Dynamics of viral DNA shedding and culture viral DNA positivity in different clinical samples collected during the 2022 mpox outbreak in Lombardy, Italy

Mpox virus (MPXV) has recently spread outside of sub-Saharan Africa. This large multicentre study was conducted in Lombardy, the most densely populated Italian region accounting for more than 40% of Italian cases. The present study aims to: i) evaluate the presence and the shedding duration of MPXV...

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Vydáno v:	Travel medicine and infectious disease Ročník 59; s. 102698
Hlavní autoři:	Piralla, Antonio, Mileto, Davide, Rizzo, Alberto, Ferrari, Guglielmo, Giardina, Federica, Gaiarsa, Stefano, Petazzoni, Greta, Bianchi, Micol, Salari, Federica, Bracchitta, Fiorenza, Sammartino, Josè Camilla, Ferrari, Alessandro, Gagliardi, Gloria, Mancon, Alessandro, Fenizia, Claudio, Biasin, Mara, Rovida, Francesca, Paolucci, Stefania, Percivalle, Elena, Lombardi, Alessandra, Micheli, Valeria, Nozza, Silvia, Castagna, Antonella, Moschese, Davide, Antinori, Spinello, Gori, Andrea, Bonfanti, Paolo, Rossotti, Roberto, D'Arminio Monforte, Antonella, Attanasi, Federica, Tirani, Marcello, Cereda, Danilo, Baldanti, Fausto, Gismondo, Maria Rita, Cutrera, Miriam, Cuomo, Marianna, De Poli, Federica, Campanini, Giulia, Pitrolo, Antonino Maria Guglielmo, Iskandar, Elizabeth, Cassaniti, Irene, Bruno, Raffaele, Rizzardini, Giuliano, Puoti, Massimo, Castelli, Francesco, Corsico, Laura, Giacomelli, Andrea, Pozza, Giacomo, Casalini, Giacomo, Raccagni, Angelo, Trentacapilli, Bendetta, Bertoni, Costanza, Bruzzesi, Elena, Candela, Caterina, Tesoro, Daniele, Mule, Giovanni, Bandera, Alessandra, Bianca Mariani, Antonio Muscatello, Maffeo, Manuel, Vecchio, Riccardo, Piccinelli, Sara
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier Ltd 01.05.2024 Elsevier Limited Elsevier
Témata:	Adolescent Adult Aged Bibliometrics Cell culture Child Deoxyribonucleic acid Disease Outbreaks Disease transmission DNA DNA, Viral - genetics Epidemics Female Genomes Humans Infections Infectious Disease Italy - epidemiology Lesions Male Middle Aged Molecular epidemiology Mpox Mpox (monkeypox) Mpox virus Multiple samples Mutation Next generation sequencing Outbreaks Phylogenetics Phylogeny Picornaviridae Infections - epidemiology Picornaviridae Infections - virology Population density Re-Emerging virus Sequencing Sexually transmitted diseases Skin Software STD Survival analysis Virology Virus Shedding Viruses Whole Genome Sequencing Young Adult Italy Lombardy Italy Mpox virus Molecular epidemiology Multiple samples Re-Emerging virus Next generation sequencing
ISSN:	1477-8939, 1873-0442, 1873-0442
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Shrnutí:	Mpox virus (MPXV) has recently spread outside of sub-Saharan Africa. This large multicentre study was conducted in Lombardy, the most densely populated Italian region accounting for more than 40% of Italian cases. The present study aims to: i) evaluate the presence and the shedding duration of MPXV DNA in different body compartments correlating the MPXV viability with the time to onset of symptoms; ii) provide evidence of MPXV persistence in different body compartment as a source of infection and iii) characterize the MPXV evolution by whole genome sequencing (WGS) during the outbreak occurred in Italy. The study included 353 patients with a laboratory-confirmed diagnosis of MPXV infection screened in several clinical specimens in the period May 24th - September 1st, 2022. Viral isolation was attempted from different biological matrices and complete genome sequencing was performed for 61 MPXV strains. MPXV DNA detection was more frequent in the skin (94.4%) with the longest median time of viral clearance (16 days). The actively-replicating virus in cell culture was obtained for 123/377 (32.6%) samples with a significant higher viral quantity on isolation positive samples (20 vs 31, p < 0.001). The phylogenetic analysis highlighted the high genetic identity of the MPXV strains collected, both globally and within the Lombardy region. Skin lesion is gold standard material and the high viral load and the actively-replicating virus observed in genital sites confirms that sexual contact plays a key role in the viral transmission. •MPXV detection was more frequent from the skin lesions (289 of 306, 94·4%).•The median clearance time of MPXV DNA was 16 days in the skin, 14 days in the oropharynx.•Higher isolation rate was observed in semen, urine, anogenital, urethral and skin samples.•Higher isolation rate was observed in samples collected between 0 and 7 days (65/158, 41.1%) from the onset of symptoms.•Our 61 genomes are distributed in 37 local subclusters of the outbreak clade.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1477-8939 1873-0442 1873-0442
DOI:	10.1016/j.tmaid.2024.102698