CMOS-based bio-image sensor spatially resolves neural activity-dependent proton dynamics in the living brain

Recent studies have shown that protons can function as neurotransmitters in cultured neurons. To further investigate regional and neural activity-dependent proton dynamics in the brain, the development of a device with both wide-area detectability and high spatial-ltemporal resolution is necessary....

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Published in:Nature communications Vol. 11; no. 1; pp. 712 - 9
Main Authors: Horiuchi, Hiroshi, Agetsuma, Masakazu, Ishida, Junko, Nakamura, Yusuke, Lawrence Cheung, Dennis, Nanasaki, Shin, Kimura, Yasuyuki, Iwata, Tatsuya, Takahashi, Kazuhiro, Sawada, Kazuaki, Nabekura, Junichi
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 05.02.2020
Nature Publishing Group
Nature Portfolio
Subjects:
631/1647/1888
631/1647/245
9/10
Animal models
Animals
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Brain
Brain - diagnostic imaging
Brain - physiology
Brain Chemistry
Change detection
CMOS
Equipment Design
Humanities and Social Sciences
Hydrogen-Ion Concentration
Male
Measurement methods
Mice, Inbred C57BL
multidisciplinary
Neurotransmitters
pH effects
Photic Stimulation
Protons
Regional development
Science
Science (multidisciplinary)
Sensors
Spatio-Temporal Analysis
Stimulation
Temporal lobe
Temporal resolution
Visual cortex
Visual Cortex - diagnostic imaging
Visual Cortex - physiology
Visual stimuli
ISSN:2041-1723, 2041-1723
Online Access:Get full text
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Summary:Recent studies have shown that protons can function as neurotransmitters in cultured neurons. To further investigate regional and neural activity-dependent proton dynamics in the brain, the development of a device with both wide-area detectability and high spatial-ltemporal resolution is necessary. Therefore, we develop an image sensor with a high spatial-temporal resolution specifically designed for measuring protons in vivo. Here, we demonstrate that spatially deferent neural stimulation by visual stimulation induced distinct patterns of proton changes in the visual cortex. This result indicates that our biosensor can detect micrometer and millisecond scale changes of protons across a wide area. Our study demonstrates that a CMOS-based proton image sensor with high spatial and temporal precision can be used to detect pH changes associated with biological events. We believe that our sensor may have broad applicability in future biological studies. Protons have been discovered to play a role in neuronal signaling, but current methods to measure pH in the brain of animal models are limited. Here the authors develop a miniaturized proton image sensor that fits into a living mouse brain and can measure pH changes at micrometer and millisecond resolution scales.
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-020-14571-y