7fLm5LfHoDKelNfp5pqKCkPCGl2vUg1Er369N1FPVFwWm8VOM6yJMnGh_Dh7PTi5E0zv47QeLR_ajwyicH7JElDiPXOmKhTQgZDar9tZD0fdODMac-c0e3g-y5ge9VHxN9Exh_BzrgY4xOouQ4BmVtS0XQiBCohlJ5r4VwXTAyigm6NlPWzdDi9YPHJ5itsrm1B1yK6NqNrWQUvN30-F-GMv7Y-pgnYtCTR6_wBXcHOrmD_5QoVHKynz84rcWUZwoQUs5emgueb37iG6GLEjXFxXdpIPNnJtoLHZbY3lnCqJe4ZYqC2_GDL1O0_49XHrNOtSE2vlxW8WnvMD7P-DMX-_4DiKdxh5Oo5M_MAdqbldTyEXf9lulotn-W18h3HahT7 priority: 102 providerName: Directory of Open Access Journals
Title	Context specificity of the EMT transcriptional response
URI	https://link.springer.com/article/10.1038/s41467-020-16066-2 https://www.ncbi.nlm.nih.gov/pubmed/32358524 https://www.proquest.com/docview/2397226569 https://www.proquest.com/docview/2397662660 https://pubmed.ncbi.nlm.nih.gov/PMC7195456 https://doaj.org/article/8126cf3789ae47d38630c96ccbac743f
Volume	11
WOSCitedRecordID	wos000531425700023&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2041-1723 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000391844 issn: 2041-1723 databaseCode: DOA dateStart: 20150101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2041-1723 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000391844 issn: 2041-1723 databaseCode: M~E dateStart: 20100101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 2041-1723 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000391844 issn: 2041-1723 databaseCode: P5Z dateStart: 20100101 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2041-1723 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000391844 issn: 2041-1723 databaseCode: M7P dateStart: 20100101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 2041-1723 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000391844 issn: 2041-1723 databaseCode: 7X7 dateStart: 20100101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central (subscription) customDbUrl: eissn: 2041-1723 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000391844 issn: 2041-1723 databaseCode: BENPR dateStart: 20100101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2041-1723 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000391844 issn: 2041-1723 databaseCode: PIMPY dateStart: 20100101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwEB7RFiQuvB-BsgoSN4ia2E5snxBFW4FEVxEq0sLFShwbKqFs2U0R_HtmnEe1PHrhkkPsRI5nxvPFnvkG4BlHlMxSVSVFbVUickpWtt4nokZvUmufCRsShd_JxUItl7ocNtw2Q1jluCaGhbpZWdojP2DoOBEq5IV-efYtoapRdLo6lNDYgT1iSWAhdK-c9liI_VwJMeTKpFwdbERYGeifKSPonrAtfxRo-_-GNf8Mmfzt3DS4o6Ob__sht-DGAETjV73m3IYrrr0D1_rSlD_vggy0VT-6mFIxKZwI0Xq88jHixXh-fBJ35OPGFQffs-5jbd09-HA0P3n9JhmKLCQWJ6BLLA6nttYXREXE8kprp7xHIESkwaljOa9Vw1mlLKu0SmubZw32l7lDMWrH-H3YbVetewgxV02DANBLpzPRNJSJWFiuRFVljXaNiCAbp9rYgYGcCmF8NeEknCvTi8egeEwQj2ERPJ-eOev5Ny7tfUgSnHoSd3a4sVp_NoMpGlTCwnoula6ckA1qK0-tLqytK4t4ykewPwrODAa9MRdSi-Dp1IymSOcrVetW532fAn8QizSCB726TCPhlJKcM5wDuaVIW0PdbmlPvwS6b0mkfHkRwYtR5S6G9e-peHT5VzyG64ysIIRu7sNutz53T-Cq_d6dbtYz2JFLGa5qBnuH80X5fhZ2K2bBwPBa5p-wpXx7XH78BbqyKgI
