Recent advances and challenges in the recovery and purification of cellular exosomes

Exosomes are nanovesicles secreted by most cellular types that carry important biochemical compounds throughout the body with different purposes, playing a preponderant role in cellular communication. Because of their structure, physicochemical properties and stability, recent studies are focusing i...

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Published in:Electrophoresis Vol. 40; no. 23-24; pp. 3036 - 3049
Main Authors: Ayala‐Mar, Sergio, Donoso‐Quezada, Javier, Gallo‐Villanueva, Roberto C., Perez‐Gonzalez, Victor H., González‐Valdez, José
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01.12.2019
John Wiley and Sons Inc
Subjects:
biochemical compounds
Bioseparations
Bioseparations 2019
Biotechnology - methods
Cells, Cultured
Cellular communication
Cellular structure
Cellular vesicles
Chemical compounds
Chemistry Techniques, Analytical - methods
Downstream processing
electrophoresis
Exosome purification
Exosomes
Humans
Microfluidic Analytical Techniques - methods
nanocarriers
neoplasms
Parkinson disease
Parkinson's disease
Pharmacology
physicochemical properties
Purification
Review
Exosome purification
Exosomes
Cellular vesicles
Bioseparations
Downstream processing
ISSN:0173-0835, 1522-2683, 1522-2683
Online Access:Get full text
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Summary:Exosomes are nanovesicles secreted by most cellular types that carry important biochemical compounds throughout the body with different purposes, playing a preponderant role in cellular communication. Because of their structure, physicochemical properties and stability, recent studies are focusing in their use as nanocarriers for different therapeutic compounds for the treatment of different diseases ranging from cancer to Parkinson's disease. However, current bioseparation protocols and methodologies are selected based on the final exosome application or intended use and present both advantages and disadvantages when compared among them. In this context, this review aims to present the most important technologies available for exosome isolation while discussing their advantages and disadvantages and the possibilities of being combined with other strategies. This is critical since the development of novel exosome‐based therapeutic strategies will be constrained to the effectiveness and yield of the selected downstream purification methodologies for which a thorough understanding of the available technological resources is needed.
Bibliography:These authors contributed equally to this work.
The copyright line for this article was changed on August 30, 2019 after original online publication.
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ISSN:0173-0835
1522-2683
1522-2683
DOI:10.1002/elps.201800526