Does soaking temperature during controlled slow freezing of pre-pubertal mouse testes influence course of in vitro spermatogenesis?

The banking of testicular tissue before highly gonadotoxic treatment is a prerequisite for the preservation of fertility in pre-pubertal boys not yet producing sperm. The aim of the current study is to evaluate the impact of a soaking temperature performed at −7 °C, −8 °C or −9 °C on the ability of...

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Bibliographic Details
Published in:	Cell and tissue research Vol. 364; no. 3; pp. 661 - 674
Main Authors:	Arkoun, Brahim, Dumont, Ludovic, Milazzo, Jean-Pierre, Rondanino, Christine, Bironneau, Amandine, Wils, Julien, Rives, Nathalie
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2016 Springer Springer Nature B.V Springer Verlag
Subjects:	agarose Animals banking Banks (Finance) Biomedical and Life Sciences Biomedicine Boys Cancer Cell Count Cell Differentiation Cell Proliferation Cryopreservation Epithelium - anatomy & histology Freezing gels haploidy Human Genetics Humans in vitro culture Leydig Cells - cytology Life Sciences Male Medical research Mice Molecular Medicine Pediatrics Proteomics Regular Article Reproductive Biology Seminiferous Tubules - anatomy & histology Sertoli Cells - cytology Sexual Maturation soaking spermatids Spermatids - cytology Spermatogenesis spermatozoa staining Stem cells Stem Cells - cytology temperature testes Testis - physiology Testosterone - metabolism Tissue Culture Techniques tissues In vitro spermatogenesis Controlled slow freezing Spermatogonial stem cells Soaking temperature Testicular tissue
ISSN:	0302-766X, 1432-0878, 1432-0878
Online Access:	Get full text
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