Characterization of Zika virus binding and enhancement potential of a large panel of flavivirus murine monoclonal antibodies

Zika viruses (ZIKVs) are circulating in parts of the world endemic for other flavivirus infections. Some cross-reactive antibodies (Abs) elicited by prior flavivirus exposures can bind to ZIKV and enhance infection of Fc receptor-bearing cells. Here, we measured ZIKV binding of 54 murine monoclonal...

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Bibliographic Details
Published in:Virology (New York, N.Y.) Vol. 508; pp. 1 - 6
Main Authors: Willis, Elinor, Hensley, Scott E.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01.08.2017
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antibody
Antibody mediated enhancement
Cross Reactions
Dengue virus
Flavivirus
Flavivirus - immunology
Flavivirus Infections - immunology
Flavivirus Infections - virology
Humans
Infectious Disease
Mice
Viral Envelope Proteins - immunology
virology
West Nile virus
Zika virus
Zika Virus - immunology
Zika Virus Infection - immunology
Zika Virus Infection - virology
Zika virus
Antibody mediated enhancement
Antibody
Flavivirus
ISSN:0042-6822, 1096-0341, 1096-0341
Online Access:Get full text
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Summary:Zika viruses (ZIKVs) are circulating in parts of the world endemic for other flavivirus infections. Some cross-reactive antibodies (Abs) elicited by prior flavivirus exposures can bind to ZIKV and enhance infection of Fc receptor-bearing cells. Here, we measured ZIKV binding of 54 murine monoclonal Abs (mAbs) elicited by exposure with Dengue virus and West Nile virus antigens. We found that 8 of 54 mAbs recognized the envelope protein of ZIKV in conventional binding assays. These 8 cross-reactive mAbs have different specificities; most recognize the DI/II region of the envelope protein but one mAb recognized the DIII lateral ridge of the envelope protein. Interestingly, only 3 of these cross-reactive mAbs were able to enhance ZIKV infection in vitro, and enhancing potential was not strictly correlated with relative binding ability. These data suggest that the ability of flavivirus Abs to enhance ZIKV is dependent on multiple factors.
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ISSN:0042-6822
1096-0341
1096-0341
DOI:10.1016/j.virol.2017.04.031