Population-scale study of eRNA transcription reveals bipartite functional enhancer architecture

Enhancer RNAs (eRNA) are unstable non-coding RNAs, transcribed bidirectionally from active regulatory sequences, whose expression levels correlate with enhancer activity. We use capped-nascent-RNA sequencing to efficiently capture bidirectional transcription initiation across several human lymphobla...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Nature communications Ročník 11; číslo 1; s. 5963 - 12
Hlavní autoři: Kristjánsdóttir, Katla, Dziubek, Alexis, Kang, Hyun Min, Kwak, Hojoong
Médium: Journal Article
Jazyk:angličtina
Vydáno: London Nature Publishing Group UK 24.11.2020
Nature Publishing Group
Nature Portfolio
Témata:
38/39
38/43
45/91
631/208/200
631/208/726/2102
631/208/729/743
Bias
Binding
Binding sites
Cell Line
Enhancer Elements, Genetic - genetics
Enhancers
Gene expression
Gene Expression Regulation - genetics
Gene mapping
Gene sequencing
Humanities and Social Sciences
Humans
Lymphoblastoid cell lines
multidisciplinary
Non-coding RNA
Population
Population studies
Population-based studies
Quantitative Trait Loci
Regulatory sequences
Regulatory Sequences, Ribonucleic Acid
RNA polymerase
RNA, Messenger - metabolism
RNA, Untranslated - genetics
Science
Science (multidisciplinary)
Transcription factors
Transcription, Genetic
ISSN:2041-1723, 2041-1723
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Enhancer RNAs (eRNA) are unstable non-coding RNAs, transcribed bidirectionally from active regulatory sequences, whose expression levels correlate with enhancer activity. We use capped-nascent-RNA sequencing to efficiently capture bidirectional transcription initiation across several human lymphoblastoid cell lines (Yoruba population) and detect ~75,000 eRNA transcription sites with high sensitivity and specificity. The use of nascent-RNA sequencing sidesteps the confounding effect of eRNA instability. We identify quantitative trait loci (QTLs) associated with the level and directionality of eRNA expression. High-resolution analyses of these two types of QTLs reveal distinct positions of enrichment at the central transcription factor (TF) binding regions and at the flanking eRNA initiation regions, both of which are associated with mRNA expression QTLs. These two regions—the central TF-binding footprint and the eRNA initiation cores—define a bipartite architecture of enhancers, inform enhancer function, and can be used as an indicator of the significance of non-coding regulatory variants. Enhancer RNAs are transcribed bidirectionally from core transcription initiation regions. Here, by employing nascent RNA sequencing, the authors identify quantitative trait loci (QTLs) associated with enhancer RNA level and directionality, revealing the bipartite architecture of enhancers.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-020-19829-z