Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium

Extracellular RNAs (exRNAs) have been identified in all tested biofluids and have been associated with a variety of extracellular vesicles, ribonucleoprotein complexes and lipoprotein complexes. Much of the interest in exRNAs lies in the fact that they may serve as signalling molecules between cells...

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Published in:Journal of extracellular vesicles Vol. 4; no. 1; pp. 26533 - n/a
Main Authors: Laurent, Louise C., Abdel-Mageed, Asim B., Adelson, P. David, Arango, Jorge, Balaj, Leonora, Breakefield, Xandra, Carlson, Elizabeth, Carter, Bob S., Majem, Blanca, Chen, Clark C., Cocucci, Emanuele, Danielson, Kirsty, Courtright, Amanda, Das, Saumya, Elmageed, Zakaria Y. Abd, Enderle, Daniel, Ezrin, Alan, Ferrer, Marc, Freedman, Jane, Galas, David, Gandhi, Roopali, Huentelman, Matthew J., Van Keuren-Jensen, Kendall, Kalani, Yashar, Kim, Yong, Krichevsky, Anna M., Lai, Charles, Lal-Nag, Madhu, Laurent, Clara D., Leonardo, Trevor, Li, Feng, Malenica, Ivana, Mondal, Debasis, Nejad, Parham, Patel, Tushar, Raffai, Robert L., Rubio, Renee, Skog, Johan, Spetzler, Robert, Sun, Jie, Tanriverdi, Kahraman, Vickers, Kasey, Wang, Liang, Wang, Yaoyu, Wei, Zhiyun, Weiner, Howard L., Wong, David, Yan, Irene K., Yeri, Ashish, Gould, Stephen
Format: Journal Article
Language:English
Published: Sweden Taylor & Francis 01.01.2015
John Wiley & Sons, Inc
Co-Action Publishing
Wiley
Subjects:
Anticoagulants
Atoms & subatomic particles
Bias
Biological activity
Biomarkers
Biosynthesis
Cancer
Cell interactions
Communication
Consortia
Disease
exosomes
extracellular RNA
extracellular vesicles
Flow cytometry
Funding
Gene expression
Genomics
Laboratories
Libraries
Library collections
Medicine
MicroRNAs
microvesicles
Plasma
Reproductive health
Ribonucleic acid
RNA
RNA processing
RNA sequencing
Signal processing
Special Issue: Extracellular RNA Communication Consortium
Studies
Therapeutic applications
Thrombosis
Working groups
United States > US
RNA sequencing
extracellular vesicles
microvesicles
exosomes
extracellular RNA
ISSN:2001-3078, 2001-3078
Online Access:Get full text
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Summary:Extracellular RNAs (exRNAs) have been identified in all tested biofluids and have been associated with a variety of extracellular vesicles, ribonucleoprotein complexes and lipoprotein complexes. Much of the interest in exRNAs lies in the fact that they may serve as signalling molecules between cells, their potential to serve as biomarkers for prediction and diagnosis of disease and the possibility that exRNAs or the extracellular particles that carry them might be used for therapeutic purposes. Among the most significant bottlenecks to progress in this field is the lack of robust and standardized methods for collection and processing of biofluids, separation of different types of exRNA-containing particles and isolation and analysis of exRNAs. The Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium is a group of laboratories funded by the U.S. National Institutes of Health to develop such methods. In our first joint endeavour, we held a series of conference calls and in-person meetings to survey the methods used among our members, placed them in the context of the current literature and used our findings to identify areas in which the identification of robust methodologies would promote rapid advancements in the exRNA field.
Bibliography:More papers from this issue can be found at
http://www.journalofextracellularvesicles.net
This paper is part of the Special Issue
Extracellular RNA Communication Consortium
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This paper is part of the Special Issue: Extracellular RNA Communication Consortium. More papers from this issue can be found at http://www.journalofextracellularvesicles.net
ISSN:2001-3078
2001-3078
DOI:10.3402/jev.v4.26533