Evolution of plant virus movement proteins from the 30K superfamily and of their homologs integrated in plant genomes

Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K su...

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Published in:Virology (New York, N.Y.) Vol. 476; pp. 304 - 315
Main Authors: Mushegian, Arcady R., Elena, Santiago F.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01.02.2015
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ISSN:0042-6822, 1096-0341, 1096-0341
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Abstract Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K superfamily with the broadest phylogenetic coverage thus far and illuminating structural features of the core all-beta fold of these proteins. Integrated copies of pararetrovirus 30K movement genes are prevalent in euphyllophytes, with at least one copy intact in nearly every examined species, and mRNAs detected for most of them. Sequence analysis suggests repeated integrations, pseudogenizations, and positive selection in those provirus genes. An unannotated 30K-superfamily gene in Arabidopsis thaliana genome is likely expressed as a fusion with the At1g37113 transcript. This molecular background of endopararetrovirus gene products in plants may change our view of virus infection and pathogenesis, and perhaps of cellular homeostasis in the hosts. •Sequence region shared by plant virus “30K” movement proteins has an all-beta fold.•Most euphyllophyte genomes contain integrated copies of pararetroviruses.•These integrated virus genomes often include intact movement protein genes.•Molecular evidence suggests that these “30K” genes may be selected for function.
AbstractList Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K superfamily with the broadest phylogenetic coverage thus far and illuminating structural features of the core all-beta fold of these proteins. Integrated copies of pararetrovirus 30K movement genes are prevalent in euphyllophytes, with at least one copy intact in nearly every examined species, and mRNAs detected for most of them. Sequence analysis suggests repeated integrations, pseudogenizations, and positive selection in those provirus genes. An unannotated 30K-superfamily gene in Arabidopsis thaliana genome is likely expressed as a fusion with the At1g37113 transcript. This molecular background of endopararetrovirus gene products in plants may change our view of virus infection and pathogenesis, and perhaps of cellular homeostasis in the hosts.Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K superfamily with the broadest phylogenetic coverage thus far and illuminating structural features of the core all-beta fold of these proteins. Integrated copies of pararetrovirus 30K movement genes are prevalent in euphyllophytes, with at least one copy intact in nearly every examined species, and mRNAs detected for most of them. Sequence analysis suggests repeated integrations, pseudogenizations, and positive selection in those provirus genes. An unannotated 30K-superfamily gene in Arabidopsis thaliana genome is likely expressed as a fusion with the At1g37113 transcript. This molecular background of endopararetrovirus gene products in plants may change our view of virus infection and pathogenesis, and perhaps of cellular homeostasis in the hosts.
Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K superfamily with the broadest phylogenetic coverage thus far and illuminating structural features of the core all-beta fold of these proteins. Integrated copies of pararetrovirus 30K movement genes are prevalent in euphyllophytes, with at least one copy intact in nearly every examined species, and mRNAs detected for most of them. Sequence analysis suggests repeated integrations, pseudogenizations, and positive selection in those provirus genes. An unannotated 30K-superfamily gene in Arabidopsis thaliana genome is likely expressed as a fusion with the At1g37113 transcript. This molecular background of endopararetrovirus gene products in plants may change our view of virus infection and pathogenesis, and perhaps of cellular homeostasis in the hosts.
Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K superfamily with the broadest phylogenetic coverage thus far and illuminating structural features of the core all-beta fold of these proteins. Integrated copies of pararetrovirus 30K movement genes are prevalent in euphyllophytes, with at least one copy intact in nearly every examined species, and mRNAs detected for most of them. Sequence analysis suggests repeated integrations, pseudogenizations, and positive selection in those provirus genes. An unannotated 30K-superfamily gene in Arabidopsis thaliana genome is likely expressed as a fusion with the At1g37113 transcript. This molecular background of endopararetrovirus gene products in plants may change our view of virus infection and pathogenesis, and perhaps of cellular homeostasis in the hosts. - Highlights: • Sequence region shared by plant virus “30K” movement proteins has an all-beta fold. • Most euphyllophyte genomes contain integrated copies of pararetroviruses. • These integrated virus genomes often include intact movement protein genes. • Molecular evidence suggests that these “30K” genes may be selected for function.