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwEB5VWxBceD8CBYIEJ4iaOE5iHxDi0aqr7q72sEjl5CaODZVQUnZToH-K38iM86iWR289cN040azzzSue-QbgWYxRMgtFHqSFFgFPqFlZWxvwAr1JIW3EtWsUnmSzmTg4kPMN-Nn3wlBZZW8TnaEua03fyLcZOk4MFZJUvj7-GtDUKDpd7UdotLDYN6ffMWVbvRq_x_f7nLHdncW7vaCbKhBoDNabQOODCq1tStw7LMmlNMJa9PzEkhsalsSFKGOWC81yKcJCJ1GJ67PEoNzSENEBmvxNTmAfweZ8PJ1_HL7qEN-64Lzrzgljsb3izhZRlhZRshCwNQ_oBgX8Lbr9s0jzt5Na5wB3r_9vW3cDrnWhtv-m1Y2bsGGqW3C5Hb55ehsyR8z1o_Gp2ZQKpjAf8WvrY0Ts70wXfkNevLep-JxlW01s7sCHC5H6LoyqujL3wY9FWWKIazMjI16W1GuZ6ljwPI9KaUruQdS_WqU7jnUa9fFFubP-WKgWDgrhoBwcFPPgxXDPccswcu7qt4SYYSWxg7sf6uUn1RkbhWqWahtnQuaGZyXqYxxqmWpd5BojRuvBVg8U1ZmslTpDiQdPh8tobOgEKa9MfdKuSTEFTkMP7rXwHCSJqek6YbgH2Rpw10Rdv1IdfXaE5hnRDiapBy97iJ-J9e-teHD-v3gCV_YW04majGf7D-EqIw10hapbMGqWJ-YRXNLfmqPV8nGnxD4cXjT4fwGENoFS
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9QwFH6qyiIu7EugQJDgBNEkjpPYB4SAdkTVMppDkSoubuIFKqGkzKRA_xq_jvecpRqW3nrgmjiR43xvs9_7HsDTFL1kFosyyistIp5RsbJ2LuIVWpNKuoRrXyi8W8xmYn9fztfg51ALQ2mVg070ito0mvbIJwwNJ7oKWS4nrk-LmG9OXx19jaiDFJ20Du00Oojs2JPvGL4tX25v4r9-xth0a-_tu6jvMBBpdNzbSONLK61dTjw8LCultMI59AKIMTe2LEsrYVJWCs1KKeJKZ4nB8UVm8RukJdIDVP8XCowxKZ1wnn0c93eIeV1w3tfpxKmYLLnXShSvJRQ2RGzFFvqWAX_zc_9M1_ztzNabwum1_3kRr8PV3gEPX3cScwPWbH0TLnUtOU9uQeHpun60IZWgUhoVRilh40L0k8Ot93thS7Z90LT4nkWXY2xvw4dzmfUdWK-b2t6DMBXGoOPrCisTbgxVYOY6FbwsEyOt4QEkw29WumdepwYgX5TPAEiF6qChEBrKQ0OxAJ6Pzxx1vCNnjn5D6BlHEme4v9AsPqleBSkUvly7tBCytLwwKKVprGWudVVq9CNdABsDaFSvyJbqFDEBPBlvowqic6Wyts1xNybHwDiPA7jbQXWcSUql2BnDNShWQLwy1dU79eFnT3NeEBlhlgfwYoD76bT-vRT3z_6Kx3AZEa92t2c7D-AKI2H02asbsN4uju1DuKi_tYfLxSMvzSEcnDfyfwEMEYi1
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Context+specificity+of+the+EMT+transcriptional+response&rft.jtitle=Nature+communications&rft.au=Cook%2C+David+P.&rft.au=Vanderhyden%2C+Barbara+C.&rft.date=2020-05-01&rft.issn=2041-1723&rft.eissn=2041-1723&rft.volume=11&rft.issue=1&rft_id=info:doi/10.1038%2Fs41467-020-16066-2&rft.externalDBID=n%2Fa&rft.externalDocID=10_1038_s41467_020_16066_2
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2041-1723&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2041-1723&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2041-1723&client=summon