A decade of metaproteomics: Where we stand and what the future holds

We are living through exciting times during which we are able to unravel the “microbial dark matter” in and around us through the application of high‐resolution “meta‐omics”. Metaproteomics offers the ability to resolve the major catalytic units of microbial populations and thereby allows the establ...

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Vydané v:	Proteomics (Weinheim) Ročník 15; číslo 20; s. 3409 - 3417
Hlavní autori:	Wilmes, Paul, Heintz-Buschart, Anna, Bond, Philip L.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Germany Blackwell Publishing Ltd 01.10.2015 Wiley Subscription Services, Inc John Wiley and Sons Inc
Predmet:	Acid mine drainage Activated sludge Betaproteobacteria - genetics Biofilms Catalysis Chemical analysis Communities Community composition Cyanobacteria - genetics Dark matter Ecological function Ecosystem Fresh Water Genotypes Humans Integrated omics Intestinal microflora Leptospiraceae - genetics Metagenomics Metaproteomics Microbial activity Microbial community Microbial systems ecology Microbiology Microbiota Microorganisms Phenotypes Proteins Proteome - genetics Proteomics Seawater Soil Microbiology Viewpoint Water analysis Metagenomics Microbial systems ecology Metaproteomics Microbiology Integrated omics Microbial community
ISSN:	1615-9853, 1615-9861, 1615-9861
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Shrnutí:	We are living through exciting times during which we are able to unravel the “microbial dark matter” in and around us through the application of high‐resolution “meta‐omics”. Metaproteomics offers the ability to resolve the major catalytic units of microbial populations and thereby allows the establishment of genotype‐phenotype linkages from in situ samples. A decade has passed since the term “metaproteomics” was first coined and corresponding analyses were carried out on mixed microbial communities. Since then metaproteomics has yielded many important insights into microbial ecosystem function in the various environmental settings where it has been applied. Although initial progress in analytical capacities and resulting numbers of proteins identified was extremely fast, this trend slowed rapidly. Here, we discuss several representative metaproteomic investigations of activated sludge, acid mine drainage biofilms, freshwater and seawater microbial communities, soil, and human gut microbiota. By using these case studies, we highlight current challenges and possible solutions for metaproteomics to realize its full potential, i.e. to enable conclusive links between microbial community composition, physiology, function, interactions, ecology, and evolution in situ.
Bibliografia:	ark:/67375/WNG-13J8VB60-T ArticleID:PMIC12114 istex:E6122B6B398ECB6D70D874FEB6E19F7B48A2AB66 phil.bond@awmc.uq.edu.au E‐mail Additional corresponding author: Dr. Philip L. Bond ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 E‐mail: phil.bond@awmc.uq.edu.au
ISSN:	1615-9853 1615-9861 1615-9861
DOI:	10.1002/pmic.201500183