Abstract Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K superfamily with the broadest phylogenetic coverage thus far and illuminating structural features of the core all-beta fold of these proteins. Integrated copies of pararetrovirus 30K movement genes are prevalent in euphyllophytes, with at least one copy intact in nearly every examined species, and mRNAs detected for most of them. Sequence analysis suggests repeated integrations, pseudogenizations, and positive selection in those provirus genes. An unannotated 30K-superfamily gene in Arabidopsis thaliana genome is likely expressed as a fusion with the At1g37113 transcript. This molecular background of endopararetrovirus gene products in plants may change our view of virus infection and pathogenesis, and perhaps of cellular homeostasis in the hosts.
Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these proteins remain obscure. We expand the picture of conservation and evolution of the 30K proteins, producing sequence alignment of the 30K superfamily with the broadest phylogenetic coverage thus far and illuminating structural features of the core all-beta fold of these proteins. Integrated copies of pararetrovirus 30K movement genes are prevalent in euphyllophytes, with at least one copy intact in nearly every examined species, and mRNAs detected for most of them. Sequence analysis suggests repeated integrations, pseudogenizations, and positive selection in those provirus genes. An unannotated 30K-superfamily gene in Arabidopsis thaliana genome is likely expressed as a fusion with the At1g37113 transcript. This molecular background of endopararetrovirus gene products in plants may change our view of virus infection and pathogenesis, and perhaps of cellular homeostasis in the hosts. •Sequence region shared by plant virus “30K” movement proteins has an all-beta fold.•Most euphyllophyte genomes contain integrated copies of pararetroviruses.•These integrated virus genomes often include intact movement protein genes.•Molecular evidence suggests that these “30K” genes may be selected for function.
Author Elena, Santiago F.
Mushegian, Arcady R.
Author_xml – sequence: 1
  givenname: Arcady R.
  surname: Mushegian
  fullname: Mushegian, Arcady R.
  email: mushegian2@gmail.com
  organization: Division of Molecular and Cellular Biosciences, National Science Foundation, 4201 Wilson Boulevard, Arlington, VA 22230, USA
– sequence: 2
  givenname: Santiago F.
  surname: Elena
  fullname: Elena, Santiago F.
  email: sfelena@ibmcp.upv.es
  organization: Instituto de Biología Molecular y Celular de Plantas, CSIC-UPV, 46022 València, Spain
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Keywords Endopararetroviruses
Virus evolution
Virus cell-to-cell movement
Plasmodesmata
Virus to host gene transfer
Language English
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  publication-title: PLoS Genet.
  doi: 10.1371/journal.pgen.1002764
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Snippet Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins of these...
Abstract Homologs of Tobacco mosaic virus 30K cell-to-cell movement protein are encoded by diverse plant viruses. Mechanisms of action and evolutionary origins...
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SubjectTerms 60 APPLIED LIFE SCIENCES
ALIGNMENT
Amino Acid Sequence
ARABIDOPSIS
Arabidopsis thaliana
Endopararetroviruses
EVOLUTION
Evolution, Molecular
GENES
Genome, Plant
HOMEOSTASIS
Host Specificity
hosts
Infectious Disease
mechanism of action
MESSENGER-RNA
Molecular Sequence Data
Multigene Family
PATHOGENESIS
Phylogeny
Plant Viral Movement Proteins - chemistry
Plant Viral Movement Proteins - genetics
Plant Viral Movement Proteins - metabolism
plant viruses
Plant Viruses - chemistry
Plant Viruses - classification
Plant Viruses - genetics
Plant Viruses - physiology
Plants - classification
Plants - genetics
Plants - virology
Plasmodesmata
PROTEINS
proviruses
Sequence Alignment
STRUCTURAL CHEMICAL ANALYSIS
TOBACCO MOSAIC VIRUS
Virus cell-to-cell movement
Virus evolution
Virus Integration
Virus to host gene transfer
Title Evolution of plant virus movement proteins from the 30K superfamily and of their homologs integrated in plant genomes
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https://dx.doi.org/10.1016/j.virol.2014.12.012
https://www.ncbi.nlm.nih.gov/pubmed/25576984
https://www.proquest.com/docview/1653128394
https://www.proquest.com/docview/1660417120
https://www.proquest.com/docview/2985762235
https://www.osti.gov/biblio/22470155
Volume 476
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