An evaluation of COVID-19 serological assays informs future diagnostics and exposure assessment

The world is entering a new era of the COVID-19 pandemic in which there is an increasing call for reliable antibody testing. To support decision making on the deployment of serology for either population screening or diagnostics, we present a detailed comparison of serological COVID-19 assays. We sh...

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Bibliographic Details
Published in:Nature communications Vol. 11; no. 1; pp. 3436 - 5
Main Authors: GeurtsvanKessel, Corine H., Okba, Nisreen M. A., Igloi, Zsofia, Bogers, Susanne, Embregts, Carmen W. E., Laksono, Brigitta M., Leijten, Lonneke, Rokx, Casper, Rijnders, Bart, Rahamat-Langendoen, Janette, van den Akker, Johannes P. C., van Kampen, Jeroen J. A., van der Eijk, Annemiek A., van Binnendijk, Rob S., Haagmans, Bart, Koopmans, Marion
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 06.07.2020
Nature Publishing Group
Nature Portfolio
Subjects:
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Antibodies
Antibodies, Neutralizing - blood
Antibodies, Viral - blood
Assaying
Betacoronavirus
Clinical Laboratory Techniques
Coronavirus Infections - diagnosis
Coronaviruses
COVID-19
COVID-19 Testing
Decision making
Enzyme-Linked Immunosorbent Assay
High-Throughput Screening Assays
Humanities and Social Sciences
Humans
Immunoglobulins
Luminescent Measurements
multidisciplinary
Neutralization
Neutralization Tests
Pandemics
Pneumonia, Viral - diagnosis
SARS-CoV-2
Science
Science (multidisciplinary)
Sensitivity and Specificity
Serologic Tests - standards
Serology
Severe acute respiratory syndrome coronavirus 2
Viral diseases
Viruses
ISSN:2041-1723, 2041-1723
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Summary:The world is entering a new era of the COVID-19 pandemic in which there is an increasing call for reliable antibody testing. To support decision making on the deployment of serology for either population screening or diagnostics, we present a detailed comparison of serological COVID-19 assays. We show that among the selected assays there is a wide diversity in assay performance in different scenarios and when correlated to virus neutralizing antibodies. The Wantai ELISA detecting total immunoglobulins against the receptor binding domain of SARS CoV-2, has the best overall characteristics to detect functional antibodies in different stages and severity of disease, including the potential to set a cut-off indicating the presence of protective antibodies. The large variety of available serological assays requires proper assay validation before deciding on deployment of assays for specific applications. SARS-CoV-2 is causing a global pandemic in which the implementation of serology can support decision making in different scenarios. Here, the authors compare the outcome of eight commercially available assays to virus neutralization and discuss their use in diagnostics and exposure assessment of SARS-CoV-2.
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-020-17317-y